Isolation and identification of catechol degrading bacteria

Research output: Contribution to journalArticle

Abstract

This research involves the isolation, identification, determination of bacterial growth, degradation rate and enzyme activity of catechol dioxygenase in bacteria. Eight bacteria were isolated from 3 locations: the oxidation pool, refinery pool and soil at the PETRONAS Oil Refinery Plant in Kerteh, Terengganu, Malaysia. Out of the eight isolated bacteria, seven were Gram negative and one Gram positive, bacilli. Two bacteria were chosen based on correlation from the screening results of spread plate method and optical density method. Both bacteria were identified as Pseudomonas pickettii (88.3%) and Agrobacterium rqdiobacter (99.8%) via the API Kit 20NE. Based on the growth profile of bacteria, the growth rate of A.radiobacter was higher than P. pickettii, with 0.045 h -1 compared to 0.011 h-1. A.radiobacter showed higher degradation rate than P.pickettii, with 1.037 mgL-1 h -1 for A.radiobacter and 0.910 mgl-1h-1 for P.pickettii. Enzyme assay of catechol 1,2-dioxygenase and catechol 2,3-dioxygenase was conducted on both bacteria and the result was a higher enzyme activity in A.radiobacter than P.pickettii. Enzyme activity and specific activity of catechol 1,2-dioxygenase in A.radiobacter was 0.085 μmol min -1 mL-1 and 0.167 μmol min-1 mg-1 respectively while P. pickettii was 0.042μmol min-1 mL -1 and 0.115 μmol min-1 mg-1. Enzyme activity and specific activity of catechol 2,3-dioxygenase in A.radiobacter was 0.002 μmol min-1 mL-1 and 0.004 μmol min -1 mg-1 respectively while P. pickettii was 0.001μmol min-1 mL-1 and 0.003μmol min-1 mg -1. The overall results of this study showed that A.radiobacter is a better candidate of the two bacteria in degrading catechol.

Original languageEnglish
Pages (from-to)881-890
Number of pages10
JournalJournal of Pure and Applied Microbiology
Volume7
Issue number2
Publication statusPublished - Jun 2013

Fingerprint

Bacteria
Catechol 1,2-Dioxygenase
Catechol 2,3-Dioxygenase
Enzymes
Growth
Dioxygenases
Agrobacterium
Plant Oils
catechol
Malaysia
Enzyme Assays
Pseudomonas
Bacillus
Soil
Research

Keywords

  • Agrobacterium
  • Catechol
  • Catechol dioxygenase
  • Pseudomonas

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Biotechnology
  • Microbiology

Cite this

Isolation and identification of catechol degrading bacteria. / K. Nadarajah, Kalaivani; Tian, Tan Shi.

In: Journal of Pure and Applied Microbiology, Vol. 7, No. 2, 06.2013, p. 881-890.

Research output: Contribution to journalArticle

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abstract = "This research involves the isolation, identification, determination of bacterial growth, degradation rate and enzyme activity of catechol dioxygenase in bacteria. Eight bacteria were isolated from 3 locations: the oxidation pool, refinery pool and soil at the PETRONAS Oil Refinery Plant in Kerteh, Terengganu, Malaysia. Out of the eight isolated bacteria, seven were Gram negative and one Gram positive, bacilli. Two bacteria were chosen based on correlation from the screening results of spread plate method and optical density method. Both bacteria were identified as Pseudomonas pickettii (88.3{\%}) and Agrobacterium rqdiobacter (99.8{\%}) via the API Kit 20NE. Based on the growth profile of bacteria, the growth rate of A.radiobacter was higher than P. pickettii, with 0.045 h -1 compared to 0.011 h-1. A.radiobacter showed higher degradation rate than P.pickettii, with 1.037 mgL-1 h -1 for A.radiobacter and 0.910 mgl-1h-1 for P.pickettii. Enzyme assay of catechol 1,2-dioxygenase and catechol 2,3-dioxygenase was conducted on both bacteria and the result was a higher enzyme activity in A.radiobacter than P.pickettii. Enzyme activity and specific activity of catechol 1,2-dioxygenase in A.radiobacter was 0.085 μmol min -1 mL-1 and 0.167 μmol min-1 mg-1 respectively while P. pickettii was 0.042μmol min-1 mL -1 and 0.115 μmol min-1 mg-1. Enzyme activity and specific activity of catechol 2,3-dioxygenase in A.radiobacter was 0.002 μmol min-1 mL-1 and 0.004 μmol min -1 mg-1 respectively while P. pickettii was 0.001μmol min-1 mL-1 and 0.003μmol min-1 mg -1. The overall results of this study showed that A.radiobacter is a better candidate of the two bacteria in degrading catechol.",
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