Isolation by culture and PCR identification of Lipl32 gene of pathogenic Leptospira spp. In wild rats of Kuala Lumpur

Ibrahim Latifah, A. Abdul Halim, Ms Rahmat, M. Faten Nadia, Z. Edmond Ubil, Asmah Hamid, I. Shafariatul Akmar, Mathieu Picardeau, O. Siti Haslina, M. Amal Nasir

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Background: A study was conducted to confirm the status of rats as the carrier of pathogenic leptospira in Kuala Lumpur, Malaysia. Method: A total of 140 urine samples were collected from trapped rats. These samples were cultured in EMJH enriched media and 18 of these samples (12.9%) were found to be positive when observed under x40 by dark field microscope. Genomic DNA was extracted from all the 18 native isolates for PCR. Result: All the 18 isolates generated the expected 786 base pair band when the set of primers known to amplify LipL32 gene were utilized. These results showed that the primers were suitable to be used for the identification of pathogenic leptospira from the 18 rat samples. Conclusion: The sequencing of the PCR products and BLAST analysis performed on each representative isolates confirmed the pathogenic status of all these native isolates as the LipL32 gene was detected in all the Leptospira isolates. This indicates that the rats are carriers of the pathogenic leptospira in the study area, and therefore are of public health importance.

Original languageEnglish
Pages (from-to)161-166
Number of pages6
JournalMalaysian Journal of Pathology
Volume39
Issue number2
Publication statusPublished - 1 Aug 2017

Fingerprint

Leptospira
Polymerase Chain Reaction
Genes
Malaysia
Base Pairing
Public Health
Urine
DNA

Keywords

  • Leptospira
  • LipL32 gene
  • Pathogenic
  • Polymerase chain reaction
  • Urine isolates
  • Wild rats

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology
  • Cell Biology

Cite this

Latifah, I., Abdul Halim, A., Rahmat, M., Faten Nadia, M., Ubil, Z. E., Hamid, A., ... Nasir, M. A. (2017). Isolation by culture and PCR identification of Lipl32 gene of pathogenic Leptospira spp. In wild rats of Kuala Lumpur. Malaysian Journal of Pathology, 39(2), 161-166.

Isolation by culture and PCR identification of Lipl32 gene of pathogenic Leptospira spp. In wild rats of Kuala Lumpur. / Latifah, Ibrahim; Abdul Halim, A.; Rahmat, Ms; Faten Nadia, M.; Ubil, Z. Edmond; Hamid, Asmah; Shafariatul Akmar, I.; Picardeau, Mathieu; Siti Haslina, O.; Nasir, M. Amal.

In: Malaysian Journal of Pathology, Vol. 39, No. 2, 01.08.2017, p. 161-166.

Research output: Contribution to journalArticle

Latifah, I, Abdul Halim, A, Rahmat, M, Faten Nadia, M, Ubil, ZE, Hamid, A, Shafariatul Akmar, I, Picardeau, M, Siti Haslina, O & Nasir, MA 2017, 'Isolation by culture and PCR identification of Lipl32 gene of pathogenic Leptospira spp. In wild rats of Kuala Lumpur', Malaysian Journal of Pathology, vol. 39, no. 2, pp. 161-166.
Latifah, Ibrahim ; Abdul Halim, A. ; Rahmat, Ms ; Faten Nadia, M. ; Ubil, Z. Edmond ; Hamid, Asmah ; Shafariatul Akmar, I. ; Picardeau, Mathieu ; Siti Haslina, O. ; Nasir, M. Amal. / Isolation by culture and PCR identification of Lipl32 gene of pathogenic Leptospira spp. In wild rats of Kuala Lumpur. In: Malaysian Journal of Pathology. 2017 ; Vol. 39, No. 2. pp. 161-166.
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