Investigation of highly unsaturated fatty acid metabolism in the Asian sea bass, Lates calcarifer

Nurul Yuziana Mohd Yusof, O. Monroig, A. Mohd-Adnan, Kiew Lian Wan, D. R. Tocher

Research output: Contribution to journalArticle

63 Citations (Scopus)

Abstract

Lates calcarifer, commonly known as the Asian sea bass or barramundi, is an interesting species that has great aquaculture potential in Asia including Malaysia and also Australia. We have investigated essential fatty acid metabolism in this species, focusing on the endogenous highly unsaturated fatty acid (HUFA) synthesis pathway using both biochemical and molecular biological approaches. Fatty acyl desaturase (Fad) and elongase (Elovl) cDNAs were cloned and functional characterization identified them as {increment}6 Fad and Elovl5 elongase enzymes, respectively. The {increment}6 Fad was equally active toward 18:3n-3 and 18:2n-6, and Elovl5 exhibited elongation activity for C18-20 and C20-22 elongation and a trace of C22-24 activity. The tissue profile of gene expression for {increment}6 fad and elovl5 genes, showed brain to have the highest expression of both genes compared to all other tissues. The results of tissue fatty acid analysis showed that the brain contained more docosahexaenoic acid (DHA, 22:6n-3) than flesh, liver and intestine. The HUFA synthesis activity in isolated hepatocytes and enterocytes using [1-14C]18:3n-3 as substrate was very low with the only desaturated product detected being 18:4n-3. These findings indicate that L. calcarifer display an essential fatty acid pattern similar to other marine fish in that they appear unable to synthesize HUFA from C18 substrates. High expression of {increment}6 fad and elovl5 genes in brain may indicate a role for these enzymes in maintaining high DHA levels in neural tissues through conversion of 20:5n-3.

Original languageEnglish
Pages (from-to)827-843
Number of pages17
JournalFish Physiology and Biochemistry
Volume36
Issue number4
DOIs
Publication statusPublished - Dec 2010

Fingerprint

Bass
Lates calcarifer
highly unsaturated fatty acids
fatty acid metabolism
Unsaturated Fatty Acids
Metabolism
fatty acid
metabolism
Tissue
Brain
Essential Fatty Acids
Genes
essential fatty acids
brain
Elongation
Aquaculture
gene expression
synthesis
Docosahexaenoic Acids
Enterocytes

Keywords

  • cDNA
  • Desaturase
  • Elongase
  • Fatty acid
  • Functional characterization
  • Metabolism

ASJC Scopus subject areas

  • Aquatic Science
  • Biochemistry
  • Physiology

Cite this

Investigation of highly unsaturated fatty acid metabolism in the Asian sea bass, Lates calcarifer. / Mohd Yusof, Nurul Yuziana; Monroig, O.; Mohd-Adnan, A.; Wan, Kiew Lian; Tocher, D. R.

In: Fish Physiology and Biochemistry, Vol. 36, No. 4, 12.2010, p. 827-843.

Research output: Contribution to journalArticle

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