Isolation and Identification of an Anaerobic Dissimilatory Fe(III)-Reducing Bacterium, Shewanella putrefaciens IR-1

Moon Sik Hyun, Byung Hong Kim, In Seop Chang, Hyung Soo Park, Hyung Joo Kim, Gwang Tae Kim, Mi A. Kim, Doo Hyun Park

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

In order to isolate a Fe(III)-reducer from the natural environment, soil samples were collected from various paddy fields and enriched with ferric citrate as a source of Fe(III) under anaerobic condition. Since the enrichment culture was serially performed, the Fe(UI)-reduction activity increased. The final enriched culture that showed the highest Fe(III)-reduction activity was serially diluted and cultivated on an agar plate containing lactate and ferric citrate in an anaerobic glove box. A Gram negative, motile, rod-shaped and facultative anaerobic Fe(III)-reducer was isolated based on its highest Fe(III)-reduction activity. Bacterial growth was coupled with oxidation of lactate to Fe(III)-reduction, but the isolate fermented pyruvate without Fe(III). The isolate reduced an insoluble ferric iron (FeOOH) as well as a soluble ferric iron (ferric citrate). Using the BBL crystal enteric/non-fermentor identification kit and 16S rDNA sequence analysis, the isolate was identified as Shewanella putrefaciens IR-1.

Original languageEnglish
Pages (from-to)206-212
Number of pages7
JournalJournal of Microbiology
Volume37
Issue number4
Publication statusPublished - 1999
Externally publishedYes

Fingerprint

Shewanella putrefaciens
Bacteria
Lactic Acid
Iron
Ribosomal DNA
Pyruvic Acid
Agar
Sequence Analysis
Soil
Growth
ferric citrate

Keywords

  • Fe(III)-reducer
  • Metal reduction
  • Shewanella putrefaciens

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Microbiology

Cite this

Hyun, M. S., Kim, B. H., Chang, I. S., Park, H. S., Kim, H. J., Kim, G. T., ... Park, D. H. (1999). Isolation and Identification of an Anaerobic Dissimilatory Fe(III)-Reducing Bacterium, Shewanella putrefaciens IR-1. Journal of Microbiology, 37(4), 206-212.

Isolation and Identification of an Anaerobic Dissimilatory Fe(III)-Reducing Bacterium, Shewanella putrefaciens IR-1. / Hyun, Moon Sik; Kim, Byung Hong; Chang, In Seop; Park, Hyung Soo; Kim, Hyung Joo; Kim, Gwang Tae; Kim, Mi A.; Park, Doo Hyun.

In: Journal of Microbiology, Vol. 37, No. 4, 1999, p. 206-212.

Research output: Contribution to journalArticle

Hyun, MS, Kim, BH, Chang, IS, Park, HS, Kim, HJ, Kim, GT, Kim, MA & Park, DH 1999, 'Isolation and Identification of an Anaerobic Dissimilatory Fe(III)-Reducing Bacterium, Shewanella putrefaciens IR-1', Journal of Microbiology, vol. 37, no. 4, pp. 206-212.
Hyun, Moon Sik ; Kim, Byung Hong ; Chang, In Seop ; Park, Hyung Soo ; Kim, Hyung Joo ; Kim, Gwang Tae ; Kim, Mi A. ; Park, Doo Hyun. / Isolation and Identification of an Anaerobic Dissimilatory Fe(III)-Reducing Bacterium, Shewanella putrefaciens IR-1. In: Journal of Microbiology. 1999 ; Vol. 37, No. 4. pp. 206-212.
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