Isolation and identification of Elizabethkingia meningoseptica from diseased African catfish Clarias gariepinus

A. A. Laith, Mazlan Abd. Ghaffar, M. A. Ambak, A. Jabar, M. Najiah

Research output: Contribution to journalArticle

Abstract

Two isolates of Elizabethkingia meningoseptica were successfully isolated from kidney and skin tissue of diseased African Catfish (Clarias gariepinus) in Malaysia. The percentage similarity of both physical and biochemical characteristics for the isolates from kidney (K1) and skin samples (S1) as determined by BBL-Crystal and API 20E were 99 % and 95.4%, respectively. Furthermore, both isolates were identified via 16S rRNA gene sequences and showed more than 97% homology to sequences deposited in GenBank. The API ZYM results were analogous for both strains, with only minor quantitative variations. However, the isolates from kidney sample (K1) showed higher levels of enzymatic activity reaction towards esterase lipase and leucine arylamidase. Moreover, the enzymatic activity of α-galactosidase was detected at low level in kidney isolate and absence in skin isolate.

Original languageEnglish
Pages (from-to)1070-1076
Number of pages7
JournalJournal of Microbiology, Biotechnology and Food Sciences
Volume6
Issue number4
DOIs
Publication statusPublished - 2017
Externally publishedYes

Fingerprint

Catfishes
Clarias gariepinus
catfish
kidneys
skin (animal)
Kidney
Galactosidases
galactosidases
Skin
Malaysia
Nucleic Acid Databases
Esterases
Sequence Homology
Lipase
rRNA Genes
Skin Diseases
Leucine
esterases
leucine
crystals

Keywords

  • BBL-Crystal
  • Clarias gariepinus
  • Elizabethkingia meningoseptica
  • Enzymatic activity
  • Polymerase chain reaction

ASJC Scopus subject areas

  • Food Science
  • Biotechnology
  • Molecular Biology
  • Microbiology

Cite this

Isolation and identification of Elizabethkingia meningoseptica from diseased African catfish Clarias gariepinus. / Laith, A. A.; Abd. Ghaffar, Mazlan; Ambak, M. A.; Jabar, A.; Najiah, M.

In: Journal of Microbiology, Biotechnology and Food Sciences, Vol. 6, No. 4, 2017, p. 1070-1076.

Research output: Contribution to journalArticle

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