Isolation and characterization of dental pulp stem cells from murine incisors

M. R. Nur Akmal, Z. A. Intan Zarina, Rohaya Megat Abdul Wahab, Sahidan Senafi, Z. A. Zaidah, Shahrul Hisham Zainal Ariffin

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Studies have proven that dental pulp contains cells with stem cell activity. Although mice are widely used as a model organism, murine dental pulp stem cells (mDPSCs) are still poorly characterized. In this study, we aim to provide a modified and cost-effective method for isolating mDPSCs and to characterize the isolated cells using molecular markers. Mice incisors' dental pulps were digested in collagenase 1A and the cell suspensions cultured in α-MEM supplemented with 20% (v/v) fetal bovine serum and 1% (v/v) penicillin-streptomycin. The morphology of the cells was observed and passage 4 cells were subjected to molecular characterization using RT-PCR. We found that the freshly isolated dental pulp cells through enzymatic dissociation method showed a heterogeneous cell morphology which became more homogeneous following cell passage. Semi-quantitative RT-PCR showed analysis that the murine incisors' DPSCs are Cdl05+, Cdl3+, Cd29+, Cdl46+, Cdl66+, Cd90Chlow, Cd73low, Cdl05- and Cd34-. Based on these findings, we concluded that dental pulp stem cells from murine incisor could be easily isolated through the method described and the incisor's dental pulp could be a source of mesenchymal stem cells. Further studies are required to explore the differentiation potential of the isolated mDPSC in vitro.

Original languageEnglish
Pages (from-to)327-331
Number of pages5
JournalJournal of Biological Sciences
Volume14
Issue number4
DOIs
Publication statusPublished - 2014

Fingerprint

Dental Pulp
Incisor
Stem Cells
Polymerase Chain Reaction
Collagenases
Streptomycin
Mesenchymal Stromal Cells
Penicillins
Cultured Cells
Suspensions
Costs and Cost Analysis

Keywords

  • Dental pulp
  • Murine incisor
  • Semi-quantitative RT-PCR
  • Stem cell

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Medicine

Cite this

Isolation and characterization of dental pulp stem cells from murine incisors. / Nur Akmal, M. R.; Intan Zarina, Z. A.; Megat Abdul Wahab, Rohaya; Senafi, Sahidan; Zaidah, Z. A.; Zainal Ariffin, Shahrul Hisham.

In: Journal of Biological Sciences, Vol. 14, No. 4, 2014, p. 327-331.

Research output: Contribution to journalArticle

Nur Akmal, M. R. ; Intan Zarina, Z. A. ; Megat Abdul Wahab, Rohaya ; Senafi, Sahidan ; Zaidah, Z. A. ; Zainal Ariffin, Shahrul Hisham. / Isolation and characterization of dental pulp stem cells from murine incisors. In: Journal of Biological Sciences. 2014 ; Vol. 14, No. 4. pp. 327-331.
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