Isolation of 15 polymorphic microsatellite loci in Acacia hybrid (Acacia mangium x Acacia auriculiformis)

C. H. Ng, S. C. Koh, S. L. Lee, K. K S Ng, A. Mark, M. Norwati, R Wickneswari V Ratnam

Research output: Contribution to journalArticle

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Abstract

This study reports the isolation of 15 microsatellites in Acacia hybrid (Acacia mangium x Acacia auriculiformis) based on the 5′ anchored polymerase chain reaction technique. Polymorphism of these loci was evaluated in a sample of 24 hybrid individuals. The level of polymorphism ranged from two to eight alleles with an observed heterozygosity ranging from 0.083 to 0.875. These loci were also characterized in both the parental species. The number of alleles ranged from two to six for both A. mangium and A. auriculiformis with the observed heterozygosity values ranging from 0.167 to 0.625 and 0.042 to 0.458 respectively. Five of these loci demonstrated Mendelian inheritance in a segregating F1 population; four presented a distorted ratio and the remaining six did not segregate in progenies as they were homozygous in both parents.

Original languageEnglish
Pages (from-to)572-575
Number of pages4
JournalMolecular Ecology Notes
Volume5
Issue number3
DOIs
Publication statusPublished - Sep 2005

Fingerprint

Acacia auriculiformis
Acacia
Acacia mangium
Polymorphism
heterozygosity
Microsatellite Repeats
allele
polymorphism
microsatellite repeats
loci
Polymerase chain reaction
Alleles
genetic polymorphism
polymerase chain reaction
alleles
Mendelian inheritance
Polymerase Chain Reaction
Population
sampling
methodology

Keywords

  • Acacia auriculiformis
  • Acacia hybrid
  • Acacia mangium
  • Microsatellite
  • Tropical tree

ASJC Scopus subject areas

  • Ecology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry

Cite this

Isolation of 15 polymorphic microsatellite loci in Acacia hybrid (Acacia mangium x Acacia auriculiformis). / Ng, C. H.; Koh, S. C.; Lee, S. L.; Ng, K. K S; Mark, A.; Norwati, M.; V Ratnam, R Wickneswari.

In: Molecular Ecology Notes, Vol. 5, No. 3, 09.2005, p. 572-575.

Research output: Contribution to journalArticle

Ng, C. H. ; Koh, S. C. ; Lee, S. L. ; Ng, K. K S ; Mark, A. ; Norwati, M. ; V Ratnam, R Wickneswari. / Isolation of 15 polymorphic microsatellite loci in Acacia hybrid (Acacia mangium x Acacia auriculiformis). In: Molecular Ecology Notes. 2005 ; Vol. 5, No. 3. pp. 572-575.
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