Isolation and characterisation of a gene encoding the Colletotrichum gloeosporioides regulatory subunit of protein kinase A

Nurhaida Kamaruddin, Farah Diba Abu Bakar, Nor M. Mahadi, Abdul Munir Abd. Murad

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The isolation and characterization of a gene encoding the regulatory subunit of cAMP-dependent protein kinase (CgPKAR) of the fungal plant pathogen Colletotrichum gloeosporioides is described in this research. The CgPKAR gene and its corresponding cDNA have been cloned and sequenced. Sequence analysis revealed that CgPKAR contains one intron located at position -475 to -539 from the start codon (ATG). The gene encodes a protein comprising of 391 amino acids and showing 90% identity to Colletotrichum lagenarium protein kiase A regulatory subunit. The deduced CgPKAR contains predicted features of type II regulatory subunits which include a serine in the phosphorylation site, a kinase inhibitor domain and two cAMP-binding domains. Southern blot analyses indicated that CgPKAR is a single-copy gene in C. gloeosporioides. Real time RT-PCR analyses indicated that CgPKAR is higlily expressed in conidia and appressoria as compared to germinating conidia and mycelia.

Original languageEnglish
Pages (from-to)730-737
Number of pages8
JournalJournal of Biological Sciences
Volume8
Issue number4
DOIs
Publication statusPublished - 2008

Fingerprint

Colletotrichum
Cyclic AMP-Dependent Protein Kinases
Fungal Spores
Genes
Initiator Codon
Mycelium
Regulator Genes
Southern Blotting
Introns
Serine
Sequence Analysis
Real-Time Polymerase Chain Reaction
Proteins
Phosphotransferases
Complementary DNA
Phosphorylation
Amino Acids
Research

Keywords

  • cAMP dependent protein kinase
  • Colletotrichum gloeosporioides
  • Gene expression
  • Gene isolation
  • Regulatory subunit

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Medicine

Cite this

@article{8839f28247d04b87aa6eae8249fb81cb,
title = "Isolation and characterisation of a gene encoding the Colletotrichum gloeosporioides regulatory subunit of protein kinase A",
abstract = "The isolation and characterization of a gene encoding the regulatory subunit of cAMP-dependent protein kinase (CgPKAR) of the fungal plant pathogen Colletotrichum gloeosporioides is described in this research. The CgPKAR gene and its corresponding cDNA have been cloned and sequenced. Sequence analysis revealed that CgPKAR contains one intron located at position -475 to -539 from the start codon (ATG). The gene encodes a protein comprising of 391 amino acids and showing 90{\%} identity to Colletotrichum lagenarium protein kiase A regulatory subunit. The deduced CgPKAR contains predicted features of type II regulatory subunits which include a serine in the phosphorylation site, a kinase inhibitor domain and two cAMP-binding domains. Southern blot analyses indicated that CgPKAR is a single-copy gene in C. gloeosporioides. Real time RT-PCR analyses indicated that CgPKAR is higlily expressed in conidia and appressoria as compared to germinating conidia and mycelia.",
keywords = "cAMP dependent protein kinase, Colletotrichum gloeosporioides, Gene expression, Gene isolation, Regulatory subunit",
author = "Nurhaida Kamaruddin and {Abu Bakar}, {Farah Diba} and Mahadi, {Nor M.} and {Abd. Murad}, {Abdul Munir}",
year = "2008",
doi = "10.3923/jbs.2008.730.737",
language = "English",
volume = "8",
pages = "730--737",
journal = "Journal of Biological Sciences",
issn = "1727-3048",
publisher = "Asian Network for Scientific Information",
number = "4",

}

TY - JOUR

T1 - Isolation and characterisation of a gene encoding the Colletotrichum gloeosporioides regulatory subunit of protein kinase A

AU - Kamaruddin, Nurhaida

AU - Abu Bakar, Farah Diba

AU - Mahadi, Nor M.

AU - Abd. Murad, Abdul Munir

PY - 2008

Y1 - 2008

N2 - The isolation and characterization of a gene encoding the regulatory subunit of cAMP-dependent protein kinase (CgPKAR) of the fungal plant pathogen Colletotrichum gloeosporioides is described in this research. The CgPKAR gene and its corresponding cDNA have been cloned and sequenced. Sequence analysis revealed that CgPKAR contains one intron located at position -475 to -539 from the start codon (ATG). The gene encodes a protein comprising of 391 amino acids and showing 90% identity to Colletotrichum lagenarium protein kiase A regulatory subunit. The deduced CgPKAR contains predicted features of type II regulatory subunits which include a serine in the phosphorylation site, a kinase inhibitor domain and two cAMP-binding domains. Southern blot analyses indicated that CgPKAR is a single-copy gene in C. gloeosporioides. Real time RT-PCR analyses indicated that CgPKAR is higlily expressed in conidia and appressoria as compared to germinating conidia and mycelia.

AB - The isolation and characterization of a gene encoding the regulatory subunit of cAMP-dependent protein kinase (CgPKAR) of the fungal plant pathogen Colletotrichum gloeosporioides is described in this research. The CgPKAR gene and its corresponding cDNA have been cloned and sequenced. Sequence analysis revealed that CgPKAR contains one intron located at position -475 to -539 from the start codon (ATG). The gene encodes a protein comprising of 391 amino acids and showing 90% identity to Colletotrichum lagenarium protein kiase A regulatory subunit. The deduced CgPKAR contains predicted features of type II regulatory subunits which include a serine in the phosphorylation site, a kinase inhibitor domain and two cAMP-binding domains. Southern blot analyses indicated that CgPKAR is a single-copy gene in C. gloeosporioides. Real time RT-PCR analyses indicated that CgPKAR is higlily expressed in conidia and appressoria as compared to germinating conidia and mycelia.

KW - cAMP dependent protein kinase

KW - Colletotrichum gloeosporioides

KW - Gene expression

KW - Gene isolation

KW - Regulatory subunit

UR - http://www.scopus.com/inward/record.url?scp=60249090123&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=60249090123&partnerID=8YFLogxK

U2 - 10.3923/jbs.2008.730.737

DO - 10.3923/jbs.2008.730.737

M3 - Article

AN - SCOPUS:60249090123

VL - 8

SP - 730

EP - 737

JO - Journal of Biological Sciences

JF - Journal of Biological Sciences

SN - 1727-3048

IS - 4

ER -