Kinetic characterization of Channa striatus muscle sarcoplasmic and myofibrillar protein hydrolysates

Masomeh Ghassem, See Siau Fern, Mamot Said, Zainon Mohd Ali, Saadiah Ibrahim, Abd. Salam Babji

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

This study was conducted to evaluate the kinetic characteristics of proteolytic activity of proteases on Channa striatus protein fractions. Degree of hydrolysis (DH), amino acid composition and kinetic parameters of sarcoplasmic and myofibrillar proteins were investigated when incubated with proteinase K and thermolysin, separately. After 30 min incubation with proteases, a decrease in DH of sarcoplasmic protein was observed whereas, hydrolysis of myofibrillar protein with proteases took 2 h with an increase in DH. The major amino acids were glutamic acid (16.6%) in thermolysin- myofibrillar hydrolysate followed by aspartic acid (11.1%) in sarcoplasmic protein fraction with no enzyme treatment and lysine (10%) in thermolysin-myofibrillar hydrolysate. The apparent Michaelis constant of proteinase K was lower than thermolysin for both sarcoplasmic and myofibrillar proteins. However, rate of turnover and enzyme efficiency suggested that sarcoplasmic and myofibrillar proteins are suitable substrates for proteinase K and thermolysin hydrolytic reaction, respectively.

Original languageEnglish
Pages (from-to)467-475
Number of pages9
JournalJournal of Food Science and Technology
Volume51
Issue number3
DOIs
Publication statusPublished - Mar 2014

Fingerprint

Channa
Protein Hydrolysates
myofibrillar proteins
protein hydrolysates
Thermolysin
hydrolysis
kinetics
Muscles
muscles
proteinases
Endopeptidase K
hydrolysates
Hydrolysis
Proteins
Peptide Hydrolases
proteins
enzymatic treatment
aspartic acid
glutamic acid
amino acid composition

Keywords

  • Channa striatus
  • Degree of hydrolysis
  • Kinetic parameters
  • Michaelis constant
  • Proteolytic activity

ASJC Scopus subject areas

  • Food Science

Cite this

Kinetic characterization of Channa striatus muscle sarcoplasmic and myofibrillar protein hydrolysates. / Ghassem, Masomeh; Fern, See Siau; Said, Mamot; Ali, Zainon Mohd; Ibrahim, Saadiah; Babji, Abd. Salam.

In: Journal of Food Science and Technology, Vol. 51, No. 3, 03.2014, p. 467-475.

Research output: Contribution to journalArticle

Ghassem, Masomeh ; Fern, See Siau ; Said, Mamot ; Ali, Zainon Mohd ; Ibrahim, Saadiah ; Babji, Abd. Salam. / Kinetic characterization of Channa striatus muscle sarcoplasmic and myofibrillar protein hydrolysates. In: Journal of Food Science and Technology. 2014 ; Vol. 51, No. 3. pp. 467-475.
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