Javanese Medaka (Oryzias javanicus Bleeker, 1854) as Potential Model Organism for Aeromoniasis and Vibriosis Study in Fish

Mohammad Noor Azmai Amal, Mohd Fauzul Aidil Mohd Roseli, Jumria Sutra, Syuhada Roslan, Siti Nur Khamisah Mazlan, Lukman Basri, Lee Jing Yie, Nurliyana Mohamad, Ahmad Ismail, Mohd Zamri Saad, Ina Salwany Md Yasin, Nasruddin Nurrul Shaqinah

Research output: Contribution to journalArticle

Abstract

Javanese medaka is a small tropical fish that has been widely used as a test organism in ecology, toxicology and ecotoxicology, but less in fish-pathogen related study. This study evaluates the capability of Javanese medaka as an alternative model organism for aeromoniasis and vibriosis study in fish. Javanese medakas were infected with different concentrations of Aeromonas hydrophila, Vibrio alginolyticus and V. harveyi via intraperitoneal (IP) and immersion (IMM) routes. Following the infections, the LD50- 240h of all bacteria to Javanese medaka were observed lower in IP, compared with IMM route. In IP route, the LD50-240h ranged from 2.1 × 104 CFU/mL for V. harveyi to 2.5 × 107 CFU/mL for A. hydrophila. However, for IMM route, the range was from 6.6 × 107 CFU/mL for V. harveyi to 1.1 × 109 CFU/ mL for V. alginolyticus. The clinical signs, gross lesions and histopathological changes of Javanese medakas infected by either IP or IMM routes of all the pathogens were similarly observed in the real host of the pathogen. Moreover, in IP route, most of the fish mortalities (88.9%) were observed within ≤ 120 hours post infection (hpi), while for IMM, most of the mortalities (50.6%) were witnessed beyond 120 hpi, indicating the acute infection for IP compared to IMM route. This study highlights the potential utilization of Javanese medaka as another valuable in vivo model organism for bacterial diseases study in fish.

Original languageEnglish
Pages (from-to)1049-1065
Number of pages17
JournalPertanika Journal of Tropical Agricultural Science
Volume42
Issue number3
Publication statusPublished - 1 Jan 2019

Fingerprint

vibriosis
Vibrio alginolyticus
Aeromonas hydrophila
organisms
lethal dose 50
fish
infection
pathogens
Vibrio harveyi
tropical fish
ecotoxicology
toxicology
lesions (animal)
ecology
Oryzias javanicus
bacteria
testing

Keywords

  • Aquaculture
  • Fish diseases
  • Javanese medaka
  • Test organism

ASJC Scopus subject areas

  • Agronomy and Crop Science

Cite this

Javanese Medaka (Oryzias javanicus Bleeker, 1854) as Potential Model Organism for Aeromoniasis and Vibriosis Study in Fish. / Amal, Mohammad Noor Azmai; Roseli, Mohd Fauzul Aidil Mohd; Sutra, Jumria; Roslan, Syuhada; Mazlan, Siti Nur Khamisah; Basri, Lukman; Yie, Lee Jing; Mohamad, Nurliyana; Ismail, Ahmad; Saad, Mohd Zamri; Yasin, Ina Salwany Md; Nurrul Shaqinah, Nasruddin.

In: Pertanika Journal of Tropical Agricultural Science, Vol. 42, No. 3, 01.01.2019, p. 1049-1065.

Research output: Contribution to journalArticle

Amal, MNA, Roseli, MFAM, Sutra, J, Roslan, S, Mazlan, SNK, Basri, L, Yie, LJ, Mohamad, N, Ismail, A, Saad, MZ, Yasin, ISM & Nurrul Shaqinah, N 2019, 'Javanese Medaka (Oryzias javanicus Bleeker, 1854) as Potential Model Organism for Aeromoniasis and Vibriosis Study in Fish', Pertanika Journal of Tropical Agricultural Science, vol. 42, no. 3, pp. 1049-1065.
Amal, Mohammad Noor Azmai ; Roseli, Mohd Fauzul Aidil Mohd ; Sutra, Jumria ; Roslan, Syuhada ; Mazlan, Siti Nur Khamisah ; Basri, Lukman ; Yie, Lee Jing ; Mohamad, Nurliyana ; Ismail, Ahmad ; Saad, Mohd Zamri ; Yasin, Ina Salwany Md ; Nurrul Shaqinah, Nasruddin. / Javanese Medaka (Oryzias javanicus Bleeker, 1854) as Potential Model Organism for Aeromoniasis and Vibriosis Study in Fish. In: Pertanika Journal of Tropical Agricultural Science. 2019 ; Vol. 42, No. 3. pp. 1049-1065.
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AU - Roslan, Syuhada

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AU - Yie, Lee Jing

AU - Mohamad, Nurliyana

AU - Ismail, Ahmad

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AU - Yasin, Ina Salwany Md

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