Isolation rearing induces recognition memory deficits accompanied by cytoskeletal alterations in rat hippocampus

M. Bianchi, K. F C Fone, Norazrina Azmi, C. A. Heidbreder, J. J. Hagan, C. A. Marsden

Research output: Contribution to journalArticle

118 Citations (Scopus)

Abstract

Social isolation from weaning affects hippocampal structure and function in the rat. The intrinsic dynamic instability of the cytoskeletal microtubular system is essential for neuronal development and organization. Accordingly, the present paper investigated the effects of social isolation on hippocampal levels of α-tubulin isoforms associated with microtubule dynamics, the dendritic marker MAP-2 and alterations in locomotor activity and recognition memory. Male Lister Hooded rats (postnatal day 25-28) were housed either in groups or singly (isolated animals) for 30 days. Locomotor activity in a novel arena and novel object recognition were monitored in activity boxes. The hippocampus was dissected out 18 h after the novel object recognition task. Levels of α-tubulin isoforms and MAP-2 were analysed using Western blots. The experiments were conducted in duplicate, using two batches of rats obtained from different suppliers. Isolated animals were hyperactive and showed recognition memory deficits in the novel object recognition task. These behavioural alterations were accompanied by specific alterations in hippocampal α-tubulin isoforms and decreased MAP-2 expression. The results confirm that rearing rats in isolation produces hyperactivity and cognitive deficits. The behavioural alterations were accompanied by hippocampal cytoskeletal changes consistent with microtubule stabilization, and by decreased MAP-2 expression. These findings are indicative of an abnormal development of synaptic connections and/or reductions in neuronal cell number. The developmental structural abnormalities in the hippocampus may contribute to the cognitive impairments which result from isolation rearing in rats.

Original languageEnglish
Pages (from-to)2894-2902
Number of pages9
JournalEuropean Journal of Neuroscience
Volume24
Issue number10
DOIs
Publication statusPublished - Dec 2006
Externally publishedYes

Fingerprint

Memory Disorders
Hippocampus
Tubulin
Social Isolation
Protein Isoforms
Locomotion
Microtubules
Weaning
Cell Count
Western Blotting
Recognition (Psychology)
Organizations

Keywords

  • α-tubulin isoforms
  • Early stress
  • Microtubules
  • Novel object recognition

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Isolation rearing induces recognition memory deficits accompanied by cytoskeletal alterations in rat hippocampus. / Bianchi, M.; Fone, K. F C; Azmi, Norazrina; Heidbreder, C. A.; Hagan, J. J.; Marsden, C. A.

In: European Journal of Neuroscience, Vol. 24, No. 10, 12.2006, p. 2894-2902.

Research output: Contribution to journalArticle

Bianchi, M. ; Fone, K. F C ; Azmi, Norazrina ; Heidbreder, C. A. ; Hagan, J. J. ; Marsden, C. A. / Isolation rearing induces recognition memory deficits accompanied by cytoskeletal alterations in rat hippocampus. In: European Journal of Neuroscience. 2006 ; Vol. 24, No. 10. pp. 2894-2902.
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