Isolation of Acanthamoeba sp. from conjunctival sac of healthy individuals using swab.

Anisah Nordin, H. Amal, Mohamed Kamel Abdul Ghani, S. Yusof, A. R. Noraina, Norhayati Moktar

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Is Acanthamoeba sp. normally found in the eyes? A study was carried out to establish the possibility of Acanthamoeba sp. as a part of the normal conjunctival flora. Conjunctiva swabbing were carried out in 286 healthy Orang Asli school children using sterile cotton swab. The swab was then inoculated onto non-nutrient agar (NN-A). Heat killed Escherichia coli that was used as food source for the growth of the amoebae was pipetted onto and away from the smear. The plates were incubated at 30 degrees C and examined daily using an inverted microscope for 14 days. Morphology of the trophozoites and cysts of the amoebae were used as the taxonomic criteria for identification. Positive-controls and negative-controls were done to check for the consistency of the technique used and monitoring of contamination respectively. None of the conjunctiva swab cultured was positive for Acanthamoeba sp. This finding may indicate that Acanthamoeba sp. is not part of normal conjunctival flora or conjunctiva swab is an insensitive technique to isolate the organism. However, a more extensive research is needed to investigate these possibilities.

Original languageEnglish
Pages (from-to)11-14
Number of pages4
JournalTropical biomedicine.
Volume22
Issue number1
Publication statusPublished - Jun 2005

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Acanthamoeba
Lacrimal Apparatus
Conjunctiva
Amoeba
Trophozoites
Agar
Cysts
Hot Temperature
Escherichia coli
Food
Growth
Research

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Isolation of Acanthamoeba sp. from conjunctival sac of healthy individuals using swab. / Nordin, Anisah; Amal, H.; Abdul Ghani, Mohamed Kamel; Yusof, S.; Noraina, A. R.; Moktar, Norhayati.

In: Tropical biomedicine., Vol. 22, No. 1, 06.2005, p. 11-14.

Research output: Contribution to journalArticle

Nordin, Anisah ; Amal, H. ; Abdul Ghani, Mohamed Kamel ; Yusof, S. ; Noraina, A. R. ; Moktar, Norhayati. / Isolation of Acanthamoeba sp. from conjunctival sac of healthy individuals using swab. In: Tropical biomedicine. 2005 ; Vol. 22, No. 1. pp. 11-14.
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