Isolation and identification of local bacteria endophyte and screening of its antimicrobial property against pathogenic bacteria and fungi

Ahmad Syairazie Ibrahim Fikri, Irman Abdul Rahman, Norefrina Shafinaz Md Nor, Ainon Hamzah

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Citations (Scopus)

Abstract

Endophytes are organisms, often fungi and bacteria that live in living plant cells. These organisms reside in the living tissues of the host plant in a variety of relationships, ranging from symbiotic to slightly pathogenic. The endophytes may produce a plethora of substances that have potential to be used in modern medicine, agriculture and industry. The aims of this study are to isolate, identify and screening antimicrobial activity of bacterial endophytes. The endophytes were isolated using nutrient agar, incubated at 37°C for 48 hours. Identification of the isolates were done based on morphological characteristics, biochemical tests and 16S rDNA molecular analysis. Disk diffusion method was used to screen for antimicrobial activity of metabolites from endophytes against pathogenic bacteria. Screening for antifungal activity of selected endophytes was done using dual culture method againts pathogenic fungi followed by Kirby-Bauer method. Results showed endophytes designated as B2c and B7b have positive antimicrobial activity. The metabolites from isolate B2c showed antimicrobial activity against pathogenic bacteria methicillin-resistant Staphylococcus aureus (MRSA), Staphylococcus aureus and Staphylococcus epidermis, while isolate B7b have positive activities againts MRSA, S. aureus and Pseudomonas aeruginosa. Isolates B2c displayed antifungal activity against Fusarium oxysporum, Fusarium solani, Phytophthora palmivora and Colletotrichum gloeosporioides. Identification using biochemical tests and 16S rDNA sequences identified isolate B2c as Pseudomonas resinovorans with 97% homology and isolate B7b as Bacillus subtilis with 98% homology.

Original languageEnglish
Title of host publication2017 UKM FST Postgraduate Colloquium
Subtitle of host publicationProceedings of the University Kebangsaan Malaysia, Faculty of Science and Technology 2017 Postgraduate Colloquium
PublisherAmerican Institute of Physics Inc.
Volume1940
ISBN (Electronic)9780735416321
DOIs
Publication statusPublished - 4 Apr 2018
Event2017 UKM FST Postgraduate Colloquium - Selangor, Malaysia
Duration: 12 Jul 201713 Jul 2017

Other

Other2017 UKM FST Postgraduate Colloquium
CountryMalaysia
CitySelangor
Period12/7/1713/7/17

Fingerprint

fungi
bacteria
isolation
screening
staphylococcus
pseudomonas
metabolites
homology
organisms
epidermis
agriculture
Bacillus
nutrients
medicine
industries

ASJC Scopus subject areas

  • Physics and Astronomy(all)

Cite this

Fikri, A. S. I., Abdul Rahman, I., Md Nor, N. S., & Hamzah, A. (2018). Isolation and identification of local bacteria endophyte and screening of its antimicrobial property against pathogenic bacteria and fungi. In 2017 UKM FST Postgraduate Colloquium: Proceedings of the University Kebangsaan Malaysia, Faculty of Science and Technology 2017 Postgraduate Colloquium (Vol. 1940). [020072] American Institute of Physics Inc.. https://doi.org/10.1063/1.5027987

Isolation and identification of local bacteria endophyte and screening of its antimicrobial property against pathogenic bacteria and fungi. / Fikri, Ahmad Syairazie Ibrahim; Abdul Rahman, Irman; Md Nor, Norefrina Shafinaz; Hamzah, Ainon.

2017 UKM FST Postgraduate Colloquium: Proceedings of the University Kebangsaan Malaysia, Faculty of Science and Technology 2017 Postgraduate Colloquium. Vol. 1940 American Institute of Physics Inc., 2018. 020072.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Fikri, ASI, Abdul Rahman, I, Md Nor, NS & Hamzah, A 2018, Isolation and identification of local bacteria endophyte and screening of its antimicrobial property against pathogenic bacteria and fungi. in 2017 UKM FST Postgraduate Colloquium: Proceedings of the University Kebangsaan Malaysia, Faculty of Science and Technology 2017 Postgraduate Colloquium. vol. 1940, 020072, American Institute of Physics Inc., 2017 UKM FST Postgraduate Colloquium, Selangor, Malaysia, 12/7/17. https://doi.org/10.1063/1.5027987
Fikri ASI, Abdul Rahman I, Md Nor NS, Hamzah A. Isolation and identification of local bacteria endophyte and screening of its antimicrobial property against pathogenic bacteria and fungi. In 2017 UKM FST Postgraduate Colloquium: Proceedings of the University Kebangsaan Malaysia, Faculty of Science and Technology 2017 Postgraduate Colloquium. Vol. 1940. American Institute of Physics Inc. 2018. 020072 https://doi.org/10.1063/1.5027987
Fikri, Ahmad Syairazie Ibrahim ; Abdul Rahman, Irman ; Md Nor, Norefrina Shafinaz ; Hamzah, Ainon. / Isolation and identification of local bacteria endophyte and screening of its antimicrobial property against pathogenic bacteria and fungi. 2017 UKM FST Postgraduate Colloquium: Proceedings of the University Kebangsaan Malaysia, Faculty of Science and Technology 2017 Postgraduate Colloquium. Vol. 1940 American Institute of Physics Inc., 2018.
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