Isolation, detection of virulence genes, antibiotic resistance genes, plasmid profile, and molecular typing among Vibrio parahaemolyticus isolated in Malaysian seawater from recreational beaches and fish

Orooba Meteab Faja, Ali Abd Sharad, Khansa Mohammed Younis, Merriam Ghadhanfar Alwan, Basima Jasim Mohammed, Asmat Ahmad

Research output: Contribution to journalArticle

Abstract

Background and Aim: Despite the importance of the global emergence of Vibrio parahaemolyticus infections worldwide, there has been scanty information on its occurrence in Malaysian seawaters and fish. This study aimed to determine the occurrence of V. parahaemolyticus isolates using polymerase chain reaction targeted at toxin operon gene, thermostable direct hemolysin (tdh), and tdh-related hemolysin genes and to determine antibiotic resistance pattern, genes, and plasmid profile of V. parahaemolyticus from Malaysian seawaters and fish. Materials and Methods: Samples were collected from four recreational beaches in Malaysia (Port Klang; Bachok; Port Dickson; and Mersing). Thiosulfate-citrate-bile salts-sucrose (TCBS) agar and chromogenic Vibrio agar were used for isolation and identification. Colonies with yellow color on TCBS and green color on chromogenic vibrio (CV) agar were considered to be V. parahaemolyticus and they were subjected to biochemical tests. All V. parahaemolyticus isolates were further subjected to identification using seven specific gene markers. Results: Seventy-three Vibrio isolates were recovered. Only one gene tdh from seawater isolates of Vibrio has high virulence gene percentage (95.23%). Two genes alkaline serine protease (asp) and (tdh) had high percentage of virulence (83.87% and 80.64%, respectively) from fish. Comparatively, fish isolates have a higher virulence percentage compared to seawater isolates. Only gene streptomycin resistance B (strB) from seawater had 100% of the resistance genes. All isolates were multi-antibiotic resistant. Seventeen antibiotic resistance patterns were observed. The isolates had plasmids of varying sizes ranging from 2.7 kb to 42.4 kb. Dendrogram based on antibiotic resistance patterns of V. parahaemolyticus isolates discriminated the isolates into three clusters. Conclusion: This study demonstrated the occurrence of pathogenic, multi-antibiotic-resistant V. parahaemolyticus strains in Malaysian coastal waters and fish, and this could constitute potential public health risks.

Original languageEnglish
Pages (from-to)1140-1149
Number of pages10
JournalVeterinary World
Volume12
Issue number7
DOIs
Publication statusPublished - 1 Jul 2019

Fingerprint

Vibrio parahaemolyticus
Molecular Typing
Seawater
Microbial Drug Resistance
antibiotic resistance
beaches
Virulence
plasmids
Fishes
Plasmids
virulence
seawater
hemolysins
Vibrio
fish
Genes
genes
thiosulfates
Agar
bile salts

Keywords

  • Antibiotic resistance genes
  • Plasmid profile
  • Vibrio parahaemolyticus
  • Virulence genes

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Isolation, detection of virulence genes, antibiotic resistance genes, plasmid profile, and molecular typing among Vibrio parahaemolyticus isolated in Malaysian seawater from recreational beaches and fish. / Faja, Orooba Meteab; Sharad, Ali Abd; Younis, Khansa Mohammed; Alwan, Merriam Ghadhanfar; Mohammed, Basima Jasim; Ahmad, Asmat.

In: Veterinary World, Vol. 12, No. 7, 01.07.2019, p. 1140-1149.

Research output: Contribution to journalArticle

Faja, Orooba Meteab ; Sharad, Ali Abd ; Younis, Khansa Mohammed ; Alwan, Merriam Ghadhanfar ; Mohammed, Basima Jasim ; Ahmad, Asmat. / Isolation, detection of virulence genes, antibiotic resistance genes, plasmid profile, and molecular typing among Vibrio parahaemolyticus isolated in Malaysian seawater from recreational beaches and fish. In: Veterinary World. 2019 ; Vol. 12, No. 7. pp. 1140-1149.
@article{9ec1d09291e64042baab0f59099a70b5,
title = "Isolation, detection of virulence genes, antibiotic resistance genes, plasmid profile, and molecular typing among Vibrio parahaemolyticus isolated in Malaysian seawater from recreational beaches and fish",
abstract = "Background and Aim: Despite the importance of the global emergence of Vibrio parahaemolyticus infections worldwide, there has been scanty information on its occurrence in Malaysian seawaters and fish. This study aimed to determine the occurrence of V. parahaemolyticus isolates using polymerase chain reaction targeted at toxin operon gene, thermostable direct hemolysin (tdh), and tdh-related hemolysin genes and to determine antibiotic resistance pattern, genes, and plasmid profile of V. parahaemolyticus from Malaysian seawaters and fish. Materials and Methods: Samples were collected from four recreational beaches in Malaysia (Port Klang; Bachok; Port Dickson; and Mersing). Thiosulfate-citrate-bile salts-sucrose (TCBS) agar and chromogenic Vibrio agar were used for isolation and identification. Colonies with yellow color on TCBS and green color on chromogenic vibrio (CV) agar were considered to be V. parahaemolyticus and they were subjected to biochemical tests. All V. parahaemolyticus isolates were further subjected to identification using seven specific gene markers. Results: Seventy-three Vibrio isolates were recovered. Only one gene tdh from seawater isolates of Vibrio has high virulence gene percentage (95.23{\%}). Two genes alkaline serine protease (asp) and (tdh) had high percentage of virulence (83.87{\%} and 80.64{\%}, respectively) from fish. Comparatively, fish isolates have a higher virulence percentage compared to seawater isolates. Only gene streptomycin resistance B (strB) from seawater had 100{\%} of the resistance genes. All isolates were multi-antibiotic resistant. Seventeen antibiotic resistance patterns were observed. The isolates had plasmids of varying sizes ranging from 2.7 kb to 42.4 kb. Dendrogram based on antibiotic resistance patterns of V. parahaemolyticus isolates discriminated the isolates into three clusters. Conclusion: This study demonstrated the occurrence of pathogenic, multi-antibiotic-resistant V. parahaemolyticus strains in Malaysian coastal waters and fish, and this could constitute potential public health risks.",
keywords = "Antibiotic resistance genes, Plasmid profile, Vibrio parahaemolyticus, Virulence genes",
author = "Faja, {Orooba Meteab} and Sharad, {Ali Abd} and Younis, {Khansa Mohammed} and Alwan, {Merriam Ghadhanfar} and Mohammed, {Basima Jasim} and Asmat Ahmad",
year = "2019",
month = "7",
day = "1",
doi = "10.14202/vetworld.2019.1140-1149",
language = "English",
volume = "12",
pages = "1140--1149",
journal = "Veterinary World",
issn = "0972-8988",
publisher = "Veterinary World",
number = "7",

}

TY - JOUR

T1 - Isolation, detection of virulence genes, antibiotic resistance genes, plasmid profile, and molecular typing among Vibrio parahaemolyticus isolated in Malaysian seawater from recreational beaches and fish

AU - Faja, Orooba Meteab

AU - Sharad, Ali Abd

AU - Younis, Khansa Mohammed

AU - Alwan, Merriam Ghadhanfar

AU - Mohammed, Basima Jasim

AU - Ahmad, Asmat

PY - 2019/7/1

Y1 - 2019/7/1

N2 - Background and Aim: Despite the importance of the global emergence of Vibrio parahaemolyticus infections worldwide, there has been scanty information on its occurrence in Malaysian seawaters and fish. This study aimed to determine the occurrence of V. parahaemolyticus isolates using polymerase chain reaction targeted at toxin operon gene, thermostable direct hemolysin (tdh), and tdh-related hemolysin genes and to determine antibiotic resistance pattern, genes, and plasmid profile of V. parahaemolyticus from Malaysian seawaters and fish. Materials and Methods: Samples were collected from four recreational beaches in Malaysia (Port Klang; Bachok; Port Dickson; and Mersing). Thiosulfate-citrate-bile salts-sucrose (TCBS) agar and chromogenic Vibrio agar were used for isolation and identification. Colonies with yellow color on TCBS and green color on chromogenic vibrio (CV) agar were considered to be V. parahaemolyticus and they were subjected to biochemical tests. All V. parahaemolyticus isolates were further subjected to identification using seven specific gene markers. Results: Seventy-three Vibrio isolates were recovered. Only one gene tdh from seawater isolates of Vibrio has high virulence gene percentage (95.23%). Two genes alkaline serine protease (asp) and (tdh) had high percentage of virulence (83.87% and 80.64%, respectively) from fish. Comparatively, fish isolates have a higher virulence percentage compared to seawater isolates. Only gene streptomycin resistance B (strB) from seawater had 100% of the resistance genes. All isolates were multi-antibiotic resistant. Seventeen antibiotic resistance patterns were observed. The isolates had plasmids of varying sizes ranging from 2.7 kb to 42.4 kb. Dendrogram based on antibiotic resistance patterns of V. parahaemolyticus isolates discriminated the isolates into three clusters. Conclusion: This study demonstrated the occurrence of pathogenic, multi-antibiotic-resistant V. parahaemolyticus strains in Malaysian coastal waters and fish, and this could constitute potential public health risks.

AB - Background and Aim: Despite the importance of the global emergence of Vibrio parahaemolyticus infections worldwide, there has been scanty information on its occurrence in Malaysian seawaters and fish. This study aimed to determine the occurrence of V. parahaemolyticus isolates using polymerase chain reaction targeted at toxin operon gene, thermostable direct hemolysin (tdh), and tdh-related hemolysin genes and to determine antibiotic resistance pattern, genes, and plasmid profile of V. parahaemolyticus from Malaysian seawaters and fish. Materials and Methods: Samples were collected from four recreational beaches in Malaysia (Port Klang; Bachok; Port Dickson; and Mersing). Thiosulfate-citrate-bile salts-sucrose (TCBS) agar and chromogenic Vibrio agar were used for isolation and identification. Colonies with yellow color on TCBS and green color on chromogenic vibrio (CV) agar were considered to be V. parahaemolyticus and they were subjected to biochemical tests. All V. parahaemolyticus isolates were further subjected to identification using seven specific gene markers. Results: Seventy-three Vibrio isolates were recovered. Only one gene tdh from seawater isolates of Vibrio has high virulence gene percentage (95.23%). Two genes alkaline serine protease (asp) and (tdh) had high percentage of virulence (83.87% and 80.64%, respectively) from fish. Comparatively, fish isolates have a higher virulence percentage compared to seawater isolates. Only gene streptomycin resistance B (strB) from seawater had 100% of the resistance genes. All isolates were multi-antibiotic resistant. Seventeen antibiotic resistance patterns were observed. The isolates had plasmids of varying sizes ranging from 2.7 kb to 42.4 kb. Dendrogram based on antibiotic resistance patterns of V. parahaemolyticus isolates discriminated the isolates into three clusters. Conclusion: This study demonstrated the occurrence of pathogenic, multi-antibiotic-resistant V. parahaemolyticus strains in Malaysian coastal waters and fish, and this could constitute potential public health risks.

KW - Antibiotic resistance genes

KW - Plasmid profile

KW - Vibrio parahaemolyticus

KW - Virulence genes

UR - http://www.scopus.com/inward/record.url?scp=85071375475&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85071375475&partnerID=8YFLogxK

U2 - 10.14202/vetworld.2019.1140-1149

DO - 10.14202/vetworld.2019.1140-1149

M3 - Article

AN - SCOPUS:85071375475

VL - 12

SP - 1140

EP - 1149

JO - Veterinary World

JF - Veterinary World

SN - 0972-8988

IS - 7

ER -