Isolation of a Clostridium acetobutylicum strain and characterization of its fermentation performance on agricultural wastes

Najeeb Kaid Nasser Al-Shorgani, Mohd Hafez Mohd Isa, Wan Mohtar Wan Yusoff, Mohd. Sahaid Kalil, Aidil Abdul Hamid

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

A new solvent-producing Clostridium has been isolated from soil used in intensive rice cultivation. The 16S rRNA analysis of the isolate indicates that it is closely related to Clostridium acetobutylicum, with a sequence identity of 96%. The new isolate, named C. acetobutylicum YM1, produces biobutanol from multiple carbon sources, including glucose, fructose, xylose, arabinose, glycerol, lactose, cellobiose, mannitol, maltose, galactose, sucrose and mannose. This isolate can also utilize polysaccharides such as starch and carboxylmethyl cellulose (CMC) for the production of biobutanol. The ability of isolate YM1 to produce biobutanol from agro-industrial wastes was also evaluated for rice bran, de-oiled rice bran, palm oil mill effluent and palm kernel cake. The highest concentration of biobutanol (7.27 g/L) was obtained from the fermentation medium containing 2% (w/v) fructose, with a total acetone-butanol-ethanol (ABE) concentration of 10.23 g/L. The ability of isolate YM1 to produce biobutanol from various carbon sources and agro-wastes indicates the promise of the use of this isolate for the production of biobutanol, a renewable energy resource, from readily available renewable feedstocks.

Original languageEnglish
Article number7081
Pages (from-to)459-465
Number of pages7
JournalRenewable Energy
Volume86
DOIs
Publication statusPublished - 1 Feb 2016

Fingerprint

Clostridium
Agricultural wastes
Fructose
Fermentation
Maltose
Renewable energy resources
Carbon
Xylose
Palm oil
Industrial wastes
Sugar (sucrose)
Polysaccharides
Starch
Butenes
Glycerol
Acetone
Feedstocks
Glucose
Effluents
Cellulose

Keywords

  • Biobutanol
  • Characterization
  • Clostridium
  • Isolation
  • Renewable energy

ASJC Scopus subject areas

  • Renewable Energy, Sustainability and the Environment

Cite this

Isolation of a Clostridium acetobutylicum strain and characterization of its fermentation performance on agricultural wastes. / Al-Shorgani, Najeeb Kaid Nasser; Isa, Mohd Hafez Mohd; Wan Yusoff, Wan Mohtar; Kalil, Mohd. Sahaid; Abdul Hamid, Aidil.

In: Renewable Energy, Vol. 86, 7081, 01.02.2016, p. 459-465.

Research output: Contribution to journalArticle

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