Isolation of terpenoids from the stem of ficus aurantiaca griff and their effects on reactive oxygen species production and chemotactic activity of neutrophils

Shukranul Mawa, Ibrahim Jantan, Khairana Husain

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Three new triterpenoids; namely 28,28,30-trihydroxylupeol (1); 3,21,21,26-tetrahydroxylanostanoic acid (2) and dehydroxybetulinic acid (3) and seven known compounds; i.e., taraxerone (4); taraxerol (5); ethyl palmitate (6); herniarin (7); stigmasterol (8); ursolic acid (9) and acetyl ursolic acid (10) were isolated from the stem of Ficus aurantiaca Griff. The structures of the compounds were established by spectroscopic techniques. The compounds were evaluated for their inhibitory effects on polymorphonuclear leukocyte (PMN) chemotaxis by using the Boyden chamber technique and on human whole blood and neutrophil reactive oxygen species (ROS) production by using a luminol-based chemiluminescence assay. Among the compounds tested, compounds 1-4, 6 and 9 exhibited strong inhibition of PMN migration towards the chemoattractant N-formyl-methionyl-leucyl-phenylalanine (fMLP) with IC50 values of 6.8; 2.8; 2.5; 4.1; 3.7 and 3.6 μM, respectively, comparable to that of the positive control ibuprofen (6.7 μM). Compounds 2-4, 6, 7 and 9 exhibited strong inhibition of ROS production of PMNs with IC50 values of 0.9; 0.9; 1.3; 1.1; 0.5 and 0.8 μM, respectively, which were lower than that of aspirin (9.4 μM). The bioactive compounds might be potential lead molecules for the development of new immunomodulatory agents to modulate the innate immune response of phagocytes.

Original languageEnglish
Article number9
JournalMolecules
Volume21
Issue number1
DOIs
Publication statusPublished - 1 Jan 2016

Fingerprint

neutrophils
Ficus
Terpenes
stems
Reactive Oxygen Species
isolation
Neutrophils
Stigmasterol
N-Formylmethionine Leucyl-Phenylalanine
Luminol
leukocytes
acids
Inhibitory Concentration 50
Acids
Chemiluminescence
Ibuprofen
Chemotactic Factors
oxygen
Leukocyte Chemotaxis
Aspirin

Keywords

  • Chemotaxis
  • Ficus aurantiaca griff
  • Immunomodulatory
  • Neutrophils
  • Reactive oxygen species
  • Terpenoids

ASJC Scopus subject areas

  • Medicine(all)
  • Organic Chemistry

Cite this

Isolation of terpenoids from the stem of ficus aurantiaca griff and their effects on reactive oxygen species production and chemotactic activity of neutrophils. / Mawa, Shukranul; Jantan, Ibrahim; Husain, Khairana.

In: Molecules, Vol. 21, No. 1, 9, 01.01.2016.

Research output: Contribution to journalArticle

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