Isolation and molecular analysis of cDNA encoding a partial endoglucanase gene from Aspergillus terreus SUK-1

Nik Marzuki Sidik, Adzni Sharifuddin Shaiful, Omar Othman, Jalil Abdul Kader Abdul, Sahidan Senafi

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

We report here the isolation and sequence analysis of a cDNA fragment for an endoglucanase gene from Aspergillus terreus SUK-1. A pair of primers was designed based on conserved regions of endoglucanase gene. Amplification of A. terreus SUK-1 cDNA using the primers produced a DNA band of 642 bp. The cDNA fragment was purified and cloned into pCRæ-II-TOPO plasmid vector. The cDNA insert was designated as SA2 and sequenced. Analysis of the SA2 sequence showed a high degree of identity towards endoglucanase gene sequences from A. aculeatus, A kawachii and Talaromyces emersonii. While, the putative amino acid sequence of SA2 showed 74 % identity towards endoglucanase protein from Thermoascus aurantiacus and 69 % identity towards endoglucanase protein from A. kawachii, A. niger, A. aculeatus and A. nidulans. A 66 % identity between SA2 putative amino acid sequence and A. oryzae endoglucanase protein sequence was also observed. Therefore, we concluded that SA2 codes for a putative endoglucanase gene of A. terreus SUK-1.

Original languageEnglish
Pages (from-to)31-35
Number of pages5
JournalSains Malaysiana
Volume35
Issue number1
Publication statusPublished - Jul 2006

Fingerprint

Aspergillus terreus
endo-1,4-beta-glucanase
genes
amino acid sequences
sequence analysis
plasmid vectors
Oryza
proteins
nucleotide sequences
DNA

Keywords

  • Aspergillus terreus SUK-1
  • Cellulase
  • CMCase
  • Endoglucanase
  • Gene

ASJC Scopus subject areas

  • General

Cite this

Sidik, N. M., Shaiful, A. S., Othman, O., Abdul, J. A. K., & Senafi, S. (2006). Isolation and molecular analysis of cDNA encoding a partial endoglucanase gene from Aspergillus terreus SUK-1. Sains Malaysiana, 35(1), 31-35.

Isolation and molecular analysis of cDNA encoding a partial endoglucanase gene from Aspergillus terreus SUK-1. / Sidik, Nik Marzuki; Shaiful, Adzni Sharifuddin; Othman, Omar; Abdul, Jalil Abdul Kader; Senafi, Sahidan.

In: Sains Malaysiana, Vol. 35, No. 1, 07.2006, p. 31-35.

Research output: Contribution to journalArticle

Sidik, NM, Shaiful, AS, Othman, O, Abdul, JAK & Senafi, S 2006, 'Isolation and molecular analysis of cDNA encoding a partial endoglucanase gene from Aspergillus terreus SUK-1', Sains Malaysiana, vol. 35, no. 1, pp. 31-35.
Sidik, Nik Marzuki ; Shaiful, Adzni Sharifuddin ; Othman, Omar ; Abdul, Jalil Abdul Kader ; Senafi, Sahidan. / Isolation and molecular analysis of cDNA encoding a partial endoglucanase gene from Aspergillus terreus SUK-1. In: Sains Malaysiana. 2006 ; Vol. 35, No. 1. pp. 31-35.
@article{71d433c584eb4486b875cb228076a8fb,
title = "Isolation and molecular analysis of cDNA encoding a partial endoglucanase gene from Aspergillus terreus SUK-1",
abstract = "We report here the isolation and sequence analysis of a cDNA fragment for an endoglucanase gene from Aspergillus terreus SUK-1. A pair of primers was designed based on conserved regions of endoglucanase gene. Amplification of A. terreus SUK-1 cDNA using the primers produced a DNA band of 642 bp. The cDNA fragment was purified and cloned into pCR{\ae}-II-TOPO plasmid vector. The cDNA insert was designated as SA2 and sequenced. Analysis of the SA2 sequence showed a high degree of identity towards endoglucanase gene sequences from A. aculeatus, A kawachii and Talaromyces emersonii. While, the putative amino acid sequence of SA2 showed 74 {\%} identity towards endoglucanase protein from Thermoascus aurantiacus and 69 {\%} identity towards endoglucanase protein from A. kawachii, A. niger, A. aculeatus and A. nidulans. A 66 {\%} identity between SA2 putative amino acid sequence and A. oryzae endoglucanase protein sequence was also observed. Therefore, we concluded that SA2 codes for a putative endoglucanase gene of A. terreus SUK-1.",
keywords = "Aspergillus terreus SUK-1, Cellulase, CMCase, Endoglucanase, Gene",
author = "Sidik, {Nik Marzuki} and Shaiful, {Adzni Sharifuddin} and Omar Othman and Abdul, {Jalil Abdul Kader} and Sahidan Senafi",
year = "2006",
month = "7",
language = "English",
volume = "35",
pages = "31--35",
journal = "Sains Malaysiana",
issn = "0126-6039",
publisher = "Penerbit Universiti Kebangsaan Malaysia",
number = "1",

}

TY - JOUR

T1 - Isolation and molecular analysis of cDNA encoding a partial endoglucanase gene from Aspergillus terreus SUK-1

AU - Sidik, Nik Marzuki

AU - Shaiful, Adzni Sharifuddin

AU - Othman, Omar

AU - Abdul, Jalil Abdul Kader

AU - Senafi, Sahidan

PY - 2006/7

Y1 - 2006/7

N2 - We report here the isolation and sequence analysis of a cDNA fragment for an endoglucanase gene from Aspergillus terreus SUK-1. A pair of primers was designed based on conserved regions of endoglucanase gene. Amplification of A. terreus SUK-1 cDNA using the primers produced a DNA band of 642 bp. The cDNA fragment was purified and cloned into pCRæ-II-TOPO plasmid vector. The cDNA insert was designated as SA2 and sequenced. Analysis of the SA2 sequence showed a high degree of identity towards endoglucanase gene sequences from A. aculeatus, A kawachii and Talaromyces emersonii. While, the putative amino acid sequence of SA2 showed 74 % identity towards endoglucanase protein from Thermoascus aurantiacus and 69 % identity towards endoglucanase protein from A. kawachii, A. niger, A. aculeatus and A. nidulans. A 66 % identity between SA2 putative amino acid sequence and A. oryzae endoglucanase protein sequence was also observed. Therefore, we concluded that SA2 codes for a putative endoglucanase gene of A. terreus SUK-1.

AB - We report here the isolation and sequence analysis of a cDNA fragment for an endoglucanase gene from Aspergillus terreus SUK-1. A pair of primers was designed based on conserved regions of endoglucanase gene. Amplification of A. terreus SUK-1 cDNA using the primers produced a DNA band of 642 bp. The cDNA fragment was purified and cloned into pCRæ-II-TOPO plasmid vector. The cDNA insert was designated as SA2 and sequenced. Analysis of the SA2 sequence showed a high degree of identity towards endoglucanase gene sequences from A. aculeatus, A kawachii and Talaromyces emersonii. While, the putative amino acid sequence of SA2 showed 74 % identity towards endoglucanase protein from Thermoascus aurantiacus and 69 % identity towards endoglucanase protein from A. kawachii, A. niger, A. aculeatus and A. nidulans. A 66 % identity between SA2 putative amino acid sequence and A. oryzae endoglucanase protein sequence was also observed. Therefore, we concluded that SA2 codes for a putative endoglucanase gene of A. terreus SUK-1.

KW - Aspergillus terreus SUK-1

KW - Cellulase

KW - CMCase

KW - Endoglucanase

KW - Gene

UR - http://www.scopus.com/inward/record.url?scp=34548762974&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34548762974&partnerID=8YFLogxK

M3 - Article

VL - 35

SP - 31

EP - 35

JO - Sains Malaysiana

JF - Sains Malaysiana

SN - 0126-6039

IS - 1

ER -