Isolation and characterization of encoded formaldehyde responsive gene from the plant Dieffenbachia compacta

Research output: Contribution to journalArticle

Abstract

Plants have several defence mechanisms to survive in a stressful environment. Formaldehyde is one of the indoor air pollutants that can cause cancer. Ornamental plant Dieffenbachia compacta can be used as biological control for pollutants in the indoor air environment. This study was conducted to isolate the genes that response for detoxifying formaldehyde in Dieffenbachia compacta. Methods used to isolate the gene is by using reverse transcription PCR (RT-PCR). A total of 735 base pairs was isolated using a primer from Epipr emnum aur eum FALDH genes. Nucleotide sequence analysis showed that the sequence similarities of 85% and 84% to the FALDH mRNA for Epipremnum aureum and Populus trichocarpa respectively. While for amino acid 92% similarities were found against Epipr emnum aur eum and Populus trichocar pa. A total of 87 amino acids that were encoded from 261 base pairs using the Open Reading Frame Finder. Our finding showed that the isolated Dieffenbachia compacta gene is the gene for glutathione-dependent formaldehyde dehydrogenase (FALDH). As a conclusion, formaldehyde metabolism in plants is regulated by the enzyme glutathione-dependent formaldehyde dehydrogenase (FALDH). The activity of FALDH indicates that this enzyme might be part of the universal metabolism pathway shared by a variety of organisms.

Original languageEnglish
Pages (from-to)435-438
Number of pages4
JournalNature Environment and Pollution Technology
Volume14
Issue number2
Publication statusPublished - 1 Jun 2015

Fingerprint

formaldehyde
Formaldehyde
Genes
gene
indoor air
Metabolism
Amino acids
Enzymes
amino acid
metabolism
enzyme
defense mechanism
Transcription
Nucleotides
Air
biological control
cancer
Oxidoreductases
pollutant

Keywords

  • (FALDH)
  • Dieffenbachia compacta
  • Formaldehyde dehydrogenase
  • Formaldehyde responsive
  • Gene
  • Glutathione-dependent

ASJC Scopus subject areas

  • Renewable Energy, Sustainability and the Environment
  • Environmental Science(all)

Cite this

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title = "Isolation and characterization of encoded formaldehyde responsive gene from the plant Dieffenbachia compacta",
abstract = "Plants have several defence mechanisms to survive in a stressful environment. Formaldehyde is one of the indoor air pollutants that can cause cancer. Ornamental plant Dieffenbachia compacta can be used as biological control for pollutants in the indoor air environment. This study was conducted to isolate the genes that response for detoxifying formaldehyde in Dieffenbachia compacta. Methods used to isolate the gene is by using reverse transcription PCR (RT-PCR). A total of 735 base pairs was isolated using a primer from Epipr emnum aur eum FALDH genes. Nucleotide sequence analysis showed that the sequence similarities of 85{\%} and 84{\%} to the FALDH mRNA for Epipremnum aureum and Populus trichocarpa respectively. While for amino acid 92{\%} similarities were found against Epipr emnum aur eum and Populus trichocar pa. A total of 87 amino acids that were encoded from 261 base pairs using the Open Reading Frame Finder. Our finding showed that the isolated Dieffenbachia compacta gene is the gene for glutathione-dependent formaldehyde dehydrogenase (FALDH). As a conclusion, formaldehyde metabolism in plants is regulated by the enzyme glutathione-dependent formaldehyde dehydrogenase (FALDH). The activity of FALDH indicates that this enzyme might be part of the universal metabolism pathway shared by a variety of organisms.",
keywords = "(FALDH), Dieffenbachia compacta, Formaldehyde dehydrogenase, Formaldehyde responsive, Gene, Glutathione-dependent",
author = "Anuar Ithnin and Ibrahim, {Mira Azilah} and {Che Mohd. Zain}, {Che Radziah} and {Abdul Halim}, Azhar",
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AU - Ithnin, Anuar

AU - Ibrahim, Mira Azilah

AU - Che Mohd. Zain, Che Radziah

AU - Abdul Halim, Azhar

PY - 2015/6/1

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N2 - Plants have several defence mechanisms to survive in a stressful environment. Formaldehyde is one of the indoor air pollutants that can cause cancer. Ornamental plant Dieffenbachia compacta can be used as biological control for pollutants in the indoor air environment. This study was conducted to isolate the genes that response for detoxifying formaldehyde in Dieffenbachia compacta. Methods used to isolate the gene is by using reverse transcription PCR (RT-PCR). A total of 735 base pairs was isolated using a primer from Epipr emnum aur eum FALDH genes. Nucleotide sequence analysis showed that the sequence similarities of 85% and 84% to the FALDH mRNA for Epipremnum aureum and Populus trichocarpa respectively. While for amino acid 92% similarities were found against Epipr emnum aur eum and Populus trichocar pa. A total of 87 amino acids that were encoded from 261 base pairs using the Open Reading Frame Finder. Our finding showed that the isolated Dieffenbachia compacta gene is the gene for glutathione-dependent formaldehyde dehydrogenase (FALDH). As a conclusion, formaldehyde metabolism in plants is regulated by the enzyme glutathione-dependent formaldehyde dehydrogenase (FALDH). The activity of FALDH indicates that this enzyme might be part of the universal metabolism pathway shared by a variety of organisms.

AB - Plants have several defence mechanisms to survive in a stressful environment. Formaldehyde is one of the indoor air pollutants that can cause cancer. Ornamental plant Dieffenbachia compacta can be used as biological control for pollutants in the indoor air environment. This study was conducted to isolate the genes that response for detoxifying formaldehyde in Dieffenbachia compacta. Methods used to isolate the gene is by using reverse transcription PCR (RT-PCR). A total of 735 base pairs was isolated using a primer from Epipr emnum aur eum FALDH genes. Nucleotide sequence analysis showed that the sequence similarities of 85% and 84% to the FALDH mRNA for Epipremnum aureum and Populus trichocarpa respectively. While for amino acid 92% similarities were found against Epipr emnum aur eum and Populus trichocar pa. A total of 87 amino acids that were encoded from 261 base pairs using the Open Reading Frame Finder. Our finding showed that the isolated Dieffenbachia compacta gene is the gene for glutathione-dependent formaldehyde dehydrogenase (FALDH). As a conclusion, formaldehyde metabolism in plants is regulated by the enzyme glutathione-dependent formaldehyde dehydrogenase (FALDH). The activity of FALDH indicates that this enzyme might be part of the universal metabolism pathway shared by a variety of organisms.

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