Isolation and regeneration protoplast of an oil palm pathogen, Ganoderma boninense

Liza Isaac Irene, Farah Diba Abu Bakar, Abu Seman Idris, Abdul Munir Abd. Murad

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Ganoderma boninense is a known cause for basal stem rot (BSR) in oil palm. Thus, to curb the infection towards oil palm, the establishment of protoplast isolation and regeneration protocol is crucial to be studied. This will provide information on the functional genes especially those which leads towards infection and pathogenicity. In this study, a method was outlined to isolated protoplast in G. boninense by manipulating parameters such as mycelium age, concentration of lysing enzyme, and duration of mycelia incubation in lytic solution. The results shows that from 0.1 g of wet weight mycelia, the highest protoplast yield obtained was 5.5 × 108 protoplast/ml using 5th day old culture in a lytic mixture containing 2.0 % of lysing enzyme incubated for 4 hours at 30 °C with agitation of 80-100 rpm. The highest percentage of protoplast regeneration obtained from this study was 0.2 % using CYM medium supplemented with 0.6 M sorbitol. To date, this is the first report of protoplast isolation and regeneration for this phytopathogen.

Original languageEnglish
Title of host publication2015 UKM FST Postgraduate Colloquium: Proceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2015 Postgraduate Colloquium
PublisherAmerican Institute of Physics Inc.
Volume1678
ISBN (Electronic)9780735413252
DOIs
Publication statusPublished - 25 Sep 2015
Event2015 Postgraduate Colloquium of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology, UKM FST 2015 - Selangor, Malaysia
Duration: 15 Apr 201516 Apr 2015

Other

Other2015 Postgraduate Colloquium of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology, UKM FST 2015
CountryMalaysia
CitySelangor
Period15/4/1516/4/15

Fingerprint

protoplasts
pathogens
regeneration
isolation
oils
infectious diseases
enzymes
agitation
stems
genes
causes

ASJC Scopus subject areas

  • Physics and Astronomy(all)

Cite this

Irene, L. I., Abu Bakar, F. D., Idris, A. S., & Abd. Murad, A. M. (2015). Isolation and regeneration protoplast of an oil palm pathogen, Ganoderma boninense. In 2015 UKM FST Postgraduate Colloquium: Proceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2015 Postgraduate Colloquium (Vol. 1678). [030003] American Institute of Physics Inc.. https://doi.org/10.1063/1.4931224

Isolation and regeneration protoplast of an oil palm pathogen, Ganoderma boninense. / Irene, Liza Isaac; Abu Bakar, Farah Diba; Idris, Abu Seman; Abd. Murad, Abdul Munir.

2015 UKM FST Postgraduate Colloquium: Proceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2015 Postgraduate Colloquium. Vol. 1678 American Institute of Physics Inc., 2015. 030003.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Irene, LI, Abu Bakar, FD, Idris, AS & Abd. Murad, AM 2015, Isolation and regeneration protoplast of an oil palm pathogen, Ganoderma boninense. in 2015 UKM FST Postgraduate Colloquium: Proceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2015 Postgraduate Colloquium. vol. 1678, 030003, American Institute of Physics Inc., 2015 Postgraduate Colloquium of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology, UKM FST 2015, Selangor, Malaysia, 15/4/15. https://doi.org/10.1063/1.4931224
Irene LI, Abu Bakar FD, Idris AS, Abd. Murad AM. Isolation and regeneration protoplast of an oil palm pathogen, Ganoderma boninense. In 2015 UKM FST Postgraduate Colloquium: Proceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2015 Postgraduate Colloquium. Vol. 1678. American Institute of Physics Inc. 2015. 030003 https://doi.org/10.1063/1.4931224
Irene, Liza Isaac ; Abu Bakar, Farah Diba ; Idris, Abu Seman ; Abd. Murad, Abdul Munir. / Isolation and regeneration protoplast of an oil palm pathogen, Ganoderma boninense. 2015 UKM FST Postgraduate Colloquium: Proceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2015 Postgraduate Colloquium. Vol. 1678 American Institute of Physics Inc., 2015.
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