Isolation and identification of marine sulphate-reducing bacteria, Desulfovibrio sp. and Citrobacter freundii from Pasir Gudang, Malaysia

Fathul Karim Sahrani, Zaharah Ibrahim, Adibah Yahya, Madzlan Aziz

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

In the attempt to isolate indigenous marine sulphate-reducing bacteria (SRB) from coastal samples, we obtained some swarm forming bacteria. The isolates were screening using Starkey's medium and detection of main groups of SRB were carried out using commercially kits (SRB BART kits, Droycon Bioconcepts Inc., Canada). From the growth characteristic, only two isolates were strongly suggested as marine SRB. Based on the 16S rRNA gene sequence analysis these SRB were closely related and could be designated as Desulfovibrio sp. and Citrobacter freundii, with the highest sequence similarity of 98% and 93%, respectively.

Original languageEnglish
Pages (from-to)365-371
Number of pages7
JournalSains Malaysiana
Volume37
Issue number4
Publication statusPublished - Dec 2008

Fingerprint

sulfate-reducing bacterium
bacterium
gene

Keywords

  • 16S rRNA sequence
  • SRB BART kits
  • Sulphate-reducing bacteria

ASJC Scopus subject areas

  • General

Cite this

Isolation and identification of marine sulphate-reducing bacteria, Desulfovibrio sp. and Citrobacter freundii from Pasir Gudang, Malaysia. / Sahrani, Fathul Karim; Ibrahim, Zaharah; Yahya, Adibah; Aziz, Madzlan.

In: Sains Malaysiana, Vol. 37, No. 4, 12.2008, p. 365-371.

Research output: Contribution to journalArticle

@article{bc7bd3ae73a14ab099f5f2fc6fbd97dc,
title = "Isolation and identification of marine sulphate-reducing bacteria, Desulfovibrio sp. and Citrobacter freundii from Pasir Gudang, Malaysia",
abstract = "In the attempt to isolate indigenous marine sulphate-reducing bacteria (SRB) from coastal samples, we obtained some swarm forming bacteria. The isolates were screening using Starkey's medium and detection of main groups of SRB were carried out using commercially kits (SRB BART kits, Droycon Bioconcepts Inc., Canada). From the growth characteristic, only two isolates were strongly suggested as marine SRB. Based on the 16S rRNA gene sequence analysis these SRB were closely related and could be designated as Desulfovibrio sp. and Citrobacter freundii, with the highest sequence similarity of 98{\%} and 93{\%}, respectively.",
keywords = "16S rRNA sequence, SRB BART kits, Sulphate-reducing bacteria",
author = "Sahrani, {Fathul Karim} and Zaharah Ibrahim and Adibah Yahya and Madzlan Aziz",
year = "2008",
month = "12",
language = "English",
volume = "37",
pages = "365--371",
journal = "Sains Malaysiana",
issn = "0126-6039",
publisher = "Penerbit Universiti Kebangsaan Malaysia",
number = "4",

}

TY - JOUR

T1 - Isolation and identification of marine sulphate-reducing bacteria, Desulfovibrio sp. and Citrobacter freundii from Pasir Gudang, Malaysia

AU - Sahrani, Fathul Karim

AU - Ibrahim, Zaharah

AU - Yahya, Adibah

AU - Aziz, Madzlan

PY - 2008/12

Y1 - 2008/12

N2 - In the attempt to isolate indigenous marine sulphate-reducing bacteria (SRB) from coastal samples, we obtained some swarm forming bacteria. The isolates were screening using Starkey's medium and detection of main groups of SRB were carried out using commercially kits (SRB BART kits, Droycon Bioconcepts Inc., Canada). From the growth characteristic, only two isolates were strongly suggested as marine SRB. Based on the 16S rRNA gene sequence analysis these SRB were closely related and could be designated as Desulfovibrio sp. and Citrobacter freundii, with the highest sequence similarity of 98% and 93%, respectively.

AB - In the attempt to isolate indigenous marine sulphate-reducing bacteria (SRB) from coastal samples, we obtained some swarm forming bacteria. The isolates were screening using Starkey's medium and detection of main groups of SRB were carried out using commercially kits (SRB BART kits, Droycon Bioconcepts Inc., Canada). From the growth characteristic, only two isolates were strongly suggested as marine SRB. Based on the 16S rRNA gene sequence analysis these SRB were closely related and could be designated as Desulfovibrio sp. and Citrobacter freundii, with the highest sequence similarity of 98% and 93%, respectively.

KW - 16S rRNA sequence

KW - SRB BART kits

KW - Sulphate-reducing bacteria

UR - http://www.scopus.com/inward/record.url?scp=56849103281&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=56849103281&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:56849103281

VL - 37

SP - 365

EP - 371

JO - Sains Malaysiana

JF - Sains Malaysiana

SN - 0126-6039

IS - 4

ER -