Abstract
Chitosan from Cunninghamella sp. A1, Absidia butleri 2A1, Rhizopus sp UKM21 and Mucor sp UKM13 was isolated and characterized in this study. The effect of medium and nitrogen sources on the yield of chitosan was investigated. The amount of chitosan produced by these isolates was higher than previously reported, with Cunninghamella sp. A1, Absidia butleri 2A1, Rhizopus sp UKM21 and Mucor sp UKM13 producing 2.59 ± 0.5, 2.20 ± 0.25, 2.05 ± 0.30 and 1.96 ± 0.4 g/L chitosan respectively. The highest yield was obtained in Yeast-Peptone-Glucose (YPG) medium supplemented with peptone (range 0.82-2.92 g/L). In addition to affecting yield, these parameters influenced the degree of deacetylation (DD) and molecular weight (MW) of the fungal chitosan. The DD and MW for the fungal chitosan isolated in this study was between 80-95% and 5.1-35.9 × 104 Dalton respectively. Since the chitosan isolated had high DD and LMW, we tested its ability as an antimicrobial agent. The disc diffusion and Minimum Inhibitory Concentration (MIC) studies indicate that the fungal chitosan had higher inhibitory effects on Gram positive bacteria compared to the Gram negative organisms tested in this study. The fungal chitosan also showed bacteriocidal effects on Candida spp.
| Original language | English |
|---|---|
| Pages (from-to) | 403-410 |
| Number of pages | 8 |
| Journal | Journal of Pure and Applied Microbiology |
| Volume | 3 |
| Issue number | 2 |
| Publication status | Published - Oct 2009 |
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Keywords
- Antimicrobial
- Bacteriocidal
- Chitosan
- Degree deacetylation (DD)
- Fungal
- Molecular weight (MW)
ASJC Scopus subject areas
- Applied Microbiology and Biotechnology
- Biotechnology
- Microbiology
Cite this
Isolation and characterization of fungal chitosan from Malaysian isolates. / K. Nadarajah, Kalaivani; Cheng, T. Y W.
In: Journal of Pure and Applied Microbiology, Vol. 3, No. 2, 10.2009, p. 403-410.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Isolation and characterization of fungal chitosan from Malaysian isolates
AU - K. Nadarajah, Kalaivani
AU - Cheng, T. Y W
PY - 2009/10
Y1 - 2009/10
N2 - Chitosan from Cunninghamella sp. A1, Absidia butleri 2A1, Rhizopus sp UKM21 and Mucor sp UKM13 was isolated and characterized in this study. The effect of medium and nitrogen sources on the yield of chitosan was investigated. The amount of chitosan produced by these isolates was higher than previously reported, with Cunninghamella sp. A1, Absidia butleri 2A1, Rhizopus sp UKM21 and Mucor sp UKM13 producing 2.59 ± 0.5, 2.20 ± 0.25, 2.05 ± 0.30 and 1.96 ± 0.4 g/L chitosan respectively. The highest yield was obtained in Yeast-Peptone-Glucose (YPG) medium supplemented with peptone (range 0.82-2.92 g/L). In addition to affecting yield, these parameters influenced the degree of deacetylation (DD) and molecular weight (MW) of the fungal chitosan. The DD and MW for the fungal chitosan isolated in this study was between 80-95% and 5.1-35.9 × 104 Dalton respectively. Since the chitosan isolated had high DD and LMW, we tested its ability as an antimicrobial agent. The disc diffusion and Minimum Inhibitory Concentration (MIC) studies indicate that the fungal chitosan had higher inhibitory effects on Gram positive bacteria compared to the Gram negative organisms tested in this study. The fungal chitosan also showed bacteriocidal effects on Candida spp.
AB - Chitosan from Cunninghamella sp. A1, Absidia butleri 2A1, Rhizopus sp UKM21 and Mucor sp UKM13 was isolated and characterized in this study. The effect of medium and nitrogen sources on the yield of chitosan was investigated. The amount of chitosan produced by these isolates was higher than previously reported, with Cunninghamella sp. A1, Absidia butleri 2A1, Rhizopus sp UKM21 and Mucor sp UKM13 producing 2.59 ± 0.5, 2.20 ± 0.25, 2.05 ± 0.30 and 1.96 ± 0.4 g/L chitosan respectively. The highest yield was obtained in Yeast-Peptone-Glucose (YPG) medium supplemented with peptone (range 0.82-2.92 g/L). In addition to affecting yield, these parameters influenced the degree of deacetylation (DD) and molecular weight (MW) of the fungal chitosan. The DD and MW for the fungal chitosan isolated in this study was between 80-95% and 5.1-35.9 × 104 Dalton respectively. Since the chitosan isolated had high DD and LMW, we tested its ability as an antimicrobial agent. The disc diffusion and Minimum Inhibitory Concentration (MIC) studies indicate that the fungal chitosan had higher inhibitory effects on Gram positive bacteria compared to the Gram negative organisms tested in this study. The fungal chitosan also showed bacteriocidal effects on Candida spp.
KW - Antimicrobial
KW - Bacteriocidal
KW - Chitosan
KW - Degree deacetylation (DD)
KW - Fungal
KW - Molecular weight (MW)
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UR - http://www.scopus.com/inward/citedby.url?scp=83455210573&partnerID=8YFLogxK
M3 - Article
VL - 3
SP - 403
EP - 410
JO - Journal of Pure and Applied Microbiology
JF - Journal of Pure and Applied Microbiology
SN - 0973-7510
IS - 2
ER -