Involvement of Seladin-1 in goniothalamin-induced apoptosis in urinary bladder cancer cells.

Heng K. Yen, Afifah Radiah Fauzi, Laily B. Din, Valerie J. McKelvey-Martin, Chan K. Meng, Salmaan H. Inayat-Hussain, Nor F. Rajab

Research output: Contribution to journalArticle

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Abstract

Selective Alzheimer Disease Indicator-1 (or Seladin-1) is a multifunctional protein first discovered by downregulation of its expression in Alzheimer's disease. Interestingly, the expression of this protein is upregulated in several cancers, including primary bladder cancer. However, its role in cancer formation has yet to be discovered. Goniothalamin is a natural product that has been demonstrated to induce apoptosis in various cancer cell lines. In this study, we have elucidated the role of Seladin-1 in goniothalamin-induced cytotoxicity towards human urinary bladder cancer cell line RT4. The cytotoxicity of goniothalamin in human urinary bladder cancer cell line RT4 was assessed using MTT assay and the mode of cell death was determined by Annexin V-FITC/PI labeling assay. Finally, the expression of Seladin-1 protein in goniothalamin-treated RT4 cells was determined by Western blot. MTT assay showed that the cytotoxicity of goniothalamin on RT4 cells was concentration and time dependent with IC50 values of 61 μM (24 hr), 38 μM (48 hr) and 31 μM for 72 hr, respectively. Cell death induced was confirmed through apoptosis; as assessed using the Annexin V-FITC/PI labeling assay. Furthermore, the involvement of Seladin-1 in goniothalamin-induced apoptosis was evidenced through the cleavage of 60 kDa protein to 40 kDa and 20 kDa. This was followed by a gradual increase of 20 kDa fragment suggesting the involvement of Seladin-1 in goniothalamin-induced apoptosis on RT4 cells. This study demonstrates that goniothalamin induce cytotoxicity and apoptosis on RT4 cells. The involvement of Seladin-1 in goniothalamin-induced apoptosis further suggested that Seladin-1 may play a role in the formation of primary bladder cancer.

Original languageEnglish
JournalBMC Complementary and Alternative Medicine
Volume14
DOIs
Publication statusPublished - 2014

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Urinary Bladder Neoplasms
Apoptosis
Fluorescein-5-isothiocyanate
Annexin A5
Cell Line
Proteins
Cell Death
goniothalamin
Neoplasms
Biological Products
Inhibitory Concentration 50
Alzheimer Disease
Down-Regulation
Western Blotting

ASJC Scopus subject areas

  • Complementary and alternative medicine

Cite this

Involvement of Seladin-1 in goniothalamin-induced apoptosis in urinary bladder cancer cells. / Yen, Heng K.; Fauzi, Afifah Radiah; Din, Laily B.; McKelvey-Martin, Valerie J.; Meng, Chan K.; Inayat-Hussain, Salmaan H.; Rajab, Nor F.

In: BMC Complementary and Alternative Medicine, Vol. 14, 2014.

Research output: Contribution to journalArticle

Yen, Heng K. ; Fauzi, Afifah Radiah ; Din, Laily B. ; McKelvey-Martin, Valerie J. ; Meng, Chan K. ; Inayat-Hussain, Salmaan H. ; Rajab, Nor F. / Involvement of Seladin-1 in goniothalamin-induced apoptosis in urinary bladder cancer cells. In: BMC Complementary and Alternative Medicine. 2014 ; Vol. 14.
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abstract = "Selective Alzheimer Disease Indicator-1 (or Seladin-1) is a multifunctional protein first discovered by downregulation of its expression in Alzheimer's disease. Interestingly, the expression of this protein is upregulated in several cancers, including primary bladder cancer. However, its role in cancer formation has yet to be discovered. Goniothalamin is a natural product that has been demonstrated to induce apoptosis in various cancer cell lines. In this study, we have elucidated the role of Seladin-1 in goniothalamin-induced cytotoxicity towards human urinary bladder cancer cell line RT4. The cytotoxicity of goniothalamin in human urinary bladder cancer cell line RT4 was assessed using MTT assay and the mode of cell death was determined by Annexin V-FITC/PI labeling assay. Finally, the expression of Seladin-1 protein in goniothalamin-treated RT4 cells was determined by Western blot. MTT assay showed that the cytotoxicity of goniothalamin on RT4 cells was concentration and time dependent with IC50 values of 61 μM (24 hr), 38 μM (48 hr) and 31 μM for 72 hr, respectively. Cell death induced was confirmed through apoptosis; as assessed using the Annexin V-FITC/PI labeling assay. Furthermore, the involvement of Seladin-1 in goniothalamin-induced apoptosis was evidenced through the cleavage of 60 kDa protein to 40 kDa and 20 kDa. This was followed by a gradual increase of 20 kDa fragment suggesting the involvement of Seladin-1 in goniothalamin-induced apoptosis on RT4 cells. This study demonstrates that goniothalamin induce cytotoxicity and apoptosis on RT4 cells. The involvement of Seladin-1 in goniothalamin-induced apoptosis further suggested that Seladin-1 may play a role in the formation of primary bladder cancer.",
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