Investigations on protease production by a wild-type Aspergillus terreus strain using diluted retentate of pre-filtered palm oil mill effluent (POME) as substrate

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Abstract

Production of protease by a local wild-type strain, Aspergillus terreus IMI 282743 using pre-filtered palm oil mill effluent (POME) and its derivatives was investigated. POME was first subjected to depth and surface filtration to remove the coarse solids found in the wastewater. Then, the pre-filtered POME was concentrated twofold to form retentate by means of ultrafiltration process. Maximum protease activity of 129 U/ml was achieved on the fourth day of fermentation in the medium containing 75% (v/v) retentate of pre-filtered POME as the sole carbon and nitrogen sources, operated at 37 °C, 250 rpm and 5% (v/v) of inoculum concentration. The results indicated that addition of nutrients and adjustment of initial pH were dispensable in this particular study to obtain better yield of protease. On the other hand, a decrease of about 59% and 19% of maximum protease activity found in the medium of pure retentate (100%, v/v) compared to the medium of 75% (v/v) and 50% (v/v) retentate, respectively, suggesting that retentate should be diluted prior to the fermentation process in order to gain better yield of protease.

Original languageEnglish
Pages (from-to)1223-1229
Number of pages7
JournalEnzyme and Microbial Technology
Volume39
Issue number6
DOIs
Publication statusPublished - 3 Oct 2006

Fingerprint

Palm oil
Aspergillus
Effluents
Peptide Hydrolases
Substrates
Fermentation
Ultrafiltration
Waste Water
Nutrients
Wastewater
Nitrogen
Carbon
palm oil
Derivatives
Food

Keywords

  • Aspergillus terreus
  • Palm oil mill effluent (POME)
  • Protease
  • Retentate
  • Ultrafiltration

ASJC Scopus subject areas

  • Biochemistry
  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

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title = "Investigations on protease production by a wild-type Aspergillus terreus strain using diluted retentate of pre-filtered palm oil mill effluent (POME) as substrate",
abstract = "Production of protease by a local wild-type strain, Aspergillus terreus IMI 282743 using pre-filtered palm oil mill effluent (POME) and its derivatives was investigated. POME was first subjected to depth and surface filtration to remove the coarse solids found in the wastewater. Then, the pre-filtered POME was concentrated twofold to form retentate by means of ultrafiltration process. Maximum protease activity of 129 U/ml was achieved on the fourth day of fermentation in the medium containing 75{\%} (v/v) retentate of pre-filtered POME as the sole carbon and nitrogen sources, operated at 37 °C, 250 rpm and 5{\%} (v/v) of inoculum concentration. The results indicated that addition of nutrients and adjustment of initial pH were dispensable in this particular study to obtain better yield of protease. On the other hand, a decrease of about 59{\%} and 19{\%} of maximum protease activity found in the medium of pure retentate (100{\%}, v/v) compared to the medium of 75{\%} (v/v) and 50{\%} (v/v) retentate, respectively, suggesting that retentate should be diluted prior to the fermentation process in order to gain better yield of protease.",
keywords = "Aspergillus terreus, Palm oil mill effluent (POME), Protease, Retentate, Ultrafiltration",
author = "Wu, {T. Y.} and Mohammad, {Abdul Wahab} and {Md Jahim}, Jamaliah and Nurina Anuar",
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