Isolation and identification of lactic acid bacteria from local pickled mango with antibacterial potential

Nur Ilida Mohamad, Musaalbakri Abdul Manan, Norrakiah Abdullah Sani

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

A study was carried out to isolate and identify the lactic acid bacteria (LAB) from local pickled mango. The isolation of LAB was done using standard method and the isolated colonies with positive preliminary identification based on their morphology on agar, Gram-positive staining characteristics and their negative catalase reaction were chosen for identification with API 50 CH kit, the identification system based on phenotypical characteristics for the taxonomic identification of the genus Lactobacillus and related genera. A total of five LAB have been successfully isolated and identified as Lactobacillus fermentum (1 isolate), L. pentosus (2 isolates) and L. paracasei (2 isolates). The antibacterial activity of the identified LAB was conducted against ten commonly known foodborne bacterial pathogens using LAB cell cultures by agar spot test method. The antibacterial activity was also evaluated using the cell free supernatant (CFS) of the LAB through agar well diffusion method. All five LAB isolates were able to produce antibacterial activity that inhibited the tested pathogens.

Original languageEnglish
Title of host publication2018 UKM FST Postgraduate Colloquium
Subtitle of host publicationProceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2018 Postgraduate Colloquium
EditorsNoor Hayati Ahmad Rasol, Kamarulzaman Ibrahim, Siti Aishah Hasbullah, Mohammad Hafizuddin Hj. Jumali, Nazlina Ibrahim, Marlia Mohd Hanafiah, Mohd Talib Latif
PublisherAmerican Institute of Physics Inc.
ISBN (Electronic)9780735418431
DOIs
Publication statusPublished - 27 Jun 2019
Event2018 UKM FST Postgraduate Colloquium - Selangor, Malaysia
Duration: 4 Apr 20186 Apr 2018

Publication series

NameAIP Conference Proceedings
Volume2111
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Conference

Conference2018 UKM FST Postgraduate Colloquium
CountryMalaysia
CitySelangor
Period4/4/186/4/18

Fingerprint

lactic acid
mangoes
bacteria
lactic acid bacteria
isolation
bacterium
acid
agar
pathogens
pathogen
Lactobacillus pentosus
Lactobacillus paracasei
Lactobacillus fermentum
catalase
application programming interface
kits
system identification
staining
Lactobacillus
cell culture

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Ecology
  • Plant Science
  • Physics and Astronomy(all)
  • Nature and Landscape Conservation

Cite this

Mohamad, N. I., Manan, M. A., & Abdullah Sani, N. (2019). Isolation and identification of lactic acid bacteria from local pickled mango with antibacterial potential. In N. H. A. Rasol, K. Ibrahim, S. A. Hasbullah, M. H. H. Jumali, N. Ibrahim, M. M. Hanafiah, & M. T. Latif (Eds.), 2018 UKM FST Postgraduate Colloquium: Proceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2018 Postgraduate Colloquium [050011] (AIP Conference Proceedings; Vol. 2111). American Institute of Physics Inc.. https://doi.org/10.1063/1.5111259

Isolation and identification of lactic acid bacteria from local pickled mango with antibacterial potential. / Mohamad, Nur Ilida; Manan, Musaalbakri Abdul; Abdullah Sani, Norrakiah.

2018 UKM FST Postgraduate Colloquium: Proceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2018 Postgraduate Colloquium. ed. / Noor Hayati Ahmad Rasol; Kamarulzaman Ibrahim; Siti Aishah Hasbullah; Mohammad Hafizuddin Hj. Jumali; Nazlina Ibrahim; Marlia Mohd Hanafiah; Mohd Talib Latif. American Institute of Physics Inc., 2019. 050011 (AIP Conference Proceedings; Vol. 2111).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Mohamad, NI, Manan, MA & Abdullah Sani, N 2019, Isolation and identification of lactic acid bacteria from local pickled mango with antibacterial potential. in NHA Rasol, K Ibrahim, SA Hasbullah, MHH Jumali, N Ibrahim, MM Hanafiah & MT Latif (eds), 2018 UKM FST Postgraduate Colloquium: Proceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2018 Postgraduate Colloquium., 050011, AIP Conference Proceedings, vol. 2111, American Institute of Physics Inc., 2018 UKM FST Postgraduate Colloquium, Selangor, Malaysia, 4/4/18. https://doi.org/10.1063/1.5111259
Mohamad NI, Manan MA, Abdullah Sani N. Isolation and identification of lactic acid bacteria from local pickled mango with antibacterial potential. In Rasol NHA, Ibrahim K, Hasbullah SA, Jumali MHH, Ibrahim N, Hanafiah MM, Latif MT, editors, 2018 UKM FST Postgraduate Colloquium: Proceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2018 Postgraduate Colloquium. American Institute of Physics Inc. 2019. 050011. (AIP Conference Proceedings). https://doi.org/10.1063/1.5111259
Mohamad, Nur Ilida ; Manan, Musaalbakri Abdul ; Abdullah Sani, Norrakiah. / Isolation and identification of lactic acid bacteria from local pickled mango with antibacterial potential. 2018 UKM FST Postgraduate Colloquium: Proceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2018 Postgraduate Colloquium. editor / Noor Hayati Ahmad Rasol ; Kamarulzaman Ibrahim ; Siti Aishah Hasbullah ; Mohammad Hafizuddin Hj. Jumali ; Nazlina Ibrahim ; Marlia Mohd Hanafiah ; Mohd Talib Latif. American Institute of Physics Inc., 2019. (AIP Conference Proceedings).
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