Isolation of the metallothionein gene from white mustard, brassica rapa var parachinensis

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

This paper describes the method of isolation of the metallothionein gene from white mustard also known as Brassica rapa var parachinensis. White mustard is one of the main vegetables planted and consumed by human beings worldwide. This vegetable is usually grown on a large scale and requires a good irrigation system. Tolerance of the white mustard plant to heavy metal pollution has caught the interest of many researchers motivating them to try to isolate the gene that is believed to be able to detoxify toxic elements in the soil. And this gene is known as metallothionein. In the current study, the DNA genome from the white mustard was isolated, followed with the amplification process of the the metallothionein gene with the polymerase chain reaction (PCR) using a specific primer namely MTFS. The optimum annealing temperature was 57°C and a bright 230 bp band was seen on 1% agarose gel. The PCR product was then purified for further use in sequencing and cloning activities. The sequencing results showed successful isolation of the metallothionein gene from the DNA genome with significant match of the nucleotide sequences to those from other organisms which also carried the metallothionein gene. This is also proof that the white mustard plant carries the class II metallothionein gene because its cystein residues were distributed all over its sequence. The gene was successfully cloned using the pDrive cloning vector into the QIAGEN EZ competent cell be stored in the glycerol stock for further use.

Original languageEnglish
Pages (from-to)68-73
Number of pages6
JournalAdvances in Environmental Biology
Volume4
Issue number1
Publication statusPublished - Jan 2010

Fingerprint

Sinapis
Sinapis alba subsp. alba
Brassica rapa
metallothionein
Metallothionein
gene
Genes
genes
Mustard Plant
Vegetables
polymerase chain reaction
vegetable
Genome
genome
vegetables
Polymerase Chain Reaction
Genetic Vectors
MHC Class II Genes
genetic vectors
Poisons

Keywords

  • Annealing temperature
  • Gene
  • Heavy metals
  • Metallothionein
  • Sequence

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Environmental Science(all)

Cite this

@article{7fdf4605b89f434c97605bd8ac485709,
title = "Isolation of the metallothionein gene from white mustard, brassica rapa var parachinensis",
abstract = "This paper describes the method of isolation of the metallothionein gene from white mustard also known as Brassica rapa var parachinensis. White mustard is one of the main vegetables planted and consumed by human beings worldwide. This vegetable is usually grown on a large scale and requires a good irrigation system. Tolerance of the white mustard plant to heavy metal pollution has caught the interest of many researchers motivating them to try to isolate the gene that is believed to be able to detoxify toxic elements in the soil. And this gene is known as metallothionein. In the current study, the DNA genome from the white mustard was isolated, followed with the amplification process of the the metallothionein gene with the polymerase chain reaction (PCR) using a specific primer namely MTFS. The optimum annealing temperature was 57°C and a bright 230 bp band was seen on 1{\%} agarose gel. The PCR product was then purified for further use in sequencing and cloning activities. The sequencing results showed successful isolation of the metallothionein gene from the DNA genome with significant match of the nucleotide sequences to those from other organisms which also carried the metallothionein gene. This is also proof that the white mustard plant carries the class II metallothionein gene because its cystein residues were distributed all over its sequence. The gene was successfully cloned using the pDrive cloning vector into the QIAGEN EZ competent cell be stored in the glycerol stock for further use.",
keywords = "Annealing temperature, Gene, Heavy metals, Metallothionein, Sequence",
author = "{Mohd Fahmi}, {A. B.} and Sidik, {Nik Marzuki} and {Che Mohd. Zain}, {Che Radziah} and Khairiah Jusoh and Roslina, {M. Y.} and Ismail Sahid",
year = "2010",
month = "1",
language = "English",
volume = "4",
pages = "68--73",
journal = "Advances in Environmental Biology",
issn = "1995-0756",
publisher = "American-Eurasian Network for Scientific Information",
number = "1",

}

TY - JOUR

T1 - Isolation of the metallothionein gene from white mustard, brassica rapa var parachinensis

AU - Mohd Fahmi, A. B.

AU - Sidik, Nik Marzuki

AU - Che Mohd. Zain, Che Radziah

AU - Jusoh, Khairiah

AU - Roslina, M. Y.

AU - Sahid, Ismail

PY - 2010/1

Y1 - 2010/1

N2 - This paper describes the method of isolation of the metallothionein gene from white mustard also known as Brassica rapa var parachinensis. White mustard is one of the main vegetables planted and consumed by human beings worldwide. This vegetable is usually grown on a large scale and requires a good irrigation system. Tolerance of the white mustard plant to heavy metal pollution has caught the interest of many researchers motivating them to try to isolate the gene that is believed to be able to detoxify toxic elements in the soil. And this gene is known as metallothionein. In the current study, the DNA genome from the white mustard was isolated, followed with the amplification process of the the metallothionein gene with the polymerase chain reaction (PCR) using a specific primer namely MTFS. The optimum annealing temperature was 57°C and a bright 230 bp band was seen on 1% agarose gel. The PCR product was then purified for further use in sequencing and cloning activities. The sequencing results showed successful isolation of the metallothionein gene from the DNA genome with significant match of the nucleotide sequences to those from other organisms which also carried the metallothionein gene. This is also proof that the white mustard plant carries the class II metallothionein gene because its cystein residues were distributed all over its sequence. The gene was successfully cloned using the pDrive cloning vector into the QIAGEN EZ competent cell be stored in the glycerol stock for further use.

AB - This paper describes the method of isolation of the metallothionein gene from white mustard also known as Brassica rapa var parachinensis. White mustard is one of the main vegetables planted and consumed by human beings worldwide. This vegetable is usually grown on a large scale and requires a good irrigation system. Tolerance of the white mustard plant to heavy metal pollution has caught the interest of many researchers motivating them to try to isolate the gene that is believed to be able to detoxify toxic elements in the soil. And this gene is known as metallothionein. In the current study, the DNA genome from the white mustard was isolated, followed with the amplification process of the the metallothionein gene with the polymerase chain reaction (PCR) using a specific primer namely MTFS. The optimum annealing temperature was 57°C and a bright 230 bp band was seen on 1% agarose gel. The PCR product was then purified for further use in sequencing and cloning activities. The sequencing results showed successful isolation of the metallothionein gene from the DNA genome with significant match of the nucleotide sequences to those from other organisms which also carried the metallothionein gene. This is also proof that the white mustard plant carries the class II metallothionein gene because its cystein residues were distributed all over its sequence. The gene was successfully cloned using the pDrive cloning vector into the QIAGEN EZ competent cell be stored in the glycerol stock for further use.

KW - Annealing temperature

KW - Gene

KW - Heavy metals

KW - Metallothionein

KW - Sequence

UR - http://www.scopus.com/inward/record.url?scp=77955632937&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77955632937&partnerID=8YFLogxK

M3 - Article

VL - 4

SP - 68

EP - 73

JO - Advances in Environmental Biology

JF - Advances in Environmental Biology

SN - 1995-0756

IS - 1

ER -