Isolation of phytochemicals from Cordia rothii (Boraginaceae) and evaluation of their immunomodulatory properties

Sadiqa Firdous, Kehkashan Khan, Sadia Zikr-Ur-Rehman, Zulfiqar Ali, Samreen Soomro, Viqar U. Ahmad, Munawwer Rasheed, Osman Mesaik Mohammed Ahmed Hassan, Shaheen Faizi

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

n-Hexane, EtOAc, and BuOH fractions obtained from methanol extract of leaves of Cordia rothii Roem and Schult, yielded 1-octacosanol (1), β-sitosterol (2), stigmast-5-en-3-O-β-D-glucoside (3), (2S,1′S,2′S,3′ R,7′Z)-N -1′-(O-β-D-glucopyranosyl)methyl-2′,3′-dihydroxy-heptadec-7′-enyl-2-hydroxytetracosane-amide (4), methyl 2-hydroxy-3-(4′-hydroxy)-phenyl propionate (5), (2R)-(4-hydroxyphenyl)lactic acid (6), syringaresinol mono-β-D-glucoside (7), 6-hydroxy-3-oxo-α-ionol 9-O-β-D-glucopyranoside (8), staphylionoside D (9), and 3-(3′5′dimethoxy-4′-O-β -D-glucopyranosyl-phenyl)-prop-2E-en-1-ol (10). To the best of our knowledge, all compounds except 2 are reported for the first time from this source. Norterpenoid, lignan, cerebroside, and megastigmane are also reported for the first time. Immunomodulatory activity was evaluated using oxidative burst; phytohaemagglutinin (PHA) stimulated T-cell proliferation and nitric oxide (NO) assays. EtOAc fraction exhibited significant inhibitory activity against ROS with IC50 value of 29.4 ± 2.8 μg/mL. n-Hexane fraction exhibited very strong suppressive effect on PHA stimulated T-cell proliferation with IC50 value of 85% inhibition. Compound 7 exhibited significant inhibitory activity (IC50 = 11.3 and 18.0 on PMN and whole blood respectively) on zymosan activated ROS generation. Eight sub-fractions showed potent inhibitory activity on the NO with >60% inhibition. These results suggest that compounds from C. rothii could be potential inhibitor for the mediators involved in innate and adaptive immune responses, and potential anti-inflammatory agents.

Original languageEnglish
Pages (from-to)51-55
Number of pages5
JournalRecords of Natural Products
Volume8
Issue number1
Publication statusPublished - 2014
Externally publishedYes

Fingerprint

Cordia
Boraginaceae
T-cells
Glucosides
Cell proliferation
Phytochemicals
Phytohemagglutinins
phytopharmaceuticals
Inhibitory Concentration 50
inhibitory concentration 50
Nitric Oxide
phytohemagglutinin
Cerebrosides
Butylated Hydroxytoluene
hexane
Lignans
Zymosan
glucosides
nitric oxide
cell proliferation

Keywords

  • Cordia rothii
  • Immunomodulatory activity
  • Natural constituents

ASJC Scopus subject areas

  • Organic Chemistry
  • Drug Discovery
  • Pharmacology
  • Plant Science

Cite this

Firdous, S., Khan, K., Zikr-Ur-Rehman, S., Ali, Z., Soomro, S., Ahmad, V. U., ... Faizi, S. (2014). Isolation of phytochemicals from Cordia rothii (Boraginaceae) and evaluation of their immunomodulatory properties. Records of Natural Products, 8(1), 51-55.

Isolation of phytochemicals from Cordia rothii (Boraginaceae) and evaluation of their immunomodulatory properties. / Firdous, Sadiqa; Khan, Kehkashan; Zikr-Ur-Rehman, Sadia; Ali, Zulfiqar; Soomro, Samreen; Ahmad, Viqar U.; Rasheed, Munawwer; Mohammed Ahmed Hassan, Osman Mesaik; Faizi, Shaheen.

In: Records of Natural Products, Vol. 8, No. 1, 2014, p. 51-55.

Research output: Contribution to journalArticle

Firdous, S, Khan, K, Zikr-Ur-Rehman, S, Ali, Z, Soomro, S, Ahmad, VU, Rasheed, M, Mohammed Ahmed Hassan, OM & Faizi, S 2014, 'Isolation of phytochemicals from Cordia rothii (Boraginaceae) and evaluation of their immunomodulatory properties', Records of Natural Products, vol. 8, no. 1, pp. 51-55.
Firdous, Sadiqa ; Khan, Kehkashan ; Zikr-Ur-Rehman, Sadia ; Ali, Zulfiqar ; Soomro, Samreen ; Ahmad, Viqar U. ; Rasheed, Munawwer ; Mohammed Ahmed Hassan, Osman Mesaik ; Faizi, Shaheen. / Isolation of phytochemicals from Cordia rothii (Boraginaceae) and evaluation of their immunomodulatory properties. In: Records of Natural Products. 2014 ; Vol. 8, No. 1. pp. 51-55.
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AU - Ali, Zulfiqar

AU - Soomro, Samreen

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AU - Mohammed Ahmed Hassan, Osman Mesaik

AU - Faizi, Shaheen

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