IsomiRs have functional importance

Geok Chin Tan, Nicholas Dibb

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Since the inception of deep sequencing, isomiRs are consistently observed to be produced by most miRNA genes in a variety of cell types. IsomiRs appear as a variation in length from the canonical sequence annotated in miRBase, due to an addition or deletion of one or more nucleotides at the 5’ or 3’ ends or both. As the seed sequence is located at the 5’ end of the microRNA, the target mRNA will be theoretically different. Therefore, 5’isomiRs might potentially target a new set mRNA compared to their canonical counterpart. This article gives an overview of investigations that explored the functional potential of isomiRs such as their ability to incorporate into Argonaute protein, the differential expression of isomiRs in various tissue types and cell lines, and the differences of mRNA targets between isomiR and its canonical microRNA. In addition, this article provides a brief introduction of RNA sponges as a potential way to inhibit isomiRs.

Original languageEnglish
Pages (from-to)73-81
Number of pages9
JournalMalaysian Journal of Pathology
Volume37
Issue number2
Publication statusPublished - 1 Aug 2015

Fingerprint

MicroRNAs
Messenger RNA
Argonaute Proteins
High-Throughput Nucleotide Sequencing
Porifera
Seeds
Nucleotides
RNA
Cell Line
Genes

Keywords

  • IsomiRs
  • Messenger RNA
  • microRNA
  • Molecular
  • Target prediction

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Cell Biology
  • Histology

Cite this

IsomiRs have functional importance. / Tan, Geok Chin; Dibb, Nicholas.

In: Malaysian Journal of Pathology, Vol. 37, No. 2, 01.08.2015, p. 73-81.

Research output: Contribution to journalArticle

Tan, GC & Dibb, N 2015, 'IsomiRs have functional importance', Malaysian Journal of Pathology, vol. 37, no. 2, pp. 73-81.
Tan, Geok Chin ; Dibb, Nicholas. / IsomiRs have functional importance. In: Malaysian Journal of Pathology. 2015 ; Vol. 37, No. 2. pp. 73-81.
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