Isolation and characterization of oil palm constitutive promoter derived from ubiquitin extension protein (uep1) gene

Subhi Siti Masura, Ghulam Kadir Ahmad Parveez, Ismanizan Ismail

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The ubiquitin extension protein (uep1) gene was identified as a constitutively expressed gene in oil palm. We have isolated and characterized the 5' region of the oil palm uep1 gene, which contains an 828. bp sequence upstream of the uep1 translational start site. Construction of a pUEP1 transformation vector, which contains gusA reporter gene under the control of uep1 promoter, was carried out for functional analysis of the promoter through transient expression studies. It was found that the 5' region of uep1 functions as a constitutive promoter in oil palm and could drive GUS expression in all tissues tested, including embryogenic calli, embryoid, immature embryo, young leaflet from mature palm, green leaf, mesocarp and meristematic tissues (shoot tip). This promoter could also be used in dicot systems as it was demonstrated to be capable of driving gusA gene expression in tobacco.

Original languageEnglish
Pages (from-to)289-299
Number of pages11
JournalNew Biotechnology
Volume27
Issue number4
DOIs
Publication statusPublished - Sep 2010

Fingerprint

Palm oil
Ubiquitin
Oils
Genes
Proteins
Tissue
Functional analysis
Tobacco
Bony Callus
Reporter Genes
Gene expression
Embryonic Structures
Gene Expression

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology
  • Bioengineering
  • Medicine(all)

Cite this

Isolation and characterization of oil palm constitutive promoter derived from ubiquitin extension protein (uep1) gene. / Masura, Subhi Siti; Parveez, Ghulam Kadir Ahmad; Ismail, Ismanizan.

In: New Biotechnology, Vol. 27, No. 4, 09.2010, p. 289-299.

Research output: Contribution to journalArticle

@article{9b4331d68d5b4fd5be92b26f5915bd0a,
title = "Isolation and characterization of oil palm constitutive promoter derived from ubiquitin extension protein (uep1) gene",
abstract = "The ubiquitin extension protein (uep1) gene was identified as a constitutively expressed gene in oil palm. We have isolated and characterized the 5' region of the oil palm uep1 gene, which contains an 828. bp sequence upstream of the uep1 translational start site. Construction of a pUEP1 transformation vector, which contains gusA reporter gene under the control of uep1 promoter, was carried out for functional analysis of the promoter through transient expression studies. It was found that the 5' region of uep1 functions as a constitutive promoter in oil palm and could drive GUS expression in all tissues tested, including embryogenic calli, embryoid, immature embryo, young leaflet from mature palm, green leaf, mesocarp and meristematic tissues (shoot tip). This promoter could also be used in dicot systems as it was demonstrated to be capable of driving gusA gene expression in tobacco.",
author = "Masura, {Subhi Siti} and Parveez, {Ghulam Kadir Ahmad} and Ismanizan Ismail",
year = "2010",
month = "9",
doi = "10.1016/j.nbt.2010.01.337",
language = "English",
volume = "27",
pages = "289--299",
journal = "New Biotechnology",
issn = "1871-6784",
publisher = "Elsevier",
number = "4",

}

TY - JOUR

T1 - Isolation and characterization of oil palm constitutive promoter derived from ubiquitin extension protein (uep1) gene

AU - Masura, Subhi Siti

AU - Parveez, Ghulam Kadir Ahmad

AU - Ismail, Ismanizan

PY - 2010/9

Y1 - 2010/9

N2 - The ubiquitin extension protein (uep1) gene was identified as a constitutively expressed gene in oil palm. We have isolated and characterized the 5' region of the oil palm uep1 gene, which contains an 828. bp sequence upstream of the uep1 translational start site. Construction of a pUEP1 transformation vector, which contains gusA reporter gene under the control of uep1 promoter, was carried out for functional analysis of the promoter through transient expression studies. It was found that the 5' region of uep1 functions as a constitutive promoter in oil palm and could drive GUS expression in all tissues tested, including embryogenic calli, embryoid, immature embryo, young leaflet from mature palm, green leaf, mesocarp and meristematic tissues (shoot tip). This promoter could also be used in dicot systems as it was demonstrated to be capable of driving gusA gene expression in tobacco.

AB - The ubiquitin extension protein (uep1) gene was identified as a constitutively expressed gene in oil palm. We have isolated and characterized the 5' region of the oil palm uep1 gene, which contains an 828. bp sequence upstream of the uep1 translational start site. Construction of a pUEP1 transformation vector, which contains gusA reporter gene under the control of uep1 promoter, was carried out for functional analysis of the promoter through transient expression studies. It was found that the 5' region of uep1 functions as a constitutive promoter in oil palm and could drive GUS expression in all tissues tested, including embryogenic calli, embryoid, immature embryo, young leaflet from mature palm, green leaf, mesocarp and meristematic tissues (shoot tip). This promoter could also be used in dicot systems as it was demonstrated to be capable of driving gusA gene expression in tobacco.

UR - http://www.scopus.com/inward/record.url?scp=77955093729&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77955093729&partnerID=8YFLogxK

U2 - 10.1016/j.nbt.2010.01.337

DO - 10.1016/j.nbt.2010.01.337

M3 - Article

C2 - 20123048

AN - SCOPUS:77955093729

VL - 27

SP - 289

EP - 299

JO - New Biotechnology

JF - New Biotechnology

SN - 1871-6784

IS - 4

ER -