Interleukin-1 beta gene polymorphism in patients with systemic lupus erythematosus

M. R. Azizah, S. H. Kuak, N. C T Kong, Y. Normaznah

    Research output: Contribution to journalArticle

    1 Citation (Scopus)

    Abstract

    Background: Systemic lupus erythematosus (SLE) is a multisystemic autoimmune disease of unknown aetiology. Many studies have shown that multiple genes and environmental factors are responsible for the pathogenesis of the disease. Various immunological and clinical manifestations seen are characterized by inflammatory changes in different organ systems. Objective: The aims of this study were to investigate the frequency of the IL-1 beta (IL-1β) gene polymorphism in Chinese SLE patients and controls and to evaluate the relationship between IL-1β gene polymorphism and disease susceptibility and pathogenesis in patients. Materials and Methods: Ninety two SLE patients and 60 normal controls were genotyped at the promoter and exon-5 regions. Target DNA was amplified using the polymerase chain reaction and further digested using restriction enzymes, A va I and Taq I respectively. Results: We did not find any association in allele or genotype frequencies between SLE patients and controls. There was also no association of the IL1-beta promoter and exon-5 genotypes with clinical manifestations in SLE patients. Conclusion: We conclude that polymorphism of the IL1- beta gene at the promoter and exon-5 regions do not play a role in susceptibility and pathogenesis of Malaysian Chinese patients with SLE.

    Original languageEnglish
    Pages (from-to)5-8
    Number of pages4
    JournalInternational Medical Journal
    Volume13
    Issue number1
    Publication statusPublished - Mar 2006

    Fingerprint

    Interleukin-1beta
    Systemic Lupus Erythematosus
    Genes
    Exons
    Interleukin-1
    Genotype
    Disease Susceptibility
    Autoimmune Diseases
    Alleles
    Polymerase Chain Reaction
    DNA
    Enzymes

    Keywords

    • Gene polymorphism
    • IL-1 beta gene
    • Lupus

    ASJC Scopus subject areas

    • Medicine(all)

    Cite this

    Azizah, M. R., Kuak, S. H., Kong, N. C. T., & Normaznah, Y. (2006). Interleukin-1 beta gene polymorphism in patients with systemic lupus erythematosus. International Medical Journal, 13(1), 5-8.

    Interleukin-1 beta gene polymorphism in patients with systemic lupus erythematosus. / Azizah, M. R.; Kuak, S. H.; Kong, N. C T; Normaznah, Y.

    In: International Medical Journal, Vol. 13, No. 1, 03.2006, p. 5-8.

    Research output: Contribution to journalArticle

    Azizah, MR, Kuak, SH, Kong, NCT & Normaznah, Y 2006, 'Interleukin-1 beta gene polymorphism in patients with systemic lupus erythematosus', International Medical Journal, vol. 13, no. 1, pp. 5-8.
    Azizah, M. R. ; Kuak, S. H. ; Kong, N. C T ; Normaznah, Y. / Interleukin-1 beta gene polymorphism in patients with systemic lupus erythematosus. In: International Medical Journal. 2006 ; Vol. 13, No. 1. pp. 5-8.
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    AB - Background: Systemic lupus erythematosus (SLE) is a multisystemic autoimmune disease of unknown aetiology. Many studies have shown that multiple genes and environmental factors are responsible for the pathogenesis of the disease. Various immunological and clinical manifestations seen are characterized by inflammatory changes in different organ systems. Objective: The aims of this study were to investigate the frequency of the IL-1 beta (IL-1β) gene polymorphism in Chinese SLE patients and controls and to evaluate the relationship between IL-1β gene polymorphism and disease susceptibility and pathogenesis in patients. Materials and Methods: Ninety two SLE patients and 60 normal controls were genotyped at the promoter and exon-5 regions. Target DNA was amplified using the polymerase chain reaction and further digested using restriction enzymes, A va I and Taq I respectively. Results: We did not find any association in allele or genotype frequencies between SLE patients and controls. There was also no association of the IL1-beta promoter and exon-5 genotypes with clinical manifestations in SLE patients. Conclusion: We conclude that polymorphism of the IL1- beta gene at the promoter and exon-5 regions do not play a role in susceptibility and pathogenesis of Malaysian Chinese patients with SLE.

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