Isolation of acanthamoeba spp. from contact lens paraphernalia

Mohamed Kamel Abdul Ghani, Saleha Abdul Majid, Noradillah Samseh Abdullah, Anisah Nordin, Yusof Suboh, Noraina Abd Rahim, Haliza Abdul Mutalib, Norazah Ahmad

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Introduction: Acanthamoeba spp. are ubiquitous free-living protozoa that are widely distributed in all types of environment throughout the world. Acanthamoeba sp. is the causative agent of two diseases; keratitis and granulomatous amoebic encephalitis. Contaminated contact lenses and corneal lesions are the major risk factors in causing Acanthamoeba keratitis. Objective: The main objective of this study is to isolate Acanthamoeba sp. from contact lens paraphernalia. Materials and Methods: One hundred and seventy five swabs from contact lens paraphernalia were obtained, consisting of 66 swabs of contact lenses, 52 swabs of contact lens storage cases and 57 samples of contact lens disinfecting solutions. Filtration and culture techniques were used to isolate the organism using standard methods. Results: This study successfully isolated Acanthamoeba sp. from contact lenses and contact lens storage cases at 10.6% and 13.5% respectively. However no Acanthamoeba sp. was isolated from the contact lens disinfecting solution. Many of those contact lens wearers whose contact lens paraphernalia were positive for Acanthamoeba showed a deviation from contact lens wear and care procedures recommended by the lens manufacturer and health professionals. Conclusion: The findings that contact lens paraphernalia harbours Acanthamoeba is particularly worrying as the number of cases is increasing. Contact lens wearers should be educated regarding proper hygienic care of their contact lenses.

Original languageEnglish
Pages (from-to)66-68
Number of pages3
JournalInternational Medical Journal
Volume20
Issue number1
Publication statusPublished - Feb 2013

Fingerprint

Acanthamoeba
Contact Lenses
Contact Lens Solutions
Acanthamoeba Keratitis
Culture Techniques
Keratitis
Encephalitis
Lenses

Keywords

  • Acanthamoeba
  • Cleaning solutions
  • Contact lens
  • Storage cases

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Abdul Ghani, M. K., Majid, S. A., Abdullah, N. S., Nordin, A., Suboh, Y., Rahim, N. A., ... Ahmad, N. (2013). Isolation of acanthamoeba spp. from contact lens paraphernalia. International Medical Journal, 20(1), 66-68.

Isolation of acanthamoeba spp. from contact lens paraphernalia. / Abdul Ghani, Mohamed Kamel; Majid, Saleha Abdul; Abdullah, Noradillah Samseh; Nordin, Anisah; Suboh, Yusof; Rahim, Noraina Abd; Abdul Mutalib, Haliza; Ahmad, Norazah.

In: International Medical Journal, Vol. 20, No. 1, 02.2013, p. 66-68.

Research output: Contribution to journalArticle

Abdul Ghani, MK, Majid, SA, Abdullah, NS, Nordin, A, Suboh, Y, Rahim, NA, Abdul Mutalib, H & Ahmad, N 2013, 'Isolation of acanthamoeba spp. from contact lens paraphernalia', International Medical Journal, vol. 20, no. 1, pp. 66-68.
Abdul Ghani, Mohamed Kamel ; Majid, Saleha Abdul ; Abdullah, Noradillah Samseh ; Nordin, Anisah ; Suboh, Yusof ; Rahim, Noraina Abd ; Abdul Mutalib, Haliza ; Ahmad, Norazah. / Isolation of acanthamoeba spp. from contact lens paraphernalia. In: International Medical Journal. 2013 ; Vol. 20, No. 1. pp. 66-68.
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