Issues in identifying germ tube positive yeasts by conventional methods

Atta Yazdanpanah, Tzar Mohd Nizam Khaithir

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Candida speciation is vital for epidemiology and management of candidiasis. Nonmolecular conventional methods often fail to identify closely related germ tube positive yeasts from clinical specimens. The present study was conducted to identify these yeasts and to highlight issues in conventional versus molecular methods of identification. A total of 98 germ tube positive yeasts from high vaginal swabs were studied over a 12-month period. Isolates were examined with various methods including growth at 42°C and 45°C on Sabouraud dextrose agar (SDA), color development on CHROMagar Candida medium, chlamydospore production on corn meal agar at 25°C, carbohydrate assimilation using ID 32C system, and polymerase chain reaction using a single pair of primers targeting the hyphal wall protein 1 (Hwp1) gene. Of all the isolates studied, 97 were molecularly confirmed as C. albicans and one isolate was identified as C. dubliniensis. No C. africana was detected in this study. The molecular method used in our study was an accurate and useful tool for discriminating C. albicans, C. dubliniensis, and C. africana. The conventional methods, however, were less accurate and riddled with many issues that will be discussed in further details.

Original languageEnglish
Pages (from-to)1-9
Number of pages9
JournalJournal of Clinical Laboratory Analysis
Volume28
Issue number1
DOIs
Publication statusPublished - Jan 2014

Fingerprint

Yeast
Candida
Yeasts
Agar
Epidemiology
Polymerase chain reaction
Genes
Carbohydrates
Candidiasis
Color
Glucose
Zea mays
Meals
Proteins
Polymerase Chain Reaction
Growth

Keywords

  • C. africana
  • C. albicans
  • C. dubliniensis
  • Hwp1
  • Malaysia

ASJC Scopus subject areas

  • Medical Laboratory Technology
  • Microbiology (medical)
  • Immunology and Allergy
  • Hematology
  • Public Health, Environmental and Occupational Health
  • Biochemistry, medical
  • Clinical Biochemistry

Cite this

Issues in identifying germ tube positive yeasts by conventional methods. / Yazdanpanah, Atta; Khaithir, Tzar Mohd Nizam.

In: Journal of Clinical Laboratory Analysis, Vol. 28, No. 1, 01.2014, p. 1-9.

Research output: Contribution to journalArticle

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