Isoprenaline reverses glycyrrhizic acid - Induced inhibition of 11-hydroxysteroid dehydrogenase bioactivity

Farihah Suhaimi, Nabishah Mohamad, Khalid Bin Abdul Kadir

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Glycyrrhizic acid (GCA) exerts its effect by inhibiting 11beta-hydroxysteroid dehydrogenase (11 -HSD)which catalyses the oxidation of cortisol to cortisone in man and corticosterone (B) to 11-dehydrocorticosterone (A) in rats. This GCA induced inhibition of 11 -HSD activity can be overcome by repetitive stress. Since catecholamines are among the mediators of stress, this study was carried out to determine the effect of isoprenaline on GCA-induced inhibition 11 -HSD bioactivity. Intact male Sprague Dawley rats received either drinking solution containing 1 mg/ml GCA or tap water for 10 days. On the day of killing, the rats received an intraperitoneal injection of vehicle, isoprenaline or propranolol. Hypothalamus, liver and kidney homogenates were assayed for 11 -HSD1 and 11 -HSD2 bioactivity by determining the percentage conversion of B to A in the presence of NADP and NAD respectively. Isoprenaline or propranolol had no effect on basal activity of 11 -HSD in all tissues. Isoprenaline reversed the GCA induced inhibition of 11 -HSD1 activity in the hypothalamus and kidney, whereas in the liver it reversed the GCA induced inhibition of both 11 -HSD1 and 11 -HSD2. Thus, catecholamines may be one of the mediators opposing inhibition of 11 -HSD by GCA during stress.

Original languageEnglish
Pages (from-to)544-547
Number of pages4
JournalPakistan Journal of Nutrition
Volume5
Issue number6
DOIs
Publication statusPublished - 2006

Fingerprint

isoprenaline
11-beta-Hydroxysteroid Dehydrogenases
glycyrrhizin
Glycyrrhizic Acid
hydroxysteroid dehydrogenases
Isoproterenol
propranolol
catecholamines
hypothalamus
Propranolol
Hypothalamus
Catecholamines
rats
kidneys
cortisone
Kidney
liver
Liver
Cortisone
tap water

Keywords

  • 11 -HSD
  • Glycyrrhizic acid
  • Isoprenaline

ASJC Scopus subject areas

  • Food Science
  • Nutrition and Dietetics
  • Public Health, Environmental and Occupational Health

Cite this

Isoprenaline reverses glycyrrhizic acid - Induced inhibition of 11-hydroxysteroid dehydrogenase bioactivity. / Suhaimi, Farihah; Mohamad, Nabishah; Abdul Kadir, Khalid Bin.

In: Pakistan Journal of Nutrition, Vol. 5, No. 6, 2006, p. 544-547.

Research output: Contribution to journalArticle

Suhaimi, Farihah ; Mohamad, Nabishah ; Abdul Kadir, Khalid Bin. / Isoprenaline reverses glycyrrhizic acid - Induced inhibition of 11-hydroxysteroid dehydrogenase bioactivity. In: Pakistan Journal of Nutrition. 2006 ; Vol. 5, No. 6. pp. 544-547.
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