Investigating the protective actions of D-pinitol against arsenic-induced toxicity in PC12 cells and the underlying mechanism

Md Shiblur Rahaman, Mahmuda Akter, Md Mostafizur Rahman, Md Tajuddin Sikder, Toshiyuki Hosokawa, Takeshi Saito, Masaaki Kurasaki

Research output: Contribution to journalArticle

Abstract

Arsenic is awfully toxic metalloid responsible for many human diseases all over the world. Contrastingly, D-pinitol is a naturally occurring bioactive dietary compound has antioxidant properties. The objective of this study is to elucidate the protective actions of D-pinitol on arsenic-induced cytotoxicity and explore its controlling role in biomolecular mechanisms in PC12 cells. Obtained results demonstrated that co-exposure of D-pinitol with arsenic increases cell viability, decreases DNA damage and protects PC12 cells from arsenic-induced cytotoxicity by increasing glutathione (GSH) level and glutathione reductase (GR). Protein expression of western blot analysis showed that co-exposure of D-pinitol and arsenic significantly inhibited arsenic-induced autophagy which further suppressed apoptosis through up-regulation of survival factors; mTOR, p-mTOR, Akt, p-Akt, NF-кB, Nrf2, ERK1, GR, Bcl-x and down-regulation of death factors; p53, Bax, cytochrome c, LC3, although arsenic regulated those factors negatively. These results of this study suggested that D-pinitol protects PC12 cells from arsenic-induced cytotoxicity.

Original languageEnglish
Article number103302
JournalEnvironmental Toxicology and Pharmacology
Volume74
DOIs
Publication statusPublished - Feb 2020

Fingerprint

PC12 Cells
Arsenic
Toxicity
Cytotoxicity
Glutathione Reductase
Metalloids
pinitol
Poisons
Autophagy
Cytochromes c
DNA Damage
Glutathione
Cell Survival
Up-Regulation
Down-Regulation
Antioxidants
Western Blotting
Cells
Apoptosis
Survival

Keywords

  • Apoptosis
  • Arsenic
  • Autophagy
  • Cytotoxicity
  • D-pinitol
  • Glutathione reductase

ASJC Scopus subject areas

  • Toxicology
  • Pharmacology
  • Health, Toxicology and Mutagenesis

Cite this

Investigating the protective actions of D-pinitol against arsenic-induced toxicity in PC12 cells and the underlying mechanism. / Rahaman, Md Shiblur; Akter, Mahmuda; Rahman, Md Mostafizur; Sikder, Md Tajuddin; Hosokawa, Toshiyuki; Saito, Takeshi; Kurasaki, Masaaki.

In: Environmental Toxicology and Pharmacology, Vol. 74, 103302, 02.2020.

Research output: Contribution to journalArticle

Rahaman, Md Shiblur ; Akter, Mahmuda ; Rahman, Md Mostafizur ; Sikder, Md Tajuddin ; Hosokawa, Toshiyuki ; Saito, Takeshi ; Kurasaki, Masaaki. / Investigating the protective actions of D-pinitol against arsenic-induced toxicity in PC12 cells and the underlying mechanism. In: Environmental Toxicology and Pharmacology. 2020 ; Vol. 74.
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