Isolation and identification of putative mesenchymal stem cells from bone marrow.

P. L. Mok, Leong Chooi Fun, S. K. Cheong

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Mesenchymal stem cells are pluripotent progenitor cells found in bone marrow that have the capacity of differentiating into bone, cartilage, tendon, fat, muscle, and early progenitors of neural cells under certain conditions. It has also been shown that they support haematopoiesis in culture. We have successfully isolated mesenchymal stem cells from a bone marrow sample of an anaemic patient by using DMEM supplemented with 10% of FBS. They could be distinguished from other cells by their tendency to adhere to tissue culture plastic. The adherent time was about three to seven days. Microscopically, the cultured cells showed morphology resembling fibroblast and they divided actively. Early passage cultures were heterogenous and contained four morphologically distinct cell types; long spindle-shaped cells, star-shaped cells, petal-shaped cells and large flattened granular cells with vacuoles. Identification of mesenchymal stem cells was carried out by cytochemical analysis and immunophenotyping by flow cytometry. Identification of these cells is vital as they have properties that appear to make them ideal candidates for studying differentiation and make them suitable for cellular and gene therapy.

Original languageEnglish
Pages (from-to)121-127
Number of pages7
JournalThe Malaysian journal of pathology
Volume25
Issue number2
Publication statusPublished - 2003

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Mesenchymal Stromal Cells
Bone Marrow
Stem Cells
Immunophenotyping
Hematopoiesis
Vacuoles
Genetic Therapy
Tendons
Plastics
Cartilage
Cultured Cells
Flow Cytometry
Fibroblasts
Fats
Bone and Bones
Muscles

Cite this

Isolation and identification of putative mesenchymal stem cells from bone marrow. / Mok, P. L.; Chooi Fun, Leong; Cheong, S. K.

In: The Malaysian journal of pathology, Vol. 25, No. 2, 2003, p. 121-127.

Research output: Contribution to journalArticle

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