Isolation and characterization of full-length putative Alcohol dehydrogenase genes from Polygonum minus

Nur Athirah Abd Hamid, Ismanizan Ismail

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Polygonum minus, locally named as Kesum is an aromatic herb which is high in secondary metabolite content. Alcohol dehydrogenase is an important enzyme that catalyzes the reversible oxidation of alcohol and aldehyde with the presence of NAD(P)(H) as co-factor. The main focus of this research is to identify the gene of ADH. The total RNA was extracted from leaves of P. minus which was treated with 150 μM Jasmonic acid. Full-length cDNA sequence of ADH was isolated via rapid amplification cDNA end (RACE). Subsequently, in silico analysis was conducted on the fulllength cDNA sequence and PCR was done on genomic DNA to determine the exon and intron organization. Two sequences of ADH, designated as PmADH1 and PmADH2 were successfully isolated. Both sequences have ORF of 801 bp which encode 266 aa residues. Nucleotide sequence comparison of PmADH1 and PmADH2 indicated that both sequences are highly similar at the ORF region but divergent in the 3 untranslated regions (UTR). The amino acid is differ at the 107 residue; PmADH1 contains Gly (G) residue while PmADH2 contains Cys (C) residue. The intron-exon organization pattern of both sequences are also same, with 3 introns and 4 exons. Based on in silico analysis, both sequences contain "classical" short chain alcohol dehydrogenases/ reductases ((c) SDRs) conserved domain. The results suggest that both sequences are the members of short chain alcohol dehydrogenase family.

Original languageEnglish
Title of host publicationAIP Conference Proceedings
Pages234-237
Number of pages4
Volume1571
DOIs
Publication statusPublished - 2013
Event2013 UKM Faculty of Science and Technology Post-Graduate Colloquium - Selangor
Duration: 3 Jul 20134 Jul 2013

Other

Other2013 UKM Faculty of Science and Technology Post-Graduate Colloquium
CitySelangor
Period3/7/134/7/13

Fingerprint

dehydrogenases
genes
isolation
alcohols
metabolites
nucleotides
aldehydes
leaves
amino acids
enzymes
deoxyribonucleic acid
oxidation
acids

Keywords

  • DNA
  • Electrophoresis
  • Genomics
  • RNA
  • Sequence analysis

ASJC Scopus subject areas

  • Physics and Astronomy(all)

Cite this

Isolation and characterization of full-length putative Alcohol dehydrogenase genes from Polygonum minus. / Hamid, Nur Athirah Abd; Ismail, Ismanizan.

AIP Conference Proceedings. Vol. 1571 2013. p. 234-237.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Hamid, NAA & Ismail, I 2013, Isolation and characterization of full-length putative Alcohol dehydrogenase genes from Polygonum minus. in AIP Conference Proceedings. vol. 1571, pp. 234-237, 2013 UKM Faculty of Science and Technology Post-Graduate Colloquium, Selangor, 3/7/13. https://doi.org/10.1063/1.4858660
Hamid, Nur Athirah Abd ; Ismail, Ismanizan. / Isolation and characterization of full-length putative Alcohol dehydrogenase genes from Polygonum minus. AIP Conference Proceedings. Vol. 1571 2013. pp. 234-237
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