Investigation of the Effect of Supercritical Carbon Dioxide Pretreatment on Sugar Yield Prior to Enzymatic Hydrolysis of Empty Fruit Bunches

Nurul Hazirah Che Hamzah, Masturah Markom, Osman Hassan, Shuhaida Harun

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4 Citations (Scopus)

Abstract

Supercritical carbon dioxide (SC-CO2) pretreatment of empty fruit bunches (EFBs) was investigated for its effect on glucose yield following enzymatic hydrolysis. The SC-CO2 pretreatment of EFBs was performed under various conditions: temperature of 80°C or 130°C; pressure of 150, 200, or 250bar; moisture content of 0% or 75%; and time of 30min or 60min. The SC-CO2-pretreated EFBs were hydrolyzed using a combination of the enzymes Celluclast 1.5L and Novozyme 188 (Sigma Aldrich, St. Louis, MO) . The chemical compositions of untreated EFBs (% g/g dry biomass) were recorded as 36.7% cellulose, 22.8% hemicellulose, and 24.2% klason lignin. The optimal chemical composition of EFBs for SC-CO2 pretreatment were 38%, 25.5%, and 23.8% for cellulose, hemicellulose, and klason lignin, respectively. After enzymatic hydrolysis, the glucose yield of untreated EFBs was 17% (17g/100g dry biomass). SC-CO2 pretreatment of EFBs for 60min at 75% moisture content, 130°C, and 250 bar pressure gave the highest glucose yield of 24%. Pretreatment combined with premixing of the EFBs with alkali (SC-CO2 + alkaline) yielded glucose and xylose contents of 36.8% and 14.9%, respectively, The results showed an increase in glucose of about 13% for premixed pretreatment compared to SC-CO2 pretreatment alone. Morphology analysis showed changes on the surface of the treated EFBs, which looked swollen and ruptured compared with the surface of the untreated EFBs.

Original languageEnglish
Pages (from-to)272-276
Number of pages5
JournalIndustrial Biotechnology
Volume11
Issue number5
DOIs
Publication statusPublished - 1 Oct 2015

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Carbon Dioxide
Fruit
Hydrolysis
Glucose
Lignin
Cellulose
Biomass
Pressure
Alkalies
Temperature
Enzymes

Keywords

  • Empty fruit bunches
  • enzymatic hydrolysis
  • supercritical CO pretreatment

ASJC Scopus subject areas

  • Biotechnology

Cite this

@article{635d89babfe14751ba33f314da87c61c,
title = "Investigation of the Effect of Supercritical Carbon Dioxide Pretreatment on Sugar Yield Prior to Enzymatic Hydrolysis of Empty Fruit Bunches",
abstract = "Supercritical carbon dioxide (SC-CO2) pretreatment of empty fruit bunches (EFBs) was investigated for its effect on glucose yield following enzymatic hydrolysis. The SC-CO2 pretreatment of EFBs was performed under various conditions: temperature of 80°C or 130°C; pressure of 150, 200, or 250bar; moisture content of 0{\%} or 75{\%}; and time of 30min or 60min. The SC-CO2-pretreated EFBs were hydrolyzed using a combination of the enzymes Celluclast 1.5L and Novozyme 188 (Sigma Aldrich, St. Louis, MO) . The chemical compositions of untreated EFBs ({\%} g/g dry biomass) were recorded as 36.7{\%} cellulose, 22.8{\%} hemicellulose, and 24.2{\%} klason lignin. The optimal chemical composition of EFBs for SC-CO2 pretreatment were 38{\%}, 25.5{\%}, and 23.8{\%} for cellulose, hemicellulose, and klason lignin, respectively. After enzymatic hydrolysis, the glucose yield of untreated EFBs was 17{\%} (17g/100g dry biomass). SC-CO2 pretreatment of EFBs for 60min at 75{\%} moisture content, 130°C, and 250 bar pressure gave the highest glucose yield of 24{\%}. Pretreatment combined with premixing of the EFBs with alkali (SC-CO2 + alkaline) yielded glucose and xylose contents of 36.8{\%} and 14.9{\%}, respectively, The results showed an increase in glucose of about 13{\%} for premixed pretreatment compared to SC-CO2 pretreatment alone. Morphology analysis showed changes on the surface of the treated EFBs, which looked swollen and ruptured compared with the surface of the untreated EFBs.",
keywords = "Empty fruit bunches, enzymatic hydrolysis, supercritical CO pretreatment",
author = "{Che Hamzah}, {Nurul Hazirah} and Masturah Markom and Osman Hassan and Shuhaida Harun",
year = "2015",
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T1 - Investigation of the Effect of Supercritical Carbon Dioxide Pretreatment on Sugar Yield Prior to Enzymatic Hydrolysis of Empty Fruit Bunches

AU - Che Hamzah, Nurul Hazirah

AU - Markom, Masturah

AU - Hassan, Osman

AU - Harun, Shuhaida

PY - 2015/10/1

Y1 - 2015/10/1

N2 - Supercritical carbon dioxide (SC-CO2) pretreatment of empty fruit bunches (EFBs) was investigated for its effect on glucose yield following enzymatic hydrolysis. The SC-CO2 pretreatment of EFBs was performed under various conditions: temperature of 80°C or 130°C; pressure of 150, 200, or 250bar; moisture content of 0% or 75%; and time of 30min or 60min. The SC-CO2-pretreated EFBs were hydrolyzed using a combination of the enzymes Celluclast 1.5L and Novozyme 188 (Sigma Aldrich, St. Louis, MO) . The chemical compositions of untreated EFBs (% g/g dry biomass) were recorded as 36.7% cellulose, 22.8% hemicellulose, and 24.2% klason lignin. The optimal chemical composition of EFBs for SC-CO2 pretreatment were 38%, 25.5%, and 23.8% for cellulose, hemicellulose, and klason lignin, respectively. After enzymatic hydrolysis, the glucose yield of untreated EFBs was 17% (17g/100g dry biomass). SC-CO2 pretreatment of EFBs for 60min at 75% moisture content, 130°C, and 250 bar pressure gave the highest glucose yield of 24%. Pretreatment combined with premixing of the EFBs with alkali (SC-CO2 + alkaline) yielded glucose and xylose contents of 36.8% and 14.9%, respectively, The results showed an increase in glucose of about 13% for premixed pretreatment compared to SC-CO2 pretreatment alone. Morphology analysis showed changes on the surface of the treated EFBs, which looked swollen and ruptured compared with the surface of the untreated EFBs.

AB - Supercritical carbon dioxide (SC-CO2) pretreatment of empty fruit bunches (EFBs) was investigated for its effect on glucose yield following enzymatic hydrolysis. The SC-CO2 pretreatment of EFBs was performed under various conditions: temperature of 80°C or 130°C; pressure of 150, 200, or 250bar; moisture content of 0% or 75%; and time of 30min or 60min. The SC-CO2-pretreated EFBs were hydrolyzed using a combination of the enzymes Celluclast 1.5L and Novozyme 188 (Sigma Aldrich, St. Louis, MO) . The chemical compositions of untreated EFBs (% g/g dry biomass) were recorded as 36.7% cellulose, 22.8% hemicellulose, and 24.2% klason lignin. The optimal chemical composition of EFBs for SC-CO2 pretreatment were 38%, 25.5%, and 23.8% for cellulose, hemicellulose, and klason lignin, respectively. After enzymatic hydrolysis, the glucose yield of untreated EFBs was 17% (17g/100g dry biomass). SC-CO2 pretreatment of EFBs for 60min at 75% moisture content, 130°C, and 250 bar pressure gave the highest glucose yield of 24%. Pretreatment combined with premixing of the EFBs with alkali (SC-CO2 + alkaline) yielded glucose and xylose contents of 36.8% and 14.9%, respectively, The results showed an increase in glucose of about 13% for premixed pretreatment compared to SC-CO2 pretreatment alone. Morphology analysis showed changes on the surface of the treated EFBs, which looked swollen and ruptured compared with the surface of the untreated EFBs.

KW - Empty fruit bunches

KW - enzymatic hydrolysis

KW - supercritical CO pretreatment

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