Abstract
The isolation and characterization of a gene encoding the regulatory subunit of cAMP-dependent protein kinase (CgPKAR) of the fungal plant pathogen Colletotrichum gloeosporioides is described in this research. The CgPKAR gene and its corresponding cDNA have been cloned and sequenced. Sequence analysis revealed that CgPKAR contains one intron located at position -475 to -539 from the start codon (ATG). The gene encodes a protein comprising of 391 amino acids and showing 90% identity to Colletotrichum lagenarium protein kiase A regulatory subunit. The deduced CgPKAR contains predicted features of type II regulatory subunits which include a serine in the phosphorylation site, a kinase inhibitor domain and two cAMP-binding domains. Southern blot analyses indicated that CgPKAR is a single-copy gene in C. gloeosporioides. Real time RT-PCR analyses indicated that CgPKAR is higlily expressed in conidia and appressoria as compared to germinating conidia and mycelia.
| Original language | English |
|---|---|
| Pages (from-to) | 730-737 |
| Number of pages | 8 |
| Journal | Journal of Biological Sciences |
| Volume | 8 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 2008 |
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Keywords
- cAMP dependent protein kinase
- Colletotrichum gloeosporioides
- Gene expression
- Gene isolation
- Regulatory subunit
ASJC Scopus subject areas
- Cell Biology
- Molecular Medicine
Cite this
Isolation and characterisation of a gene encoding the Colletotrichum gloeosporioides regulatory subunit of protein kinase A. / Kamaruddin, Nurhaida; Abu Bakar, Farah Diba; Mahadi, Nor M.; Abd. Murad, Abdul Munir.
In: Journal of Biological Sciences, Vol. 8, No. 4, 2008, p. 730-737.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Isolation and characterisation of a gene encoding the Colletotrichum gloeosporioides regulatory subunit of protein kinase A
AU - Kamaruddin, Nurhaida
AU - Abu Bakar, Farah Diba
AU - Mahadi, Nor M.
AU - Abd. Murad, Abdul Munir
PY - 2008
Y1 - 2008
N2 - The isolation and characterization of a gene encoding the regulatory subunit of cAMP-dependent protein kinase (CgPKAR) of the fungal plant pathogen Colletotrichum gloeosporioides is described in this research. The CgPKAR gene and its corresponding cDNA have been cloned and sequenced. Sequence analysis revealed that CgPKAR contains one intron located at position -475 to -539 from the start codon (ATG). The gene encodes a protein comprising of 391 amino acids and showing 90% identity to Colletotrichum lagenarium protein kiase A regulatory subunit. The deduced CgPKAR contains predicted features of type II regulatory subunits which include a serine in the phosphorylation site, a kinase inhibitor domain and two cAMP-binding domains. Southern blot analyses indicated that CgPKAR is a single-copy gene in C. gloeosporioides. Real time RT-PCR analyses indicated that CgPKAR is higlily expressed in conidia and appressoria as compared to germinating conidia and mycelia.
AB - The isolation and characterization of a gene encoding the regulatory subunit of cAMP-dependent protein kinase (CgPKAR) of the fungal plant pathogen Colletotrichum gloeosporioides is described in this research. The CgPKAR gene and its corresponding cDNA have been cloned and sequenced. Sequence analysis revealed that CgPKAR contains one intron located at position -475 to -539 from the start codon (ATG). The gene encodes a protein comprising of 391 amino acids and showing 90% identity to Colletotrichum lagenarium protein kiase A regulatory subunit. The deduced CgPKAR contains predicted features of type II regulatory subunits which include a serine in the phosphorylation site, a kinase inhibitor domain and two cAMP-binding domains. Southern blot analyses indicated that CgPKAR is a single-copy gene in C. gloeosporioides. Real time RT-PCR analyses indicated that CgPKAR is higlily expressed in conidia and appressoria as compared to germinating conidia and mycelia.
KW - cAMP dependent protein kinase
KW - Colletotrichum gloeosporioides
KW - Gene expression
KW - Gene isolation
KW - Regulatory subunit
UR - http://www.scopus.com/inward/record.url?scp=60249090123&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=60249090123&partnerID=8YFLogxK
U2 - 10.3923/jbs.2008.730.737
DO - 10.3923/jbs.2008.730.737
M3 - Article
AN - SCOPUS:60249090123
VL - 8
SP - 730
EP - 737
JO - Journal of Biological Sciences
JF - Journal of Biological Sciences
SN - 1727-3048
IS - 4
ER -