Jumonji domain containing protein 6 (Jmjd6) modulates splicing and specifically interacts with arginine-serine-rich (RS) domains of SR- and SR-like proteins

Astrid Heim, Christina Grimm, Udo Müller, Simon Häuler, Muhammad Mukram Mohamed Mackeen, Juliane Merl, Stefanie M. Hauck, Benedikt M. Kessler, Christopher J. Schofield, Alexander Wolf, Angelika Böttger

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

The Fe(II) and 2-oxoglutarate dependent oxygenase Jmjd6 has been shown to hydroxylate lysine residues in the essential splice factor U2 auxiliary factor 65 kDa subunit (U2AF65) and to act as a modulator of alternative splicing. We describe further evidence for the role of Jmjd6 in the regulation of pre-mRNA processing including interactions of Jmjd6 with multiple arginine-serine-rich (RS)-domains of SR- and SR-related proteins including U2AF65, Luc7-like protein 3 (Luc7L3), SRSF11 and Acinus S', but not with the bona fide RS-domain of SRSF1. The identified Jmjd6 target proteins are involved in different mRNA processing steps and play roles in exon dependent alternative splicing and exon definition. Moreover, we show that Jmjd6 modifies splicing of a constitutive splice reporter, binds RNA derived from the reporter plasmid and punctually co-localises with nascent RNA. We propose that Jmjd6 exerts its splice modulatory function by interacting with specific SR-related proteins during splicing in a RNA dependent manner.

Original languageEnglish
Pages (from-to)7833-7850
Number of pages18
JournalNucleic Acids Research
Volume42
Issue number12
DOIs
Publication statusPublished - 2014
Externally publishedYes

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Serine
Arginine
Proteins
Alternative Splicing
RNA
Exons
Protein Splicing
Oxygenases
RNA Precursors
Lysine
Protein Domains
Plasmids
Messenger RNA

ASJC Scopus subject areas

  • Genetics

Cite this

Jumonji domain containing protein 6 (Jmjd6) modulates splicing and specifically interacts with arginine-serine-rich (RS) domains of SR- and SR-like proteins. / Heim, Astrid; Grimm, Christina; Müller, Udo; Häuler, Simon; Mohamed Mackeen, Muhammad Mukram; Merl, Juliane; Hauck, Stefanie M.; Kessler, Benedikt M.; Schofield, Christopher J.; Wolf, Alexander; Böttger, Angelika.

In: Nucleic Acids Research, Vol. 42, No. 12, 2014, p. 7833-7850.

Research output: Contribution to journalArticle

Heim, A, Grimm, C, Müller, U, Häuler, S, Mohamed Mackeen, MM, Merl, J, Hauck, SM, Kessler, BM, Schofield, CJ, Wolf, A & Böttger, A 2014, 'Jumonji domain containing protein 6 (Jmjd6) modulates splicing and specifically interacts with arginine-serine-rich (RS) domains of SR- and SR-like proteins', Nucleic Acids Research, vol. 42, no. 12, pp. 7833-7850. https://doi.org/10.1093/nar/gku488
Heim, Astrid ; Grimm, Christina ; Müller, Udo ; Häuler, Simon ; Mohamed Mackeen, Muhammad Mukram ; Merl, Juliane ; Hauck, Stefanie M. ; Kessler, Benedikt M. ; Schofield, Christopher J. ; Wolf, Alexander ; Böttger, Angelika. / Jumonji domain containing protein 6 (Jmjd6) modulates splicing and specifically interacts with arginine-serine-rich (RS) domains of SR- and SR-like proteins. In: Nucleic Acids Research. 2014 ; Vol. 42, No. 12. pp. 7833-7850.
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