Iso-Seq analysis of Nepenthes ampullaria, Nepenthes rafflesiana and Nepenthes × hookeriana for hybridisation study in pitcher plants

Muhammad Mu izzuddin Zulkapli, Muhammad Aqil Fitri Rosli, Faris Imadi Mohd Salleh, Normah Mohd. Noor, Wan Mohd Aizat Wan Kamaruddin, Hoe Han Goh

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Tropical pitcher plants in the species-rich Nepenthaceae family of carnivorous plants possess unique pitcher organs. Hybridisation, natural or artificial, in this family is extensive resulting in pitchers with diverse features. The pitcher functions as a passive insect trap with digestive fluid for nutrient acquisition in nitrogen-poor habitats. This organ shows specialisation according to the dietary habit of different Nepenthes species. In this study, we performed the first single-molecule real-time isoform sequencing (Iso-Seq) analysis of full-length cDNA from Nepenthes ampullaria which can feed on leaf litter, compared to carnivorous Nepenthes rafflesiana, and their carnivorous hybrid Nepenthes × hookeriana. This allows the comparison of pitcher transcriptomes from the parents and the hybrid to understand how hybridisation could shape the evolution of dietary habit in Nepenthes. Raw reads have been deposited to SRA database with the accession numbers SRX2692198 (N. ampullaria), SRX2692197 (N. rafflesiana), and SRX2692196 (N. × hookeriana).

Original languageEnglish
Pages (from-to)130-131
Number of pages2
JournalGenomics Data
Volume12
DOIs
Publication statusPublished - 1 Jun 2017

Fingerprint

Feeding Behavior
Nutrients
Protein Isoforms
Nitrogen
Complementary DNA
Molecules
Fluids
Transcriptome
Ecosystem
Insects
Databases

Keywords

  • Carnivorous plant
  • Iso-Seq
  • Nepenthes
  • PacBio SMRT
  • Pitcher
  • Transcriptome

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Medicine
  • Genetics

Cite this

Iso-Seq analysis of Nepenthes ampullaria, Nepenthes rafflesiana and Nepenthes × hookeriana for hybridisation study in pitcher plants. / Zulkapli, Muhammad Mu izzuddin; Rosli, Muhammad Aqil Fitri; Salleh, Faris Imadi Mohd; Mohd. Noor, Normah; Wan Kamaruddin, Wan Mohd Aizat; Goh, Hoe Han.

In: Genomics Data, Vol. 12, 01.06.2017, p. 130-131.

Research output: Contribution to journalArticle

Zulkapli, Muhammad Mu izzuddin ; Rosli, Muhammad Aqil Fitri ; Salleh, Faris Imadi Mohd ; Mohd. Noor, Normah ; Wan Kamaruddin, Wan Mohd Aizat ; Goh, Hoe Han. / Iso-Seq analysis of Nepenthes ampullaria, Nepenthes rafflesiana and Nepenthes × hookeriana for hybridisation study in pitcher plants. In: Genomics Data. 2017 ; Vol. 12. pp. 130-131.
@article{cfa09107e85d44cd9c1d70da7ee9f017,
title = "Iso-Seq analysis of Nepenthes ampullaria, Nepenthes rafflesiana and Nepenthes × hookeriana for hybridisation study in pitcher plants",
abstract = "Tropical pitcher plants in the species-rich Nepenthaceae family of carnivorous plants possess unique pitcher organs. Hybridisation, natural or artificial, in this family is extensive resulting in pitchers with diverse features. The pitcher functions as a passive insect trap with digestive fluid for nutrient acquisition in nitrogen-poor habitats. This organ shows specialisation according to the dietary habit of different Nepenthes species. In this study, we performed the first single-molecule real-time isoform sequencing (Iso-Seq) analysis of full-length cDNA from Nepenthes ampullaria which can feed on leaf litter, compared to carnivorous Nepenthes rafflesiana, and their carnivorous hybrid Nepenthes × hookeriana. This allows the comparison of pitcher transcriptomes from the parents and the hybrid to understand how hybridisation could shape the evolution of dietary habit in Nepenthes. Raw reads have been deposited to SRA database with the accession numbers SRX2692198 (N. ampullaria), SRX2692197 (N. rafflesiana), and SRX2692196 (N. × hookeriana).",
keywords = "Carnivorous plant, Iso-Seq, Nepenthes, PacBio SMRT, Pitcher, Transcriptome",
author = "Zulkapli, {Muhammad Mu izzuddin} and Rosli, {Muhammad Aqil Fitri} and Salleh, {Faris Imadi Mohd} and {Mohd. Noor}, Normah and {Wan Kamaruddin}, {Wan Mohd Aizat} and Goh, {Hoe Han}",
year = "2017",
month = "6",
day = "1",
doi = "10.1016/j.gdata.2017.05.003",
language = "English",
volume = "12",
pages = "130--131",
journal = "Genomics Data",
issn = "2213-5960",
publisher = "Elsevier Inc.",

}

TY - JOUR

T1 - Iso-Seq analysis of Nepenthes ampullaria, Nepenthes rafflesiana and Nepenthes × hookeriana for hybridisation study in pitcher plants

AU - Zulkapli, Muhammad Mu izzuddin

AU - Rosli, Muhammad Aqil Fitri

AU - Salleh, Faris Imadi Mohd

AU - Mohd. Noor, Normah

AU - Wan Kamaruddin, Wan Mohd Aizat

AU - Goh, Hoe Han

PY - 2017/6/1

Y1 - 2017/6/1

N2 - Tropical pitcher plants in the species-rich Nepenthaceae family of carnivorous plants possess unique pitcher organs. Hybridisation, natural or artificial, in this family is extensive resulting in pitchers with diverse features. The pitcher functions as a passive insect trap with digestive fluid for nutrient acquisition in nitrogen-poor habitats. This organ shows specialisation according to the dietary habit of different Nepenthes species. In this study, we performed the first single-molecule real-time isoform sequencing (Iso-Seq) analysis of full-length cDNA from Nepenthes ampullaria which can feed on leaf litter, compared to carnivorous Nepenthes rafflesiana, and their carnivorous hybrid Nepenthes × hookeriana. This allows the comparison of pitcher transcriptomes from the parents and the hybrid to understand how hybridisation could shape the evolution of dietary habit in Nepenthes. Raw reads have been deposited to SRA database with the accession numbers SRX2692198 (N. ampullaria), SRX2692197 (N. rafflesiana), and SRX2692196 (N. × hookeriana).

AB - Tropical pitcher plants in the species-rich Nepenthaceae family of carnivorous plants possess unique pitcher organs. Hybridisation, natural or artificial, in this family is extensive resulting in pitchers with diverse features. The pitcher functions as a passive insect trap with digestive fluid for nutrient acquisition in nitrogen-poor habitats. This organ shows specialisation according to the dietary habit of different Nepenthes species. In this study, we performed the first single-molecule real-time isoform sequencing (Iso-Seq) analysis of full-length cDNA from Nepenthes ampullaria which can feed on leaf litter, compared to carnivorous Nepenthes rafflesiana, and their carnivorous hybrid Nepenthes × hookeriana. This allows the comparison of pitcher transcriptomes from the parents and the hybrid to understand how hybridisation could shape the evolution of dietary habit in Nepenthes. Raw reads have been deposited to SRA database with the accession numbers SRX2692198 (N. ampullaria), SRX2692197 (N. rafflesiana), and SRX2692196 (N. × hookeriana).

KW - Carnivorous plant

KW - Iso-Seq

KW - Nepenthes

KW - PacBio SMRT

KW - Pitcher

KW - Transcriptome

UR - http://www.scopus.com/inward/record.url?scp=85019045895&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85019045895&partnerID=8YFLogxK

U2 - 10.1016/j.gdata.2017.05.003

DO - 10.1016/j.gdata.2017.05.003

M3 - Article

AN - SCOPUS:85019045895

VL - 12

SP - 130

EP - 131

JO - Genomics Data

JF - Genomics Data

SN - 2213-5960

ER -