Isolation and identification of microsatellite repeat motifs from the Epinephelus fuscoguttatus genome

Mohd Azinuddin Ahmad Mokhtar, Syarul Nataqain Baharum, Normah Mohd. Noor, Vijay Kumar

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Epinephelus fuscoguttatus belongs to one of the largest serranidae fish family. Genetic information regarding existing fish populations in the wild is crucial for the conservation, particularly since the species is listed under the IUCD Red List due to intense fishing. Microsatellites of E. fuscoguttatus were isolated using streptavidin-biotin enrichment method. In total, 378 microsatellites were identified and characterized. Of these 378 total microsatellites, 46 (12.2%) were mononucleotides, 175 (46.3%) were dinucleotides, 109 (28.8%) were trinucleotides, 36 (9.5%) were tetranucloetides, 7 (1.9%) were pentanucleotides, 4 (1.1%) were hexanucleotides and 1 (0.3%) was a heptanucleotide. The most abundant microsatellite present in E. fuscoguttatus was the dinucleotide motif, (AC) n.

Original languageEnglish
Pages (from-to)8553-8557
Number of pages5
JournalAfrican Journal of Biotechnology
Volume10
Issue number43
Publication statusPublished - Aug 2010

Fingerprint

Epinephelus fuscoguttatus
Microsatellite Repeats
Genome
microsatellite repeats
genome
Fishes
Bass
Serranidae
streptavidin
Streptavidin
biotin
Biotin
fish
Population

Keywords

  • Diversity
  • Enrichment
  • Epinephelus fuscoguttatus
  • Microsatellite

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Genetics
  • Molecular Biology
  • Agronomy and Crop Science

Cite this

Isolation and identification of microsatellite repeat motifs from the Epinephelus fuscoguttatus genome. / Mokhtar, Mohd Azinuddin Ahmad; Baharum, Syarul Nataqain; Mohd. Noor, Normah; Kumar, Vijay.

In: African Journal of Biotechnology, Vol. 10, No. 43, 08.2010, p. 8553-8557.

Research output: Contribution to journalArticle

Mokhtar, Mohd Azinuddin Ahmad ; Baharum, Syarul Nataqain ; Mohd. Noor, Normah ; Kumar, Vijay. / Isolation and identification of microsatellite repeat motifs from the Epinephelus fuscoguttatus genome. In: African Journal of Biotechnology. 2010 ; Vol. 10, No. 43. pp. 8553-8557.
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