Isolation and characterisation of phages targeting clinical Pseudomonas aeruginosa carrying virulence genes

Nur Afifah Binti Jamil, Abeer Ameen Baqer, Siti Noor Adnalizawati Adnan, Ainulkhir Hussin, Norefrina Shafinaz Md Nor

Research output: Contribution to journalArticle

Abstract

Aims: The aim of the study was to isolate and characterise bacteriophages specific to Pseudomonas aeruginosa carrying virulence genes. Methodology and results: Four clinical strains of P. aeruginosa CL1, CL2, CL3 and CL4 were obtained from Queen Elizabeth Hospital, Kota Kinabalu, Sabah. The bacterial strains were screened for virulence genes exoS, toxA and oprI and biofilm production. Six P. aeruginosa specific bacteriophages, namely PAtk1, PAtk2, PAtk3, PAtk4, PAtk5 and PAtk6, were isolated from Tasik Kejuruteraan, Universiti Kebangsaan Malaysia. These bacteriophages were screened for lytic spectrum against P. aeruginosa and two species of Enterobacteriaceae (Escherechia coli and Salmonella Typhi). PCR results showed that all strains possessed exoS, toxA and oprI genes except CL2 that lacked exoS. Nevertheless, it was CL2 that produced the highest biofilm density. Further, based on Transmission Electron Microscopy, PAtk15 and PAtk6 were classified into the family Myoviridae and Siphoviridae, respectively. Among all six isolated phages, only PAtk4 and PAtk6 showed the broadest lytic spectrum in which lytic activity was observed against all clinical P. aeruginosa strains. Conclusion, significance and impact of study: In this study we reported the isolation of six bacteriophages from Myoviridae and Siphoviridae that are specific to P. aeruginosa possessing exoS, toxA and oprI genes. Bacteriophages Patk4 and PAtk6 were able to infect all four strains of P. aeruginosa, making these phages potential agents in combating infections by the bacterium.

Original languageEnglish
Pages (from-to)563-569
Number of pages7
JournalMalaysian Journal of Microbiology
Volume14
Issue numberSpecialissue6
DOIs
Publication statusPublished - 1 Jan 2018

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Bacteriophages
Pseudomonas aeruginosa
Virulence
Myoviridae
Siphoviridae
Genes
Malaysia
Biofilms
Salmonella typhi
Enterobacteriaceae
Transmission Electron Microscopy
Bacteria
Polymerase Chain Reaction
Infection

Keywords

  • ExoS
  • Myoviridae
  • OprI
  • Siphoviridae
  • ToxA

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases

Cite this

Isolation and characterisation of phages targeting clinical Pseudomonas aeruginosa carrying virulence genes. / Binti Jamil, Nur Afifah; Baqer, Abeer Ameen; Adnan, Siti Noor Adnalizawati; Hussin, Ainulkhir; Md Nor, Norefrina Shafinaz.

In: Malaysian Journal of Microbiology, Vol. 14, No. Specialissue6, 01.01.2018, p. 563-569.

Research output: Contribution to journalArticle

Binti Jamil, Nur Afifah ; Baqer, Abeer Ameen ; Adnan, Siti Noor Adnalizawati ; Hussin, Ainulkhir ; Md Nor, Norefrina Shafinaz. / Isolation and characterisation of phages targeting clinical Pseudomonas aeruginosa carrying virulence genes. In: Malaysian Journal of Microbiology. 2018 ; Vol. 14, No. Specialissue6. pp. 563-569.
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