Isolation and characterization of bacteria degrading Sumandak and South Angsi oils

Ainon Hamzah, Amir Rabu, Raja Farzarul Hanim Raja Azmy, Noor Ainni Yussoff

    Research output: Contribution to journalArticle

    36 Citations (Scopus)

    Abstract

    Four species of bacteria, Acinetobacter lwoffii, Aeromonas hydrophila, Pseudomonas aeruginosa and Pseudomonas pulida, were isolated from soil contaminated with hydrocarbons and selected for the determination of growth requirements and the ability to degrade petroleum hydrocarbon. The bacteria were grown in mineral salt medium (MSM) supplemented with two types of crude oil, either Sumandak or South Angsi at 1% (vlv) concentration. The optimum pH for growth of A. hydrophila and P. aeruginosa was 6.5 when grown with Sumandak and South Angsi oil. For A. lwoffii and P. putida the optimum pH for growth with Sumandak and South Angsi oil was 6.5 or 7.0, respectively. The growth of P. aeruginosa was the highest in MSM when supplemented with 1 % South Angsi oil and 0.5% tryptone at pH 6.5 while, in Sumandak oil the growth was the highest when yeast extract was added. Gas chromatography analysis showed that the South Angsi crude oil components of C12 to C25 were more extensively degraded by A. lwoffii after 24 h of incubation compared to the other bacteria over the same period.

    Original languageEnglish
    Pages (from-to)161-168
    Number of pages8
    JournalSains Malaysiana
    Volume39
    Issue number2
    Publication statusPublished - Apr 2010

    Fingerprint

    Acinetobacter lwoffii
    oils
    bacteria
    Pseudomonas aeruginosa
    Aeromonas hydrophila
    hydrocarbons
    minerals
    salts
    yeast extract
    polluted soils
    petroleum
    Pseudomonas
    gas chromatography

    Keywords

    • Bacteria
    • Biodegradation
    • Crude oil
    • Petroleum hydrocarbon

    ASJC Scopus subject areas

    • General

    Cite this

    Hamzah, A., Rabu, A., Azmy, R. F. H. R., & Yussoff, N. A. (2010). Isolation and characterization of bacteria degrading Sumandak and South Angsi oils. Sains Malaysiana, 39(2), 161-168.

    Isolation and characterization of bacteria degrading Sumandak and South Angsi oils. / Hamzah, Ainon; Rabu, Amir; Azmy, Raja Farzarul Hanim Raja; Yussoff, Noor Ainni.

    In: Sains Malaysiana, Vol. 39, No. 2, 04.2010, p. 161-168.

    Research output: Contribution to journalArticle

    Hamzah, A, Rabu, A, Azmy, RFHR & Yussoff, NA 2010, 'Isolation and characterization of bacteria degrading Sumandak and South Angsi oils', Sains Malaysiana, vol. 39, no. 2, pp. 161-168.
    Hamzah A, Rabu A, Azmy RFHR, Yussoff NA. Isolation and characterization of bacteria degrading Sumandak and South Angsi oils. Sains Malaysiana. 2010 Apr;39(2):161-168.
    Hamzah, Ainon ; Rabu, Amir ; Azmy, Raja Farzarul Hanim Raja ; Yussoff, Noor Ainni. / Isolation and characterization of bacteria degrading Sumandak and South Angsi oils. In: Sains Malaysiana. 2010 ; Vol. 39, No. 2. pp. 161-168.
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