Isolation of dinucleotide microsatellite markers in commercially threatened Malaysian Mahseer, Tor tambroides.

S. Bhassu, Yosni Bakar, Z. A. Rashid

Research output: Contribution to journalArticle

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Abstract

Seven single locus dinucleotide microsatellite markers were developed to characterize an economically important sport fish and food fish in Malaysia and in Southeast Asia. They were obtained by using a rapid method namely the 5' anchored PCR enrichment protocol. The specific primers were designed to flank the repeat sequences and these were subsequently used to characterize 120 unrelated fish from Malaysia and 30 fishes from Indonesia. The number of alleles per locus ranged from 2 (SYKVJ1-11) to 6 (SYKVJ1-4) while the levels of heterozygosity ranged from 0.0472 (SYKVJ1-11) to 0.7745 (SYKVJ1-2).

Original languageEnglish
Pages (from-to)1145-1147
Number of pages3
JournalGenetika
Volume44
Issue number8
Publication statusPublished - Aug 2008

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Microsatellite Repeats
Fishes
Malaysia
Southeastern Asia
Indonesia
Sports
Alleles
Food
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Genetics

Cite this

Isolation of dinucleotide microsatellite markers in commercially threatened Malaysian Mahseer, Tor tambroides. / Bhassu, S.; Bakar, Yosni; Rashid, Z. A.

In: Genetika, Vol. 44, No. 8, 08.2008, p. 1145-1147.

Research output: Contribution to journalArticle

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