Isolation of food-borne pathogen bacteriophages from retail food and environmental sewage

T. Y. Thung, B. M.F. Siti Norshafawatie, J. M.K.J.K. Premarathne, W. S. Chang, Y. Y. Loo, C. H. Kuan, C. Y. New, A. Ubong, O. S.B. Ramzi, N. A. Mahyudin, Dayang Fredalina Basri, W. M.R. Che Wan Jasimah, R. Son

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Bacteriophages are the viruses of bacteria and are widely distributed in the biosphere, exhibiting dramatic manifestations both in liquid cultures and on solid media. In this study, bacteriophages were isolated from different types of food (beef, chicken meats, cucumber, lettuce, clam, cockles and shrimp) and sewage samples using 6 reference pathogen strains (Salmonella Enteritidis, Salmonella Typhimurium, Campylobacter jejuni, Vibrio parahaemolyticus, Listeria monocytogenes and Escherichia coli). A total of 29 bacteriophage isolates were obtained and further examined for titer via agar overlay assay. The titers were determined within the range of 108 to 1011 PFU/mL. Our results showed that diverse of bacteriophages are naturally present in a variety of foods.

Original languageEnglish
Pages (from-to)450-454
Number of pages5
JournalInternational Food Research Journal
Volume24
Issue number1
Publication statusPublished - 2017

Fingerprint

food retailing
food pathogens
Sewage
sewage
bacteriophages
Bacteriophages
Food
Cardiidae
Vibrio parahaemolyticus
Cucumis sativus
Lettuce
Salmonella enteritidis
Campylobacter jejuni
Bivalvia
chicken meat
Listeria monocytogenes
Salmonella Enteritidis
Salmonella typhimurium
clams
Salmonella Typhimurium

Keywords

  • Agar overlay assay
  • Bacteriophage
  • Food-borne pathogen
  • Isolation

ASJC Scopus subject areas

  • Food Science

Cite this

Thung, T. Y., Siti Norshafawatie, B. M. F., Premarathne, J. M. K. J. K., Chang, W. S., Loo, Y. Y., Kuan, C. H., ... Son, R. (2017). Isolation of food-borne pathogen bacteriophages from retail food and environmental sewage. International Food Research Journal, 24(1), 450-454.

Isolation of food-borne pathogen bacteriophages from retail food and environmental sewage. / Thung, T. Y.; Siti Norshafawatie, B. M.F.; Premarathne, J. M.K.J.K.; Chang, W. S.; Loo, Y. Y.; Kuan, C. H.; New, C. Y.; Ubong, A.; Ramzi, O. S.B.; Mahyudin, N. A.; Basri, Dayang Fredalina; Che Wan Jasimah, W. M.R.; Son, R.

In: International Food Research Journal, Vol. 24, No. 1, 2017, p. 450-454.

Research output: Contribution to journalArticle

Thung, TY, Siti Norshafawatie, BMF, Premarathne, JMKJK, Chang, WS, Loo, YY, Kuan, CH, New, CY, Ubong, A, Ramzi, OSB, Mahyudin, NA, Basri, DF, Che Wan Jasimah, WMR & Son, R 2017, 'Isolation of food-borne pathogen bacteriophages from retail food and environmental sewage', International Food Research Journal, vol. 24, no. 1, pp. 450-454.
Thung TY, Siti Norshafawatie BMF, Premarathne JMKJK, Chang WS, Loo YY, Kuan CH et al. Isolation of food-borne pathogen bacteriophages from retail food and environmental sewage. International Food Research Journal. 2017;24(1):450-454.
Thung, T. Y. ; Siti Norshafawatie, B. M.F. ; Premarathne, J. M.K.J.K. ; Chang, W. S. ; Loo, Y. Y. ; Kuan, C. H. ; New, C. Y. ; Ubong, A. ; Ramzi, O. S.B. ; Mahyudin, N. A. ; Basri, Dayang Fredalina ; Che Wan Jasimah, W. M.R. ; Son, R. / Isolation of food-borne pathogen bacteriophages from retail food and environmental sewage. In: International Food Research Journal. 2017 ; Vol. 24, No. 1. pp. 450-454.
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