Abstract
Chitin is a microbe-associated molecular pattern (MAMP) molecule in fungal cell wall that functions as elicitor which induces immune response in plants. Plants have its own defence system to protect itself from any invasion of pathogens. Chitin Elicitor Binding Protein (CEBiP) is one of the plant pattern recognition receptors (PRRs) that will detect the presence of the pathogen-associated molecular patterns (PAMPs) and activate plant defence system. In this study, we used Oryza sativa variety UKMRC9 to study the function of Chitin Elicitor Binding Protein (CEBiP) gene in plant immune system. The sample was extracted and amplified using the primer that were specifically designed for CEBiP based on the full gene sequence obtained from MSU Rice Genome Annotation Project database. The sample then was transformed into pGEM-T cloning vector. The plasmid construct of vector pCAMBIA 1305.2 was completed using PmII and BstEII as the restriction enzyme. The sequencing results of pGEM-T-CEBiP-RC9 obtained are subjected to multiple sequence alignment and the generation of a phylogenetic tree. Analysis obtained from BLAST shows that CEBiP UKMRC9 has 100% identity with four sequences from NCBI database with high query coverage. Multiple sequence alignment also shows that there are some nucleotides that have been inserted and deleted (indels) in the amplified sequence. Pairwise alignment indicates high similarity between CEBiP UKMRC9 and Chitin elicitor binding protein [Oryza sativa Japonica Group], PREDICTED: chitin elicitor-binding protein [Oryza sativa Japonica Group], Chain A, Crystal Structure of Oscebip, Chain A, and Crystal Structure of Oscebip Complex in BLAST. Results from phylogeny analysis places CEBiP UKMRC9 under the same clade with Oryza sativa cv Nipponbare CEBiP MSU. Even within the graminaceae, there is cladal separation of organisms based on genus and species.
| Original language | English |
|---|---|
| Title of host publication | 2018 UKM FST Postgraduate Colloquium |
| Subtitle of host publication | Proceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2018 Postgraduate Colloquium |
| Editors | Noor Hayati Ahmad Rasol, Kamarulzaman Ibrahim, Siti Aishah Hasbullah, Mohammad Hafizuddin Hj. Jumali, Nazlina Ibrahim, Marlia Mohd Hanafiah, Mohd Talib Latif |
| Publisher | American Institute of Physics Inc. |
| ISBN (Electronic) | 9780735418431 |
| DOIs | |
| Publication status | Published - 27 Jun 2019 |
| Event | 2018 UKM FST Postgraduate Colloquium - Selangor, Malaysia Duration: 4 Apr 2018 → 6 Apr 2018 |
Publication series
| Name | AIP Conference Proceedings |
|---|---|
| Volume | 2111 |
| ISSN (Print) | 0094-243X |
| ISSN (Electronic) | 1551-7616 |
Conference
| Conference | 2018 UKM FST Postgraduate Colloquium |
|---|---|
| Country | Malaysia |
| City | Selangor |
| Period | 4/4/18 → 6/4/18 |
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Keywords
- Chitin Elicitor Binding Protein
- Oryza sativa
- Pathogen/Microbe-associated Molecular Pattern (PAMPs/MAMPs)
- pCAMBIA 1305.2
ASJC Scopus subject areas
- Ecology, Evolution, Behavior and Systematics
- Ecology
- Plant Science
- Physics and Astronomy(all)
- Nature and Landscape Conservation
Cite this
Isolation and characterisation of chitin elicitor binding protein (CEBiP) gene in Oryza sativa variety UKMRC9. / Malik, Nurul Azmina Binti Abdul; K. Nadarajah, Kalaivani.
2018 UKM FST Postgraduate Colloquium: Proceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2018 Postgraduate Colloquium. ed. / Noor Hayati Ahmad Rasol; Kamarulzaman Ibrahim; Siti Aishah Hasbullah; Mohammad Hafizuddin Hj. Jumali; Nazlina Ibrahim; Marlia Mohd Hanafiah; Mohd Talib Latif. American Institute of Physics Inc., 2019. 060019 (AIP Conference Proceedings; Vol. 2111).Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
}
TY - GEN
T1 - Isolation and characterisation of chitin elicitor binding protein (CEBiP) gene in Oryza sativa variety UKMRC9
AU - Malik, Nurul Azmina Binti Abdul
AU - K. Nadarajah, Kalaivani
PY - 2019/6/27
Y1 - 2019/6/27
N2 - Chitin is a microbe-associated molecular pattern (MAMP) molecule in fungal cell wall that functions as elicitor which induces immune response in plants. Plants have its own defence system to protect itself from any invasion of pathogens. Chitin Elicitor Binding Protein (CEBiP) is one of the plant pattern recognition receptors (PRRs) that will detect the presence of the pathogen-associated molecular patterns (PAMPs) and activate plant defence system. In this study, we used Oryza sativa variety UKMRC9 to study the function of Chitin Elicitor Binding Protein (CEBiP) gene in plant immune system. The sample was extracted and amplified using the primer that were specifically designed for CEBiP based on the full gene sequence obtained from MSU Rice Genome Annotation Project database. The sample then was transformed into pGEM-T cloning vector. The plasmid construct of vector pCAMBIA 1305.2 was completed using PmII and BstEII as the restriction enzyme. The sequencing results of pGEM-T-CEBiP-RC9 obtained are subjected to multiple sequence alignment and the generation of a phylogenetic tree. Analysis obtained from BLAST shows that CEBiP UKMRC9 has 100% identity with four sequences from NCBI database with high query coverage. Multiple sequence alignment also shows that there are some nucleotides that have been inserted and deleted (indels) in the amplified sequence. Pairwise alignment indicates high similarity between CEBiP UKMRC9 and Chitin elicitor binding protein [Oryza sativa Japonica Group], PREDICTED: chitin elicitor-binding protein [Oryza sativa Japonica Group], Chain A, Crystal Structure of Oscebip, Chain A, and Crystal Structure of Oscebip Complex in BLAST. Results from phylogeny analysis places CEBiP UKMRC9 under the same clade with Oryza sativa cv Nipponbare CEBiP MSU. Even within the graminaceae, there is cladal separation of organisms based on genus and species.
AB - Chitin is a microbe-associated molecular pattern (MAMP) molecule in fungal cell wall that functions as elicitor which induces immune response in plants. Plants have its own defence system to protect itself from any invasion of pathogens. Chitin Elicitor Binding Protein (CEBiP) is one of the plant pattern recognition receptors (PRRs) that will detect the presence of the pathogen-associated molecular patterns (PAMPs) and activate plant defence system. In this study, we used Oryza sativa variety UKMRC9 to study the function of Chitin Elicitor Binding Protein (CEBiP) gene in plant immune system. The sample was extracted and amplified using the primer that were specifically designed for CEBiP based on the full gene sequence obtained from MSU Rice Genome Annotation Project database. The sample then was transformed into pGEM-T cloning vector. The plasmid construct of vector pCAMBIA 1305.2 was completed using PmII and BstEII as the restriction enzyme. The sequencing results of pGEM-T-CEBiP-RC9 obtained are subjected to multiple sequence alignment and the generation of a phylogenetic tree. Analysis obtained from BLAST shows that CEBiP UKMRC9 has 100% identity with four sequences from NCBI database with high query coverage. Multiple sequence alignment also shows that there are some nucleotides that have been inserted and deleted (indels) in the amplified sequence. Pairwise alignment indicates high similarity between CEBiP UKMRC9 and Chitin elicitor binding protein [Oryza sativa Japonica Group], PREDICTED: chitin elicitor-binding protein [Oryza sativa Japonica Group], Chain A, Crystal Structure of Oscebip, Chain A, and Crystal Structure of Oscebip Complex in BLAST. Results from phylogeny analysis places CEBiP UKMRC9 under the same clade with Oryza sativa cv Nipponbare CEBiP MSU. Even within the graminaceae, there is cladal separation of organisms based on genus and species.
KW - Chitin Elicitor Binding Protein
KW - Oryza sativa
KW - Pathogen/Microbe-associated Molecular Pattern (PAMPs/MAMPs)
KW - pCAMBIA 1305.2
UR - http://www.scopus.com/inward/record.url?scp=85068431513&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85068431513&partnerID=8YFLogxK
U2 - 10.1063/1.5111281
DO - 10.1063/1.5111281
M3 - Conference contribution
T3 - AIP Conference Proceedings
BT - 2018 UKM FST Postgraduate Colloquium
A2 - Rasol, Noor Hayati Ahmad
A2 - Ibrahim, Kamarulzaman
A2 - Hasbullah, Siti Aishah
A2 - Jumali, Mohammad Hafizuddin Hj.
A2 - Ibrahim, Nazlina
A2 - Hanafiah, Marlia Mohd
A2 - Latif, Mohd Talib
PB - American Institute of Physics Inc.
ER -