International survey of Cronobacter sakazakii and other Cronobacter spp. in follow up formulas and infant foods

J. Chap, P. Jackson, R. Siqueira, N. Gaspar, C. Quintas, J. Park, T. Osaili, R. Shaker, Z. Jaradat, S. H P Hartantyo, Norrakiah Abdullah Sani, S. Estuningsih, S. J. Forsythe

Research output: Contribution to journalArticle

94 Citations (Scopus)

Abstract

A coordinated survey for Cronobacter and related organisms in powdered infant formula, follow up formula and infant foods was undertaken by 8 laboratories in 7 countries in recognition of and in response to the data needs identified in an FAO/WHO call for data in order to develop global risk management guidance for these products. The products (domestic and imported) were purchased from the local market and were categorised according to their principle ingredients. A total of 290 products were analysed using a standardised procedure of pre-enrichment in 225 ml Buffered Peptone Water (BPW), followed by enrichment in Enterobacteriaceae Enrichment (EE) broth, plating on the chromogenic Cronobacter Druggan-Forsythe-Iversen (DFI) agar and presumptive identification with ID 32 E. Presumptive Cronobacter isolates were identified using 16S rRNA gene sequence analysis. Aerobic plate counts (APC) of the products were also determined on nutrient agar. Fourteen samples had APC > 105 cfu/g, 3 of which contained probiotic cultures. C. sakazakii was isolated from 27 products; 3/91 (3%) follow up formulas (as defined by Codex Alimentarius Commission), and 24/199 (12%) infant foods and drinks. Hence C. sakazakii was less prevalent in follow up formula than other foods given to infants over the same age range. A range of other bacteria were also isolated from follow up formulas, including Acinetobacter baumannii, Enterobacter cloacae, Klebsiella pneumoniae, Citrobacter freundii, and Serratia ficaria. There was significant variation in the reconstitution instructions for follow up formulas. These included using water at temperatures which would enable bacterial growth. Additionally, the definition of follow up formula varied between countries.

Original languageEnglish
Pages (from-to)185-188
Number of pages4
JournalInternational Journal of Food Microbiology
Volume136
Issue number2
DOIs
Publication statusPublished - 31 Dec 2009

Fingerprint

Cronobacter
Cronobacter sakazakii
Infant Food
infant foods
Serratia ficaria
plate count
Agar
agar
Acinetobacter baumannii
Chromogenics
Citrobacter freundii
Serratia
Codex Alimentarius
Enterobacter cloacae
Food
Peptones
Infant Formula
Water
Klebsiella pneumoniae
risk management

Keywords

  • Cronobacter
  • Follow on formula
  • Follow up formula
  • Infant foods
  • Weaning foods

ASJC Scopus subject areas

  • Food Science
  • Microbiology
  • Safety, Risk, Reliability and Quality

Cite this

International survey of Cronobacter sakazakii and other Cronobacter spp. in follow up formulas and infant foods. / Chap, J.; Jackson, P.; Siqueira, R.; Gaspar, N.; Quintas, C.; Park, J.; Osaili, T.; Shaker, R.; Jaradat, Z.; Hartantyo, S. H P; Abdullah Sani, Norrakiah; Estuningsih, S.; Forsythe, S. J.

In: International Journal of Food Microbiology, Vol. 136, No. 2, 31.12.2009, p. 185-188.

Research output: Contribution to journalArticle

Chap, J, Jackson, P, Siqueira, R, Gaspar, N, Quintas, C, Park, J, Osaili, T, Shaker, R, Jaradat, Z, Hartantyo, SHP, Abdullah Sani, N, Estuningsih, S & Forsythe, SJ 2009, 'International survey of Cronobacter sakazakii and other Cronobacter spp. in follow up formulas and infant foods', International Journal of Food Microbiology, vol. 136, no. 2, pp. 185-188. https://doi.org/10.1016/j.ijfoodmicro.2009.08.005
Chap, J. ; Jackson, P. ; Siqueira, R. ; Gaspar, N. ; Quintas, C. ; Park, J. ; Osaili, T. ; Shaker, R. ; Jaradat, Z. ; Hartantyo, S. H P ; Abdullah Sani, Norrakiah ; Estuningsih, S. ; Forsythe, S. J. / International survey of Cronobacter sakazakii and other Cronobacter spp. in follow up formulas and infant foods. In: International Journal of Food Microbiology. 2009 ; Vol. 136, No. 2. pp. 185-188.
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