Isolation and In silico characterization of plant defensin, Ctd1, From the tropical forage legume (Clitoria ternatea L.)

Noriha Mat Amin, Bunawan Hamidun, Norliza Tendot Abu Bakar

Research output: Contribution to journalArticle

Abstract

A plant defensin, ctd1, was isolated from the tropical forage legume, Butterfly Pea (Clitoria ternatea L.). The full-length cDNA consists of a 228 bp open reading frame with 75 amino acids, of which the N-terminal 28 amino acids constitute the signal peptide and 47 amino acids constitute the mature peptide. A characteristic of this protein is eight cysteine residues forming four disulphide bridges (Cys14-Cys35, Cys20-Cys41, Cys24-Cys43 and Cys3-Cys47) to stabilize the mature peptide. The phylogenetic based on the mature peptide was inferred using neighbor-joining method found Ctd1 clustered with other Phaseoleae species. The γ-core motif identified in this protein highlights the important molecules of Ctd1 peptides and hence appears as a suitable candidate for deployment in transgenic crops for disease resistance.

Original languageEnglish
Pages (from-to)1009-1014
Number of pages6
JournalAdvances in Environmental Biology
Volume8
Issue number4
Publication statusPublished - 2014
Externally publishedYes

Fingerprint

Clitoria
Clitoria ternatea
Defensins
forage legumes
Fabaceae
Computer Simulation
peptide
forage
peptides
Peptides
Amino Acids
amino acid
amino acids
Butterflies
Disease Resistance
Peas
Protein Sorting Signals
signal peptide
sulfides
protein

Keywords

  • Clitoria ternatea L.
  • Ctd1
  • Cysteine rich peptides
  • Defensin

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Environmental Science(all)

Cite this

Isolation and In silico characterization of plant defensin, Ctd1, From the tropical forage legume (Clitoria ternatea L.). / Amin, Noriha Mat; Hamidun, Bunawan; Bakar, Norliza Tendot Abu.

In: Advances in Environmental Biology, Vol. 8, No. 4, 2014, p. 1009-1014.

Research output: Contribution to journalArticle

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