Isolation and characterization of a novel endophytic Streptomyces SUK 06 with antimicrobial activity from Malaysian plant

Norazli Ghadin, Noraziah Mohamad Zin, Vikineswary Sabaratnam, Norhidayah Badya, Dayang Fredalina Basri, Hiang Lian Hing, Nik Marzuki Sidik

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

In search of new antimicrobial agents, samples of plant stem which were originally used as traditional medicine were collected from various parts of peninsular Malaysia. The isolate, namely SUK 06 from Thottea grandiflora (family-Aristolchieaceae) was cultured on International Streptomyces Project medium (ISP 2). Macroscopically, the bacteria surface appeared to be smooth with granule texture, irregular folding, dry and chalky and showed a substrate mycelium with grey in color. By scanning electron microscope, this isolate had a unique spiral filament structure. Molecular biological studies on the 16S rRNA revealed that it was distinct from all other genetic accessions of Streptomycetes in GenBank, but bear some genetic similarity to other Streptomycetes. The secondary metabolites extracted by ethyl acetate showed demonstrable killing activity against one or more pathogenic bacteria and inhibition zones (mm) were as followed: Bacilus subtilis (17 mm), Pseudomonas aeruginosa ATCC 27853 (20 mm), Bacillus cereus (22 mm), Pleisiomonas shigelloides (20 mm) and MRSA ATCC 700699 (37 mm). Nevertheless, there were some antifungal activity measured as a percentage of inhibition (%) against Fusarium solani (62%), Aspergillus fumigatus (44%), Pythium ultimum (20%), Phytophthora erythroseptica (23%) and Geothrichum candidum (23%).

Original languageEnglish
Pages (from-to)189-194
Number of pages6
JournalAsian Journal of Plant Sciences
Volume7
Issue number2
Publication statusPublished - 15 Feb 2008

Fingerprint

Streptomyces
Phytophthora erythroseptica
anti-infective agents
Pythium ultimum
Fusarium solani
Aspergillus fumigatus
bacteria
Bacillus cereus
traditional medicine
scanning electron microscopes
Pseudomonas aeruginosa
Malaysia
secondary metabolites
mycelium
granules
texture
ribosomal RNA
stems
color
sampling

Keywords

  • 16S rRNA
  • Antimicrobial
  • Endophytes
  • Fusarium solani
  • MRSA
  • Streptomycetes

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science

Cite this

Isolation and characterization of a novel endophytic Streptomyces SUK 06 with antimicrobial activity from Malaysian plant. / Ghadin, Norazli; Mohamad Zin, Noraziah; Sabaratnam, Vikineswary; Badya, Norhidayah; Basri, Dayang Fredalina; Hing, Hiang Lian; Sidik, Nik Marzuki.

In: Asian Journal of Plant Sciences, Vol. 7, No. 2, 15.02.2008, p. 189-194.

Research output: Contribution to journalArticle

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