Pemencilan sebatian botrien dari Hypoxylon rickii dan pengenalpastian pengekodan gen

Translated title of the contribution: Isolation of two botryanes from Hypoxylon rickii and identification of the encoding genes

Afnani Alwi, Andi Rifki Rosandy, Farah Diba Abu Bakar, Rozida Mohd. Khalid

Research output: Contribution to journalArticle

Abstract

10-oxodehydrobotrydial (1) and 4β-Acetoxy-9β,10β,15α-trihydroxyprobotrydial (2) have been isolated from Hypoxylon rickii mycelia extract. Compound 2 is probably an intermediate of botrydial, which is a known sesquiterpene phytotoxin. Cluster Omega and Artemis software analysis suggested that HRT6, a possible botrydial-like gene cluster with four genes shows more than 50% similarities compared to Botrytis cinerea botrydial gene cluster, BcBOT. A comparison between proposed 4β-Acetoxy-9β,10β,15α- trihydroxyprobotrydial (2) producing pathway and BcBOT proposed pathway shows compatible function of each gene prediction. Further confirmation using RNA gene knock-outs is on-going.

Original languageMalay
Pages (from-to)1389-1398
Number of pages10
JournalMalaysian Journal of Analytical Sciences
Volume21
Issue number6
DOIs
Publication statusPublished - 1 Dec 2017

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Gene encoding
Genes
Sesquiterpenes
RNA
botrydial

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Pemencilan sebatian botrien dari Hypoxylon rickii dan pengenalpastian pengekodan gen. / Alwi, Afnani; Rosandy, Andi Rifki; Abu Bakar, Farah Diba; Mohd. Khalid, Rozida.

In: Malaysian Journal of Analytical Sciences, Vol. 21, No. 6, 01.12.2017, p. 1389-1398.

Research output: Contribution to journalArticle

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AB - 10-oxodehydrobotrydial (1) and 4β-Acetoxy-9β,10β,15α-trihydroxyprobotrydial (2) have been isolated from Hypoxylon rickii mycelia extract. Compound 2 is probably an intermediate of botrydial, which is a known sesquiterpene phytotoxin. Cluster Omega and Artemis software analysis suggested that HRT6, a possible botrydial-like gene cluster with four genes shows more than 50% similarities compared to Botrytis cinerea botrydial gene cluster, BcBOT. A comparison between proposed 4β-Acetoxy-9β,10β,15α- trihydroxyprobotrydial (2) producing pathway and BcBOT proposed pathway shows compatible function of each gene prediction. Further confirmation using RNA gene knock-outs is on-going.

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