Is plasma β-glucuronidase a novel human biomarker for monitoring anticholinesterase pesticides exposure? A Malaysian experience

Salmaan H. Inayat-Hussain, Syarif Husin Lubis, Noor Ibrahim Mohamed Sakian, Ahmad Rohi Ghazali, Noor Suhailah Ali, Magdi el Sersi, Lee Mun Toong, Awang Mat Zainal, Suhaimi Hashim, Mohd Shariman Ghazali, Mohd Nazri Saidin, Ab Razak Ab Rahman, Mohd Jamil Mohd Rafaai, Sollahudin Omar, Rafiah Rapiai, Radziah Othman, Lee Tiong Chan, Amran Johari, Wong Hing Soon, Abdul Rahim SallehTetsuo Satoh

Research output: Contribution to journalArticle

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Abstract

A cross-sectional study was conducted to investigate the effects of acute and chronic pesticide exposure on the plasma β-glucuronidase enzyme activity among five patients of acute pesticide poisoning in Tengku Ampuan Rahimah Hospital, Klang, 230 farmers in the MADA area, Kedah and 49 fishermen in Setiu, Terengganu. The duration of pesticide exposure among the patients was unknown, but the plasma samples from patients were collected on day one in the hospital. The duration of pesticide exposure among the farmers was between 1 and 45 years. The β-glucuronidase activity was compared with plasma cholinesterase activity in the same individual. The plasma cholinesterase activity was measured using Cholinesterase (PTC) Reagent set kit (Teco Diagnostics, UK) based on colorimetric method, while the plasma β-glucuronidase activity was measured fluorometrically based on β-glucuronidase assay. The plasma cholinesterase activity was significantly reduced (p < 0.05) among the patients (1386.786 ± 791.291 U/L/min) but the inhibition in plasma cholinesterase activity among the farmers (7346.5 ± 1860.786 U/L/min) was not significant (p > 0.05). The plasma β-glucuronidase activity among the farmers was significantly elevated (p < 0.05) (0.737 ± 0.425 μM/h) but not significant among the patients (p > 0.05). The plasma cholinesterase activity was positively correlated with the plasma β-glucuronidase activity among the farmers (r = 0.205, p < 0.01) but not among the patients (r = 0.79, p > 0.05). Thus, plasma β-glucuronidase enzyme activity can be measured as a biomarker for the chronic exposure of pesticide. However, further studies need to be performed to confirm whether plasma β-glucuronidase can be a sensitive biomarker for anticholinesterase pesticide poisoning.

Original languageEnglish
Pages (from-to)210-216
Number of pages7
JournalToxicology and Applied Pharmacology
Volume219
Issue number2-3
DOIs
Publication statusPublished - Mar 2007

Fingerprint

Glucuronidase
Cholinesterase Inhibitors
Biomarkers
Pesticides
Plasmas
Monitoring
Cholinesterases
Enzyme activity
Poisoning
Thermodynamic properties
Factor IX
Enzymes
Assays
Cross-Sectional Studies

Keywords

  • β-Glucuronidase
  • Anticholinesterase
  • Cholinesterase
  • Organophosphates

ASJC Scopus subject areas

  • Pharmacology
  • Toxicology

Cite this

Is plasma β-glucuronidase a novel human biomarker for monitoring anticholinesterase pesticides exposure? A Malaysian experience. / Inayat-Hussain, Salmaan H.; Lubis, Syarif Husin; Mohamed Sakian, Noor Ibrahim; Ghazali, Ahmad Rohi; Ali, Noor Suhailah; el Sersi, Magdi; Toong, Lee Mun; Zainal, Awang Mat; Hashim, Suhaimi; Ghazali, Mohd Shariman; Saidin, Mohd Nazri; Rahman, Ab Razak Ab; Rafaai, Mohd Jamil Mohd; Omar, Sollahudin; Rapiai, Rafiah; Othman, Radziah; Chan, Lee Tiong; Johari, Amran; Soon, Wong Hing; Salleh, Abdul Rahim; Satoh, Tetsuo.

In: Toxicology and Applied Pharmacology, Vol. 219, No. 2-3, 03.2007, p. 210-216.

Research output: Contribution to journalArticle

Inayat-Hussain, SH, Lubis, SH, Mohamed Sakian, NI, Ghazali, AR, Ali, NS, el Sersi, M, Toong, LM, Zainal, AM, Hashim, S, Ghazali, MS, Saidin, MN, Rahman, ARA, Rafaai, MJM, Omar, S, Rapiai, R, Othman, R, Chan, LT, Johari, A, Soon, WH, Salleh, AR & Satoh, T 2007, 'Is plasma β-glucuronidase a novel human biomarker for monitoring anticholinesterase pesticides exposure? A Malaysian experience', Toxicology and Applied Pharmacology, vol. 219, no. 2-3, pp. 210-216. https://doi.org/10.1016/j.taap.2006.10.014
Inayat-Hussain, Salmaan H. ; Lubis, Syarif Husin ; Mohamed Sakian, Noor Ibrahim ; Ghazali, Ahmad Rohi ; Ali, Noor Suhailah ; el Sersi, Magdi ; Toong, Lee Mun ; Zainal, Awang Mat ; Hashim, Suhaimi ; Ghazali, Mohd Shariman ; Saidin, Mohd Nazri ; Rahman, Ab Razak Ab ; Rafaai, Mohd Jamil Mohd ; Omar, Sollahudin ; Rapiai, Rafiah ; Othman, Radziah ; Chan, Lee Tiong ; Johari, Amran ; Soon, Wong Hing ; Salleh, Abdul Rahim ; Satoh, Tetsuo. / Is plasma β-glucuronidase a novel human biomarker for monitoring anticholinesterase pesticides exposure? A Malaysian experience. In: Toxicology and Applied Pharmacology. 2007 ; Vol. 219, No. 2-3. pp. 210-216.
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AU - Rahman, Ab Razak Ab

AU - Rafaai, Mohd Jamil Mohd

AU - Omar, Sollahudin

AU - Rapiai, Rafiah

AU - Othman, Radziah

AU - Chan, Lee Tiong

AU - Johari, Amran

AU - Soon, Wong Hing

AU - Salleh, Abdul Rahim

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