Ionizing radiation and DNA-chain elongation in ataxia telangiectasia lymphoblastoid cells

Rahmah Mohamed, Miriam Ford, Martin F. Lavin

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

DNA-chain elongation rates, determined by sedimentation analysis, were found to be similar in control and ataxia-telangiectasia lymphoblastoid cells. A γ-radiation dose of 6 Gray, which had previously been shown to have a marked inhibitory effect on initiation of DNA replication, had no appreciable effect on elongation rates in either cell type. Elongation rates were also determined at 20 Gray of γ-rays by pulsing cells with [3H]thymidine prior to irradiation to avoid anomalous sedimentation behaviour. At this radiation dose elongation was almost completely inhibited in control cells while little or no inhibition was observed in ataxia-telangiectasia cells. Deoxyribonucleoside triphosphate pool equilibration times were not altered at either dose.

Original languageEnglish
Pages (from-to)117-122
Number of pages6
JournalMutation Research DNA Repair Reports
Volume165
Issue number2
DOIs
Publication statusPublished - 1986
Externally publishedYes

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Ataxia Telangiectasia
Ionizing Radiation
DNA
Radiation
Deoxyribonucleosides
DNA Replication
Thymidine

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Ionizing radiation and DNA-chain elongation in ataxia telangiectasia lymphoblastoid cells. / Mohamed, Rahmah; Ford, Miriam; Lavin, Martin F.

In: Mutation Research DNA Repair Reports, Vol. 165, No. 2, 1986, p. 117-122.

Research output: Contribution to journalArticle

Mohamed, Rahmah ; Ford, Miriam ; Lavin, Martin F. / Ionizing radiation and DNA-chain elongation in ataxia telangiectasia lymphoblastoid cells. In: Mutation Research DNA Repair Reports. 1986 ; Vol. 165, No. 2. pp. 117-122.
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